9129767 R4QPIR6K 1 apa 50 date desc year Allen, E. E. 18 https://eallen.scrippsprofiles.ucsd.edu/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A50%2C%22request_next%22%3A50%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%229RBYIELX%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Neu%20et%20al.%22%2C%22parsedDate%22%3A%222024-11-20%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENeu%2C%20A.%20T.%2C%20Torchin%2C%20M.%20E.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Roy%2C%20K.%20%282024%29.%20Microbiome%20divergence%20of%20marine%20gastropod%20species%20separated%20by%20the%20Isthmus%20of%20Panama.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E90%3C%5C%2Fi%3E%2811%29%2C%20e01003-24.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.01003-24%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.01003-24%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microbiome%20divergence%20of%20marine%20gastropod%20species%20separated%20by%20the%20Isthmus%20of%20Panama%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexander%20T.%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mark%20E.%22%2C%22lastName%22%3A%22Torchin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kaustuv%22%2C%22lastName%22%3A%22Roy%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Jennifer%20F.%22%2C%22lastName%22%3A%22Biddle%22%7D%5D%2C%22abstractNote%22%3A%22ABSTRACT%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20The%20rise%20of%20the%20Isthmus%20of%20Panama%20separated%20the%20populations%20of%20many%20marine%20organisms%2C%20which%20then%20diverged%20into%20new%20geminate%20sister%20species%20currently%20living%20in%20the%20Eastern%20Pacific%20Ocean%20and%20the%20Caribbean%20Sea.%20However%2C%20we%20know%20very%20little%20about%20how%20such%20evolutionary%20divergences%20of%20host%20species%20have%20shaped%20the%20compositions%20of%20their%20microbiomes.%20Here%2C%20we%20compared%20the%20microbiomes%20of%20whole-body%20and%20shell-surface%20samples%20of%20geminate%20species%20of%20marine%20gastropods%20in%20the%20genera%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Cerithium%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Cerithideopsis%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20to%20those%20of%20congeneric%20outgroups.%20Our%20results%20suggest%20that%20the%20effects%20of%20~3%20million%20years%20of%20separation%20and%20isolation%20on%20microbiome%20composition%20varied%20among%20host%20genera%20and%20between%20sample%20types%20within%20the%20same%20hosts.%20In%20the%20whole-body%20samples%2C%20microbiome%20compositions%20of%20geminate%20species%20pairs%20tended%20to%20be%20similar%2C%20likely%20due%20to%20host%20filtering%2C%20although%20the%20strength%20of%20this%20relationship%20varied%20among%20the%20two%20groups%20and%20across%20similarity%20metrics.%20Shell-surface%20microbiomes%20show%20contrasting%20patterns%2C%20with%20co-divergence%20between%20the%20host%20taxa%20and%20a%20small%20number%20of%20microbial%20clades%20evident%20in%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Cerithideopsis%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20but%20not%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Cerithium%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20These%20results%20suggest%20that%20%28i%29%20isolation%20of%20host%20populations%20after%20the%20rise%20of%20the%20Isthmus%20of%20Panama%20affected%20microbiomes%20of%20geminate%20hosts%20in%20a%20complex%20and%20host-specific%20manner%2C%20and%20%28ii%29%20host-associated%20microbial%20taxa%20respond%20differently%20to%20vicariance%20events%20than%20the%20hosts%20themselves.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20IMPORTANCE%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20While%20considerable%20work%20has%20been%20done%20on%20evolutionary%20divergences%20of%20marine%20species%20in%20response%20to%20the%20rise%20of%20the%20Isthmus%20of%20Panama%2C%20which%20separated%20two%20previously%20connected%20oceans%2C%20how%20this%20event%20shaped%20the%20microbiomes%20of%20these%20marine%20hosts%20remains%20poorly%20known.%20Using%20whole-body%20and%20shell-surface%20microbiomes%20of%20closely%20related%20gastropod%20species%20from%20opposite%20sides%20of%20the%20Isthmus%2C%20we%20show%20that%20divergences%20of%20microbial%20taxa%20after%20the%20formation%20of%20the%20Isthmus%20are%20often%20not%20concordant%20with%20those%20of%20their%20gastropod%20hosts.%20Our%20results%20show%20that%20evolutionary%20responses%20of%20marine%20gastropod-associated%20microbiomes%20to%20major%20environmental%20perturbations%20are%20complex%20and%20are%20shaped%20more%20by%20local%20environments%20than%20host%20evolutionary%20history.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20While%20considerable%20work%20has%20been%20done%20on%20evolutionary%20divergences%20of%20marine%20species%20in%20response%20to%20the%20rise%20of%20the%20Isthmus%20of%20Panama%2C%20which%20separated%20two%20previously%20connected%20oceans%2C%20how%20this%20event%20shaped%20the%20microbiomes%20of%20these%20marine%20hosts%20remains%20poorly%20known.%20Using%20whole-body%20and%20shell-surface%20microbiomes%20of%20closely%20related%20gastropod%20species%20from%20opposite%20sides%20of%20the%20Isthmus%2C%20we%20show%20that%20divergences%20of%20microbial%20taxa%20after%20the%20formation%20of%20the%20Isthmus%20are%20often%20not%20concordant%20with%20those%20of%20their%20gastropod%20hosts.%20Our%20results%20show%20that%20evolutionary%20responses%20of%20marine%20gastropod-associated%20microbiomes%20to%20major%20environmental%20perturbations%20are%20complex%20and%20are%20shaped%20more%20by%20local%20environments%20than%20host%20evolutionary%20history.%22%2C%22date%22%3A%222024-11-20%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1128%5C%2Faem.01003-24%22%2C%22ISSN%22%3A%220099-2240%2C%201098-5336%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2F10.1128%5C%2Faem.01003-24%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222024-12-20T17%3A52%3A53Z%22%7D%7D%2C%7B%22key%22%3A%22AL7B32WW%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Su%5Cu00e1rez-Moo%20et%20al.%22%2C%22parsedDate%22%3A%222024-07-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESu%26%23xE1%3Brez-Moo%2C%20P.%2C%20Garc%26%23xED%3Ba-Mart%26%23xED%3Bnez%2C%20N.%20C.%2C%20M%26%23xE1%3Brquez-Vel%26%23xE1%3Bzquez%2C%20N.%20A.%2C%20Figueroa%2C%20M.%2C%20Allen%2C%20E.%2C%20%26amp%3B%20Prieto-Dav%26%23xF3%3B%2C%20A.%20%282024%29.%20Exploring%20the%20microbial%20community%20and%20biotechnological%20potential%20of%20the%20sponge%20Xestospongia%20sp.%20from%20an%20anchialine%20cave%20in%20the%20Yucat%26%23xE1%3Bn%20Peninsula.%20%3Ci%3ECiencias%20Marinas%3C%5C%2Fi%3E%2C%20%3Ci%3E50%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7773%5C%2Fcm.y2024.3442%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7773%5C%2Fcm.y2024.3442%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Exploring%20the%20microbial%20community%20and%20biotechnological%20potential%20of%20the%20sponge%20Xestospongia%20sp.%20from%20an%20anchialine%20cave%20in%20the%20Yucat%5Cu00e1n%20Peninsula%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pablo%22%2C%22lastName%22%3A%22Su%5Cu00e1rez-Moo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ninette%20C%22%2C%22lastName%22%3A%22Garc%5Cu00eda-Mart%5Cu00ednez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Norma%20A%22%2C%22lastName%22%3A%22M%5Cu00e1rquez-Vel%5Cu00e1zquez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mario%22%2C%22lastName%22%3A%22Figueroa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alejandra%22%2C%22lastName%22%3A%22Prieto-Dav%5Cu00f3%22%7D%5D%2C%22abstractNote%22%3A%22Sponge-associated%20microorganisms%20are%20key%20influencers%20of%20nutrient%20biogeochemistry%20and%20important%20sources%20of%20bioactive%20natural%20products.%20This%20study%20provides%20the%20first%20insights%20into%20the%20taxonomic%20diversity%20of%20the%20microbial%20community%20associated%20with%20the%20sponge%20Xestospongia%20sp.%20from%20an%20anchialine%20cave%20in%20the%20coastal%20area%20of%20the%20underground%20river%20of%20the%20Yucat%5Cu00e1n%20Peninsula%20in%20Xcalak%2C%20Quintana%20Roo%2C%20Mexico%2C%20and%20the%20potential%20antimicrobial%20activity%20of%20its%20cultivable%20bacteria.%20High%20abundances%20of%20Sulfurospirillum%20and%20Desulfovibrio%20were%20detected%20with%2016S%20rRNA%20amplicons%2C%20suggesting%20that%20the%20microbial%20community%20of%20Xestospongia%20sp.%20plays%20an%20important%20role%20in%20the%20geochemical%20sulfur%20cycle.%20Analysis%20with%20crude%20extracts%20of%20Nocardiopsis%20dasonvillei%20NCA-454%20revealed%20antimicrobial%20activity%20against%20methicillin-sensitive%20Staphylococcus%20aureus%20ATCC%2025913%20%28MSSA%29%20and%20methicillin-%5Cu200bresistant%20%5Cu200bS.%20aureus%20ATCC%2043300%20%28MRSA%29.%20Studies%20of%20the%20microbiomes%20of%20sponges%20from%20the%20anchialine%20cave%20system%20in%20the%20Yucat%5Cu00e1n%20Peninsula%20can%20help%20elucidate%20the%20biogeochemical%20cycles%20of%20these%20poorly%20studied%20environments.%20Moreover%2C%20the%20microorganisms%20of%20these%20microbial%20communities%20represent%20an%20untapped%20source%20of%20secondary%20metabolites%20with%20biotechnological%20potential.%22%2C%22date%22%3A%222024-07-03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.7773%5C%2Fcm.y2024.3442%22%2C%22ISSN%22%3A%222395-9053%2C%200185-3880%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.cienciasmarinas.com.mx%5C%2Findex.php%5C%2Fcmarinas%5C%2Farticle%5C%2Fview%5C%2F3442%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222024-07-30T21%3A18%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22NE53RVQQ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Oliver%20et%20al.%22%2C%22parsedDate%22%3A%222024-03-27%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EOliver%2C%20A.%2C%20Podell%2C%20S.%2C%20Wegley%20Kelly%2C%20L.%2C%20Sparagon%2C%20W.%20J.%2C%20Plominsky%2C%20A.%20M.%2C%20Nelson%2C%20R.%20S.%2C%20Laurens%2C%20L.%20M.%20L.%2C%20Augyte%2C%20S.%2C%20Sims%2C%20N.%20A.%2C%20Nelson%2C%20C.%20E.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282024%29.%20Enrichable%20consortia%20of%20microbial%20symbionts%20degrade%20macroalgal%20polysaccharides%20in%20%3Ci%3EKyphosus%3C%5C%2Fi%3E%20fish.%20%3Ci%3EMBio%3C%5C%2Fi%3E%2C%20e00496-24.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmbio.00496-24%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmbio.00496-24%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Enrichable%20consortia%20of%20microbial%20symbionts%20degrade%20macroalgal%20polysaccharides%20in%20%3Ci%3EKyphosus%3C%5C%2Fi%3E%20fish%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aaron%22%2C%22lastName%22%3A%22Oliver%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%22%2C%22lastName%22%3A%22Wegley%20Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wesley%20J.%22%2C%22lastName%22%3A%22Sparagon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alvaro%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20S.%22%2C%22lastName%22%3A%22Nelson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lieve%20M.%20L.%22%2C%22lastName%22%3A%22Laurens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Augyte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neil%20A.%22%2C%22lastName%22%3A%22Sims%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Craig%20E.%22%2C%22lastName%22%3A%22Nelson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Jennifer%20B.%20H.%22%2C%22lastName%22%3A%22Martiny%22%7D%5D%2C%22abstractNote%22%3A%22ABSTRACT%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Coastal%20herbivorous%20fishes%20consume%20macroalgae%2C%20which%20is%20then%20degraded%20by%20microbes%20along%20their%20digestive%20tract.%20However%2C%20there%20is%20scarce%20genomic%20information%20about%20the%20microbiota%20that%20perform%20this%20degradation.%20This%20study%20explores%20the%20potential%20of%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20gastrointestinal%20microbial%20symbionts%20to%20collaboratively%20degrade%20and%20ferment%20polysaccharides%20from%20red%2C%20green%2C%20and%20brown%20macroalgae%20through%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20in%20silico%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20study%20of%20carbohydrate-active%20enzyme%20and%20sulfatase%20sequences.%20Recovery%20of%20metagenome-assembled%20genomes%20%28MAGs%29%20from%20previously%20described%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20gut%20metagenomes%20and%20newly%20sequenced%20bioreactor%20enrichments%20reveals%20differences%20in%20enzymatic%20capabilities%20between%20the%20major%20microbial%20taxa%20in%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20guts.%20The%20most%20versatile%20of%20the%20recovered%20MAGs%20were%20from%20the%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Bacteroidota%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20phylum%2C%20whose%20MAGs%20house%20enzyme%20collections%20able%20to%20decompose%20a%20variety%20of%20algal%20polysaccharides.%20Unique%20enzymes%20and%20predicted%20degradative%20capacities%20of%20genomes%20from%20the%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Bacillota%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28genus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Vallitalea%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%29%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Verrucomicrobiota%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28order%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kiritimatiellales%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%29%20highlight%20the%20importance%20of%20metabolic%20contributions%20from%20multiple%20phyla%20to%20broaden%20polysaccharide%20degradation%20capabilities.%20Few%20genomes%20contain%20the%20required%20enzymes%20to%20fully%20degrade%20any%20complex%20sulfated%20algal%20polysaccharide%20alone.%20The%20distribution%20of%20suitable%20enzymes%20between%20MAGs%20originating%20from%20different%20taxa%2C%20along%20with%20the%20widespread%20detection%20of%20signal%20peptides%20in%20candidate%20enzymes%2C%20is%20consistent%20with%20cooperative%20extracellular%20degradation%20of%20these%20carbohydrates.%20This%20study%20leverages%20genomic%20evidence%20to%20reveal%20an%20untapped%20diversity%20at%20the%20enzyme%20and%20strain%20level%20among%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20symbionts%20and%20their%20contributions%20to%20macroalgae%20decomposition.%20Bioreactor%20enrichments%20provide%20a%20genomic%20foundation%20for%20degradative%20and%20fermentative%20processes%20central%20to%20translating%20the%20knowledge%20gained%20from%20this%20system%20to%20the%20aquaculture%20and%20bioenergy%20sectors.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20IMPORTANCE%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Seaweed%20has%20long%20been%20considered%20a%20promising%20source%20of%20sustainable%20biomass%20for%20bioenergy%20and%20aquaculture%20feed%2C%20but%20scalable%20industrial%20methods%20for%20decomposing%20terrestrial%20compounds%20can%20struggle%20to%20break%20down%20seaweed%20polysaccharides%20efficiently%20due%20to%20their%20unique%20sulfated%20structures.%20Fish%20of%20the%20genus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20feed%20on%20seaweed%20by%20leveraging%20gastrointestinal%20bacteria%20to%20degrade%20algal%20polysaccharides%20into%20simple%20sugars.%20This%20study%20reconstructs%20metagenome-assembled%20genomes%20for%20these%20gastrointestinal%20bacteria%20to%20enhance%20our%20understanding%20of%20herbivorous%20fish%20digestion%20and%20fermentation%20of%20algal%20sugars.%20Investigations%20at%20the%20gene%20level%20identify%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20guts%20as%20an%20untapped%20source%20of%20seaweed-degrading%20enzymes%20ripe%20for%20further%20characterization.%20These%20discoveries%20set%20the%20stage%20for%20future%20work%20incorporating%20marine%20enzymes%20and%20microbial%20communities%20in%20the%20industrial%20degradation%20of%20algal%20polysaccharides.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Seaweed%20has%20long%20been%20considered%20a%20promising%20source%20of%20sustainable%20biomass%20for%20bioenergy%20and%20aquaculture%20feed%2C%20but%20scalable%20industrial%20methods%20for%20decomposing%20terrestrial%20compounds%20can%20struggle%20to%20break%20down%20seaweed%20polysaccharides%20efficiently%20due%20to%20their%20unique%20sulfated%20structures.%20Fish%20of%20the%20genus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20feed%20on%20seaweed%20by%20leveraging%20gastrointestinal%20bacteria%20to%20degrade%20algal%20polysaccharides%20into%20simple%20sugars.%20This%20study%20reconstructs%20metagenome-assembled%20genomes%20for%20these%20gastrointestinal%20bacteria%20to%20enhance%20our%20understanding%20of%20herbivorous%20fish%20digestion%20and%20fermentation%20of%20algal%20sugars.%20Investigations%20at%20the%20gene%20level%20identify%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20guts%20as%20an%20untapped%20source%20of%20seaweed-degrading%20enzymes%20ripe%20for%20further%20characterization.%20These%20discoveries%20set%20the%20stage%20for%20future%20work%20incorporating%20marine%20enzymes%20and%20microbial%20communities%20in%20the%20industrial%20degradation%20of%20algal%20polysaccharides.%22%2C%22date%22%3A%222024-03-27%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1128%5C%2Fmbio.00496-24%22%2C%22ISSN%22%3A%222150-7511%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2F10.1128%5C%2Fmbio.00496-24%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22DX7WZISK%22%5D%2C%22dateModified%22%3A%222024-04-11T23%3A25%3A03Z%22%7D%7D%2C%7B%22key%22%3A%2254TVY96U%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Podell%20et%20al.%22%2C%22parsedDate%22%3A%222023-05-31%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPodell%2C%20S.%2C%20Oliver%2C%20A.%2C%20Kelly%2C%20L.%20W.%2C%20Sparagon%2C%20W.%20J.%2C%20Plominsky%2C%20A.%20M.%2C%20Nelson%2C%20R.%20S.%2C%20Laurens%2C%20L.%20M.%20L.%2C%20Augyte%2C%20S.%2C%20Sims%2C%20N.%20A.%2C%20Nelson%2C%20C.%20E.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282023%29.%20Herbivorous%20Fish%20Microbiome%20Adaptations%20to%20Sulfated%20Dietary%20Polysaccharides.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E89%3C%5C%2Fi%3E%285%29%2C%20e02154-22.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.02154-22%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.02154-22%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Herbivorous%20Fish%20Microbiome%20Adaptations%20to%20Sulfated%20Dietary%20Polysaccharides%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aaron%22%2C%22lastName%22%3A%22Oliver%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%20Wegley%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wesley%20J.%22%2C%22lastName%22%3A%22Sparagon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alvaro%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20S.%22%2C%22lastName%22%3A%22Nelson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lieve%20M.%20L.%22%2C%22lastName%22%3A%22Laurens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Augyte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neil%20A.%22%2C%22lastName%22%3A%22Sims%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Craig%20E.%22%2C%22lastName%22%3A%22Nelson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Knut%22%2C%22lastName%22%3A%22Rudi%22%7D%5D%2C%22abstractNote%22%3A%22This%20work%20connects%20specific%20uncultured%20bacterial%20taxa%20with%20distinct%20polysaccharide%20digestion%20capabilities%20lacking%20in%20their%20marine%20vertebrate%20hosts%2C%20providing%20fresh%20insights%20into%20poorly%20understood%20processes%20for%20deconstructing%20complex%20sulfated%20polysaccharides%20and%20potential%20evolutionary%20mechanisms%20for%20microbial%20acquisition%20of%20expanded%20macroalgal%20utilization%20gene%20functions.%20Several%20thousand%20new%20marine-specific%20candidate%20enzyme%20sequences%20for%20polysaccharide%20utilization%20have%20been%20identified.%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20ABSTRACT%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Marine%20herbivorous%20fish%20that%20feed%20primarily%20on%20macroalgae%2C%20such%20as%20those%20from%20the%20genus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%2C%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20are%20essential%20for%20maintaining%20coral%20health%20and%20abundance%20on%20tropical%20reefs.%20Here%2C%20deep%20metagenomic%20sequencing%20and%20assembly%20of%20gut%20compartment-specific%20samples%20from%20three%20sympatric%2C%20macroalgivorous%20Hawaiian%20kyphosid%20species%20have%20been%20used%20to%20connect%20host%20gut%20microbial%20taxa%20with%20predicted%20protein%20functional%20capacities%20likely%20to%20contribute%20to%20efficient%20macroalgal%20digestion.%20Bacterial%20community%20compositions%2C%20algal%20dietary%20sources%2C%20and%20predicted%20enzyme%20functionalities%20were%20analyzed%20in%20parallel%20for%2016%20metagenomes%20spanning%20the%20mid-%20and%20hindgut%20digestive%20regions%20of%20wild-caught%20fishes.%20Gene%20colocalization%20patterns%20of%20expanded%20carbohydrate%20%28CAZy%29%20and%20sulfatase%20%28SulfAtlas%29%20digestive%20enzyme%20families%20on%20assembled%20contigs%20were%20used%20to%20identify%20likely%20polysaccharide%20utilization%20locus%20associations%20and%20to%20visualize%20potential%20cooperative%20networks%20of%20extracellularly%20exported%20proteins%20targeting%20complex%20sulfated%20polysaccharides.%20These%20insights%20into%20the%20gut%20microbiota%20of%20herbivorous%20marine%20fish%20and%20their%20functional%20capabilities%20improve%20our%20understanding%20of%20the%20enzymes%20and%20microorganisms%20involved%20in%20digesting%20complex%20macroalgal%20sulfated%20polysaccharides.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20IMPORTANCE%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20This%20work%20connects%20specific%20uncultured%20bacterial%20taxa%20with%20distinct%20polysaccharide%20digestion%20capabilities%20lacking%20in%20their%20marine%20vertebrate%20hosts%2C%20providing%20fresh%20insights%20into%20poorly%20understood%20processes%20for%20deconstructing%20complex%20sulfated%20polysaccharides%20and%20potential%20evolutionary%20mechanisms%20for%20microbial%20acquisition%20of%20expanded%20macroalgal%20utilization%20gene%20functions.%20Several%20thousand%20new%20marine-specific%20candidate%20enzyme%20sequences%20for%20polysaccharide%20utilization%20have%20been%20identified.%20These%20data%20provide%20foundational%20resources%20for%20future%20investigations%20into%20suppression%20of%20coral%20reef%20macroalgal%20overgrowth%2C%20fish%20host%20physiology%2C%20the%20use%20of%20macroalgal%20feedstocks%20in%20terrestrial%20and%20aquaculture%20animal%20feeds%2C%20and%20the%20bioconversion%20of%20macroalgae%20biomass%20into%20value-added%20commercial%20fuel%20and%20chemical%20products.%22%2C%22date%22%3A%222023-05-31%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1128%5C%2Faem.02154-22%22%2C%22ISSN%22%3A%220099-2240%2C%201098-5336%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2F10.1128%5C%2Faem.02154-22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222023-06-22T23%3A29%3A40Z%22%7D%7D%2C%7B%22key%22%3A%22R769TYT6%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Wilson%20et%20al.%22%2C%22parsedDate%22%3A%222023-02-28%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWilson%2C%20K.%2C%20De%20Rond%2C%20T.%2C%20Burkhardt%2C%20I.%2C%20Steele%2C%20T.%20S.%2C%20Sch%26%23xE4%3Bfer%2C%20R.%20J.%20B.%2C%20Podell%2C%20S.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Moore%2C%20B.%20S.%20%282023%29.%20Terpene%20biosynthesis%20in%20marine%20sponge%20animals.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%3C%5C%2Fi%3E%2C%20%3Ci%3E120%3C%5C%2Fi%3E%289%29%2C%20e2220934120.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2220934120%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2220934120%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Terpene%20biosynthesis%20in%20marine%20sponge%20animals%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kayla%22%2C%22lastName%22%3A%22Wilson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tristan%22%2C%22lastName%22%3A%22De%20Rond%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Immo%22%2C%22lastName%22%3A%22Burkhardt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Taylor%20S.%22%2C%22lastName%22%3A%22Steele%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%20J.%20B.%22%2C%22lastName%22%3A%22Sch%5Cu00e4fer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%5D%2C%22abstractNote%22%3A%22Sea%20sponges%20are%20the%20largest%20marine%20source%20of%20small-molecule%20natural%20products%20described%20to%20date.%20Sponge-derived%20molecules%2C%20such%20as%20the%20chemotherapeutic%20eribulin%2C%20the%20calcium-channel%20blocker%20manoalide%2C%20and%20antimalarial%20compound%20kalihinol%20A%2C%20are%20renowned%20for%20their%20impressive%20medicinal%2C%20chemical%2C%20and%20biological%20properties.%20Sponges%20contain%20microbiomes%20that%20control%20the%20production%20of%20many%20natural%20products%20isolated%20from%20these%20marine%20invertebrates.%20In%20fact%2C%20all%20genomic%20studies%20to%20date%20investigating%20the%20metabolic%20origins%20of%20sponge-derived%20small%20molecules%20concluded%20that%20microbes%5Cu2014not%20the%20sponge%20animal%20host%5Cu2014are%20the%20biosynthetic%20producers.%20However%2C%20early%20cell-sorting%20studies%20suggested%20the%20sponge%20animal%20host%20may%20play%20a%20role%20particularly%20in%20the%20production%20of%20terpenoid%20molecules.%20To%20investigate%20the%20genetic%20underpinnings%20of%20sponge%20terpenoid%20biosynthesis%2C%20we%20sequenced%20the%20metagenome%20and%20transcriptome%20of%20an%20isonitrile%20sesquiterpenoid-containing%20sponge%20of%20the%20order%20Bubarida.%20Using%20bioinformatic%20searches%20and%20biochemical%20validation%2C%20we%20identified%20a%20group%20of%20type%20I%20terpene%20synthases%20%28TSs%29%20from%20this%20sponge%20and%20multiple%20other%20species%2C%20the%20first%20of%20this%20enzyme%20class%20characterized%20from%20the%20sponge%20holobiome.%20The%20Bubarida%20TS-associated%20contigs%20consist%20of%20intron-containing%20genes%20homologous%20to%20sponge%20genes%20and%20feature%20GC%20percentage%20and%20coverage%20consistent%20with%20other%20eukaryotic%20sequences.%20We%20identified%20and%20characterized%20TS%20homologs%20from%20five%20different%20sponge%20species%20isolated%20from%20geographically%20distant%20locations%2C%20thereby%20suggesting%20a%20broad%20distribution%20amongst%20sponges.%20This%20work%20sheds%20light%20on%20the%20role%20of%20sponges%20in%20secondary%20metabolite%20production%20and%20speaks%20to%20the%20possibility%20that%20other%20sponge-specific%20molecules%20originate%20from%20the%20animal%20host.%22%2C%22date%22%3A%222023-02-28%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2220934120%22%2C%22ISSN%22%3A%220027-8424%2C%201091-6490%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fpnas.org%5C%2Fdoi%5C%2F10.1073%5C%2Fpnas.2220934120%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222024-03-06T00%3A57%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22SB94ACUR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222022-11-17%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20H%26%23xE4%3Brer%2C%20A.%2C%20Vechinski%2C%20J.%2C%20Frable%2C%20B.%20W.%2C%20Skelton%2C%20Z.%20R.%2C%20Kunselman%2C%20E.%2C%20Shane%2C%20M.%20A.%2C%20Perry%2C%20D.%20S.%2C%20Gonzalez%2C%20A.%2C%20McDonald%2C%20D.%2C%20Knight%2C%20R.%2C%20Michael%2C%20T.%20P.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282022%29.%20Host%20biology%2C%20ecology%20and%20the%20environment%20influence%20microbial%20biomass%20and%20diversity%20in%20101%20marine%20fish%20species.%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2C%20%3Ci%3E13%3C%5C%2Fi%3E%281%29%2C%206978.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-34557-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-34557-2%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Host%20biology%2C%20ecology%20and%20the%20environment%20influence%20microbial%20biomass%20and%20diversity%20in%20101%20marine%20fish%20species%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%22%2C%22lastName%22%3A%22H%5Cu00e4rer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joseph%22%2C%22lastName%22%3A%22Vechinski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benjamin%20W.%22%2C%22lastName%22%3A%22Frable%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zachary%20R.%22%2C%22lastName%22%3A%22Skelton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%22%2C%22lastName%22%3A%22Kunselman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20A.%22%2C%22lastName%22%3A%22Shane%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%20S.%22%2C%22lastName%22%3A%22Perry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonio%22%2C%22lastName%22%3A%22Gonzalez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22McDonald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Todd%20P.%22%2C%22lastName%22%3A%22Michael%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Fish%20are%20the%20most%20diverse%20and%20widely%20distributed%20vertebrates%2C%20yet%20little%20is%20known%20about%20the%20microbial%20ecology%20of%20fishes%20nor%20the%20biological%20and%20environmental%20factors%20that%20influence%20fish%20microbiota.%20To%20identify%20factors%20that%20explain%20microbial%20diversity%20patterns%20in%20a%20geographical%20subset%20of%20marine%20fish%2C%20we%20analyzed%20the%20microbiota%20%28gill%20tissue%2C%20skin%20mucus%2C%20midgut%20digesta%20and%20hindgut%20digesta%29%20from%20101%20species%20of%20Southern%20California%20marine%20fishes%2C%20spanning%2022%20orders%2C%2055%20families%20and%2083%20genera%2C%20representing%20~25%25%20of%20local%20marine%20fish%20diversity.%20We%20compare%20alpha%2C%20beta%20and%20gamma%20diversity%20while%20establishing%20a%20method%20to%20estimate%20microbial%20biomass%20associated%20with%20these%20host%20surfaces.%20We%20show%20that%20body%20site%20is%20the%20strongest%20driver%20of%20microbial%20diversity%20while%20microbial%20biomass%20and%20diversity%20is%20lowest%20in%20the%20gill%20of%20larger%2C%20pelagic%20fishes.%20Patterns%20of%20phylosymbiosis%20are%20observed%20across%20the%20gill%2C%20skin%20and%20hindgut.%20In%20a%20quantitative%20synthesis%20of%20vertebrate%20hindguts%20%28569%20species%29%2C%20we%20also%20show%20that%20mammals%20have%20the%20highest%20gamma%20diversity%20when%20controlling%20for%20host%20species%20number%20while%20fishes%20have%20the%20highest%20percent%20of%20unique%20microbial%20taxa.%20The%20composite%20dataset%20will%20be%20useful%20to%20vertebrate%20microbiota%20researchers%20and%20fish%20biologists%20interested%20in%20microbial%20ecology%2C%20with%20applications%20in%20aquaculture%20and%20fisheries%20management.%22%2C%22date%22%3A%222022-11-17%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-022-34557-2%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-022-34557-2%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22SNFNQMHP%22%5D%2C%22dateModified%22%3A%222022-12-02T17%3A24%3A23Z%22%7D%7D%2C%7B%22key%22%3A%22RES3PYDX%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Oliver%20et%20al.%22%2C%22parsedDate%22%3A%222022-07-14%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EOliver%2C%20A.%2C%20Cavalheri%2C%20H.%20B.%2C%20Lima%2C%20T.%20G.%2C%20Jones%2C%20N.%20T.%2C%20Podell%2C%20S.%2C%20Zarate%2C%20D.%2C%20Allen%2C%20E.%2C%20Burton%2C%20R.%20S.%2C%20%26amp%3B%20Shurin%2C%20J.%20B.%20%282022%29.%20Phenotypic%20and%20transcriptional%20response%20of%20Daphnia%20pulicaria%20to%20the%20combined%20effects%20of%20temperature%20and%20predation.%20%3Ci%3EPLOS%20ONE%3C%5C%2Fi%3E%2C%20%3Ci%3E17%3C%5C%2Fi%3E%287%29%2C%20e0265103.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0265103%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0265103%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Phenotypic%20and%20transcriptional%20response%20of%20Daphnia%20pulicaria%20to%20the%20combined%20effects%20of%20temperature%20and%20predation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aaron%22%2C%22lastName%22%3A%22Oliver%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hamanda%20B.%22%2C%22lastName%22%3A%22Cavalheri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thiago%20G.%22%2C%22lastName%22%3A%22Lima%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalie%20T.%22%2C%22lastName%22%3A%22Jones%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%22%2C%22lastName%22%3A%22Zarate%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ronald%20S.%22%2C%22lastName%22%3A%22Burton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%20B.%22%2C%22lastName%22%3A%22Shurin%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Syuhei%22%2C%22lastName%22%3A%22Ban%22%7D%5D%2C%22abstractNote%22%3A%22Daphnia%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20an%20ecologically%20important%20zooplankton%20species%20in%20lakes%2C%20shows%20both%20genetic%20adaptation%20and%20phenotypic%20plasticity%20in%20response%20to%20temperature%20and%20fish%20predation%2C%20but%20little%20is%20known%20about%20the%20molecular%20basis%20of%20these%20responses%20and%20their%20potential%20interactions.%20We%20performed%20a%20factorial%20experiment%20exposing%20laboratory-propagated%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Daphnia%20pulicaria%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20clones%20from%20two%20lakes%20in%20the%20Sierra%20Nevada%20mountains%20of%20California%20to%20normal%20or%20high%20temperature%20%2815%5Cu00b0C%20or%2025%5Cu00b0C%29%20in%20the%20presence%20or%20absence%20of%20fish%20kairomones%2C%20then%20measured%20changes%20in%20life%20history%20and%20gene%20expression.%20Exposure%20to%20kairomones%20increased%20upper%20thermal%20tolerance%20limits%20for%20physiological%20activity%20in%20both%20clones.%20Cloned%20individuals%20matured%20at%20a%20younger%20age%20in%20response%20to%20higher%20temperature%20and%20kairomones%2C%20while%20size%20at%20maturity%2C%20fecundity%20and%20population%20intrinsic%20growth%20were%20only%20affected%20by%20temperature.%20At%20the%20molecular%20level%2C%20both%20clones%20expressed%20more%20genes%20differently%20in%20response%20to%20temperature%20than%20predation%2C%20but%20specific%20genes%20involved%20in%20metabolic%2C%20cellular%2C%20and%20genetic%20processes%20responded%20differently%20between%20the%20two%20clones.%20Although%20gene%20expression%20differed%20more%20between%20clones%20from%20different%20lakes%20than%20experimental%20treatments%2C%20similar%20phenotypic%20responses%20to%20predation%20risk%20and%20warming%20arose%20from%20these%20clone-specific%20patterns.%20Our%20results%20suggest%20that%20phenotypic%20plasticity%20responses%20to%20temperature%20and%20kairomones%20interact%20synergistically%2C%20with%20exposure%20to%20fish%20predators%20increasing%20the%20tolerance%20of%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Daphnia%20pulicaria%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20to%20stressful%20temperatures%2C%20and%20that%20similar%20phenotypic%20responses%20to%20temperature%20and%20predator%20cues%20can%20be%20produced%20by%20divergent%20patterns%20of%20gene%20regulation.%22%2C%22date%22%3A%222022-7-14%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0265103%22%2C%22ISSN%22%3A%221932-6203%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdx.plos.org%5C%2F10.1371%5C%2Fjournal.pone.0265103%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22FNHP339V%22%5D%2C%22dateModified%22%3A%222023-05-03T16%3A02%3A51Z%22%7D%7D%2C%7B%22key%22%3A%22XZFW4HG9%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kunselman%20et%20al.%22%2C%22parsedDate%22%3A%222022-05-10%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKunselman%2C%20E.%2C%20Minich%2C%20J.%20J.%2C%20Horwith%2C%20M.%2C%20Gilbert%2C%20J.%20A.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282022%29.%20Variation%20in%20Survival%20and%20Gut%20Microbiome%20Composition%20of%20Hatchery-Grown%20Native%20Oysters%20at%20Various%20Locations%20within%20the%20Puget%20Sound.%20%3Ci%3EMicrobiology%20Spectrum%3C%5C%2Fi%3E%2C%20e01982-21.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fspectrum.01982-21%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fspectrum.01982-21%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Variation%20in%20Survival%20and%20Gut%20Microbiome%20Composition%20of%20Hatchery-Grown%20Native%20Oysters%20at%20Various%20Locations%20within%20the%20Puget%20Sound%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%22%2C%22lastName%22%3A%22Kunselman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Micah%22%2C%22lastName%22%3A%22Horwith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jack%20A.%22%2C%22lastName%22%3A%22Gilbert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Konstantinos%20Aristomenis%22%2C%22lastName%22%3A%22Kormas%22%7D%2C%7B%22creatorType%22%3A%22contributor%22%2C%22firstName%22%3A%22Timothy%22%2C%22lastName%22%3A%22Green%22%7D%5D%2C%22abstractNote%22%3A%22This%20is%20the%20first%20exploration%20of%20the%20microbial%20colonizers%20of%20the%20Olympia%20oyster%2C%20a%20native%20oyster%20species%20to%20the%20West%20Coast%2C%20which%20is%20a%20focus%20of%20restoration%20efforts.%20The%20patterns%20of%20differential%20microbial%20colonization%20by%20location%20reveal%20microscale%20characteristics%20of%20potential%20restoration%20sites%20which%20are%20not%20typically%20considered.%20These%20microbial%20dynamics%20can%20provide%20a%20more%20holistic%20perspective%20on%20the%20factors%20that%20may%20influence%20oyster%20performance.%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20ABSTRACT%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20The%20Olympia%20oyster%20%28%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Ostrea%20lurida%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%29%20of%20the%20Puget%20Sound%20suffered%20a%20dramatic%20population%20crash%2C%20but%20restoration%20efforts%20hope%20to%20revive%20this%20native%20species.%20One%20overlooked%20variable%20in%20the%20process%20of%20assessing%20ecosystem%20health%20is%20association%20of%20bacteria%20with%20marine%20organisms%20and%20the%20environments%20they%20occupy.%20Oyster%20microbiomes%20are%20known%20to%20differ%20significantly%20between%20species%2C%20tissue%20type%2C%20and%20the%20habitat%20in%20which%20they%20are%20found.%20The%20goals%20of%20this%20study%20were%20to%20determine%20the%20impact%20of%20field%20site%20and%20habitat%20on%20the%20oyster%20microbiome%20and%20to%20identify%20core%20oyster-associated%20bacteria%20in%20the%20Puget%20Sound.%20Olympia%20oysters%20from%20one%20parental%20family%20were%20deployed%20at%20four%20sites%20in%20the%20Puget%20Sound%20both%20inside%20and%20outside%20of%20eelgrass%20%28%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Zostera%20marina%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%29%20beds.%20Using%2016S%20rRNA%20gene%20amplicon%20sequencing%20of%20the%20oyster%20gut%2C%20shell%2C%20and%20surrounding%20seawater%20and%20sediment%2C%20we%20demonstrate%20that%20gut-associated%20bacteria%20are%20distinct%20from%20the%20surrounding%20environment%20and%20vary%20by%20field%20site.%20Furthermore%2C%20regional%20differences%20in%20the%20gut%20microbiota%20are%20associated%20with%20the%20survival%20rates%20of%20oysters%20at%20each%20site%20after%202%20months%20of%20field%20exposure.%20However%2C%20habitat%20type%20had%20no%20influence%20on%20microbiome%20diversity.%20Further%20work%20is%20needed%20to%20identify%20the%20specific%20bacterial%20dynamics%20that%20are%20associated%20with%20oyster%20physiology%20and%20survival%20rates.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20IMPORTANCE%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20This%20is%20the%20first%20exploration%20of%20the%20microbial%20colonizers%20of%20the%20Olympia%20oyster%2C%20a%20native%20oyster%20species%20to%20the%20West%20Coast%2C%20which%20is%20a%20focus%20of%20restoration%20efforts.%20The%20patterns%20of%20differential%20microbial%20colonization%20by%20location%20reveal%20microscale%20characteristics%20of%20potential%20restoration%20sites%20which%20are%20not%20typically%20considered.%20These%20microbial%20dynamics%20can%20provide%20a%20more%20holistic%20perspective%20on%20the%20factors%20that%20may%20influence%20oyster%20performance.%22%2C%22date%22%3A%222022-05-10%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1128%5C%2Fspectrum.01982-21%22%2C%22ISSN%22%3A%222165-0497%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2F10.1128%5C%2Fspectrum.01982-21%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2228H8GH9D%22%5D%2C%22dateModified%22%3A%222022-07-12T21%3A02%3A24Z%22%7D%7D%2C%7B%22key%22%3A%22JV9T3AG9%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Blanton%20et%20al.%22%2C%22parsedDate%22%3A%222022-04-27%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBlanton%2C%20J.%20M.%2C%20Peoples%2C%20L.%20M.%2C%20Gerringer%2C%20M.%20E.%2C%20Iacuaniello%2C%20C.%20M.%2C%20Gallo%2C%20N.%20D.%2C%20Linley%2C%20T.%20D.%2C%20Jamieson%2C%20A.%20J.%2C%20Drazen%2C%20J.%20C.%2C%20Bartlett%2C%20D.%20H.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282022%29.%20Microbiomes%20of%20Hadal%20Fishes%20across%20Trench%20Habitats%20Contain%20Similar%20Taxa%20and%20Known%20Piezophiles.%20%3Ci%3EMSphere%3C%5C%2Fi%3E%2C%20%3Ci%3E7%3C%5C%2Fi%3E%282%29%2C%20e00032-22.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmsphere.00032-22%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmsphere.00032-22%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microbiomes%20of%20Hadal%20Fishes%20across%20Trench%20Habitats%20Contain%20Similar%20Taxa%20and%20Known%20Piezophiles%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Logan%20M.%22%2C%22lastName%22%3A%22Peoples%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mackenzie%20E.%22%2C%22lastName%22%3A%22Gerringer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%20M.%22%2C%22lastName%22%3A%22Iacuaniello%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalya%20D.%22%2C%22lastName%22%3A%22Gallo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%20D.%22%2C%22lastName%22%3A%22Linley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alan%20J.%22%2C%22lastName%22%3A%22Jamieson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20C.%22%2C%22lastName%22%3A%22Drazen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Douglas%20H.%22%2C%22lastName%22%3A%22Bartlett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Barbara%20J.%22%2C%22lastName%22%3A%22Campbell%22%7D%5D%2C%22abstractNote%22%3A%22Hadal%20trenches%2C%20characterized%20by%20high%20hydrostatic%20pressures%20and%20low%20temperatures%2C%20are%20one%20of%20the%20most%20extreme%20environments%20on%20our%20planet.%20By%20examining%20the%20microbiome%20of%20abyssal%20and%20hadal%20fishes%2C%20we%20provide%20insight%20into%20the%20diversity%20and%20distribution%20of%20host-associated%20life%20at%20great%20depth.%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20ABSTRACT%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Hadal%20snailfishes%20are%20the%20deepest-living%20fishes%20in%20the%20ocean%2C%20inhabiting%20trenches%20from%20depths%20of%20%5Cu223c6%2C000%20to%208%2C000%20m.%20While%20the%20microbial%20communities%20in%20trench%20environments%20have%20begun%20to%20be%20characterized%2C%20the%20microbes%20associated%20with%20hadal%20megafauna%20remain%20relatively%20unknown.%20Here%2C%20we%20describe%20the%20gut%20microbiomes%20of%20two%20hadal%20snailfishes%2C%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Pseudoliparis%20swirei%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28Mariana%20Trench%29%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Notoliparis%20kermadecensis%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28Kermadec%20Trench%29%2C%20using%2016S%20rRNA%20gene%20amplicon%20sequencing.%20We%20contextualize%20these%20microbiomes%20with%20comparisons%20to%20the%20abyssal%20macrourid%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Coryphaenoides%20yaquinae%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20the%20continental%20shelf-dwelling%20snailfish%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Careproctus%20melanurus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20The%20microbial%20communities%20of%20the%20hadal%20snailfishes%20were%20distinct%20from%20their%20shallower%20counterparts%20and%20were%20dominated%20by%20the%20same%20sequences%20related%20to%20the%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Mycoplasmataceae%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Desulfovibrionaceae%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20These%20shared%20taxa%20indicate%20that%20symbiont%20lineages%20have%20remained%20similar%20to%20the%20ancestral%20symbiont%20since%20their%20geographic%20separation%20or%20that%20they%20are%20dispersed%20between%20geographically%20distant%20trenches%20and%20subsequently%20colonize%20specific%20hosts.%20The%20abyssal%20and%20hadal%20fishes%20contained%20sequences%20related%20to%20known%2C%20cultured%20piezophiles%2C%20microbes%20that%20grow%20optimally%20under%20high%20hydrostatic%20pressure%2C%20including%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Psychromonas%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Moritella%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Shewanella%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20These%20taxa%20are%20adept%20at%20colonizing%20nutrient-rich%20environments%20present%20in%20the%20deep%20ocean%2C%20such%20as%20on%20particles%20and%20in%20the%20guts%20of%20hosts%2C%20and%20we%20hypothesize%20they%20could%20make%20a%20dietary%20contribution%20to%20deep-sea%20fishes%20by%20degrading%20chitin%20and%20producing%20fatty%20acids.%20We%20characterize%20the%20gut%20microbiota%20within%20some%20of%20the%20deepest%20fishes%20to%20provide%20new%20insight%20into%20the%20diversity%20and%20distribution%20of%20host-associated%20microbial%20taxa%20and%20the%20potential%20of%20these%20animals%2C%20and%20the%20microbes%20they%20harbor%2C%20for%20understanding%20adaptation%20to%20deep-sea%20habitats.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20IMPORTANCE%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Hadal%20trenches%2C%20characterized%20by%20high%20hydrostatic%20pressures%20and%20low%20temperatures%2C%20are%20one%20of%20the%20most%20extreme%20environments%20on%20our%20planet.%20By%20examining%20the%20microbiome%20of%20abyssal%20and%20hadal%20fishes%2C%20we%20provide%20insight%20into%20the%20diversity%20and%20distribution%20of%20host-associated%20life%20at%20great%20depth.%20Our%20findings%20show%20that%20there%20are%20similar%20microbial%20populations%20in%20fishes%20geographically%20separated%20by%20thousands%20of%20miles%2C%20reflecting%20strong%20selection%20for%20specific%20microbial%20lineages.%20Only%20a%20few%20psychropiezophilic%20taxa%2C%20which%20do%20not%20reflect%20the%20diversity%20of%20microbial%20life%20at%20great%20depth%2C%20have%20been%20successfully%20isolated%20in%20the%20laboratory.%20Our%20examination%20of%20deep-sea%20fish%20microbiomes%20shows%20that%20typical%20high-pressure%20culturing%20methodologies%2C%20which%20have%20largely%20remained%20unchanged%20since%20the%20pioneering%20work%20of%20Claude%20ZoBell%20in%20the%201950s%2C%20may%20simulate%20the%20chemical%20environment%20found%20in%20animal%20guts%20and%20helps%20explain%20why%20the%20same%20deep-sea%20genera%20are%20consistently%20isolated.%22%2C%22date%22%3A%222022-04-27%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1128%5C%2Fmsphere.00032-22%22%2C%22ISSN%22%3A%222379-5042%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2F10.1128%5C%2Fmsphere.00032-22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22XBFFRTV5%22%5D%2C%22dateModified%22%3A%222022-07-14T15%3A41%3A02Z%22%7D%7D%2C%7B%22key%22%3A%227LLTJYR5%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sparagon%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESparagon%2C%20W.%20J.%2C%20Gentry%2C%20E.%20C.%2C%20Minich%2C%20J.%20J.%2C%20Vollbrecht%2C%20L.%2C%20Laurens%2C%20L.%20M.%20L.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Sims%2C%20N.%20A.%2C%20Dorrestein%2C%20P.%20C.%2C%20Kelly%2C%20L.%20W.%2C%20%26amp%3B%20Nelson%2C%20C.%20E.%20%282022%29.%20Fine%20scale%20transitions%20of%20the%20microbiota%20and%20metabolome%20along%20the%20gastrointestinal%20tract%20of%20herbivorous%20fishes.%20%3Ci%3EAnimal%20Microbiome%3C%5C%2Fi%3E%2C%20%3Ci%3E4%3C%5C%2Fi%3E%281%29%2C%2033.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs42523-022-00182-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs42523-022-00182-z%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Fine%20scale%20transitions%20of%20the%20microbiota%20and%20metabolome%20along%20the%20gastrointestinal%20tract%20of%20herbivorous%20fishes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wesley%20J.%22%2C%22lastName%22%3A%22Sparagon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%20C.%22%2C%22lastName%22%3A%22Gentry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lisa%22%2C%22lastName%22%3A%22Vollbrecht%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lieve%20M.%20L.%22%2C%22lastName%22%3A%22Laurens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neil%20A.%22%2C%22lastName%22%3A%22Sims%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pieter%20C.%22%2C%22lastName%22%3A%22Dorrestein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%20Wegley%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Craig%20E.%22%2C%22lastName%22%3A%22Nelson%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Background%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Gut%20microorganisms%20aid%20in%20the%20digestion%20of%20food%20by%20providing%20exogenous%20metabolic%20pathways%20to%20break%20down%20organic%20compounds.%20An%20integration%20of%20longitudinal%20microbial%20and%20chemical%20data%20is%20necessary%20to%20illuminate%20how%20gut%20microorganisms%20supplement%20the%20energetic%20and%20nutritional%20requirements%20of%20animals.%20Although%20mammalian%20gut%20systems%20are%20well-studied%20in%20this%20capacity%2C%20the%20role%20of%20microbes%20in%20the%20breakdown%20and%20utilization%20of%20recalcitrant%20marine%20macroalgae%20in%20herbivorous%20fish%20is%20relatively%20understudied%20and%20an%20emerging%20priority%20for%20bioproduct%20extraction.%20Here%20we%20use%20a%20comprehensive%20survey%20of%20the%20marine%20herbivorous%20fish%20gut%20microbial%20ecosystem%20via%20parallel%2016S%20rRNA%20gene%20amplicon%20profiling%20%28microbiota%29%20and%20untargeted%20tandem%20mass%20spectrometry%20%28metabolomes%29%20to%20demonstrate%20consistent%20transitions%20among%208%20gut%20subsections%20across%20five%20fish%20of%20the%20genus%20of%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Kyphosus%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Results%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Integration%20of%20microbial%20phylogenetic%20and%20chemical%20diversity%20data%20reveals%20that%20microbial%20communities%20and%20metabolomes%20covaried%20and%20differentiated%20continuously%20from%20stomach%20to%20hindgut%2C%20with%20the%20midgut%20containing%20multiple%20distinct%20and%20previously%20uncharacterized%20microenvironments%20and%20a%20distinct%20hindgut%20community%20dominated%20by%20obligate%20anaerobes.%20This%20differentiation%20was%20driven%20primarily%20by%20anaerobic%20gut%20endosymbionts%20of%20the%20classes%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Bacteroidia%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Clostridia%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20changing%20in%20concert%20with%20bile%20acids%2C%20small%20peptides%2C%20and%20phospholipids%3A%20bile%20acid%20deconjugation%20associated%20with%20early%20midgut%20microbiota%2C%20small%20peptide%20production%20associated%20with%20midgut%20microbiota%2C%20and%20phospholipid%20production%20associated%20with%20hindgut%20microbiota.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Conclusions%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20The%20combination%20of%20microbial%20and%20untargeted%20metabolomic%20data%20at%20high%20spatial%20resolution%20provides%20a%20new%20view%20of%20the%20diverse%20fish%20gut%20microenvironment%20and%20serves%20as%20a%20foundation%20to%20understand%20functional%20partitioning%20of%20microbial%20activities%20that%20contribute%20to%20the%20digestion%20of%20complex%20macroalgae%20in%20herbivorous%20marine%20fish.%22%2C%22date%22%3A%2212%5C%2F2022%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1186%5C%2Fs42523-022-00182-z%22%2C%22ISSN%22%3A%222524-4671%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fanimalmicrobiome.biomedcentral.com%5C%2Farticles%5C%2F10.1186%5C%2Fs42523-022-00182-z%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-06-17T23%3A41%3A22Z%22%7D%7D%2C%7B%22key%22%3A%22XFZ94J3E%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Klau%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKlau%2C%20L.%20J.%2C%20Podell%2C%20S.%2C%20Creamer%2C%20K.%20E.%2C%20Demko%2C%20A.%20M.%2C%20Singh%2C%20H.%20W.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Moore%2C%20B.%20S.%2C%20Ziemert%2C%20N.%2C%20Letzel%2C%20A.%20C.%2C%20%26amp%3B%20Jensen%2C%20P.%20R.%20%282022%29.%20The%20Natural%20Product%20Domain%20Seeker%20version%202%20%28NaPDoS2%29%20webtool%20relates%20ketosynthase%20phylogeny%20to%20biosynthetic%20function.%20%3Ci%3EJournal%20of%20Biological%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E298%3C%5C%2Fi%3E%2810%29%2C%20102480.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2022.102480%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2022.102480%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Natural%20Product%20Domain%20Seeker%20version%202%20%28NaPDoS2%29%20webtool%20relates%20ketosynthase%20phylogeny%20to%20biosynthetic%20function%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leesa%20J.%22%2C%22lastName%22%3A%22Klau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kaitlin%20E.%22%2C%22lastName%22%3A%22Creamer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alyssa%20M.%22%2C%22lastName%22%3A%22Demko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hans%20W.%22%2C%22lastName%22%3A%22Singh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nadine%22%2C%22lastName%22%3A%22Ziemert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%20Catrin%22%2C%22lastName%22%3A%22Letzel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%20R.%22%2C%22lastName%22%3A%22Jensen%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%2210%5C%2F2022%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jbc.2022.102480%22%2C%22ISSN%22%3A%2200219258%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flinkinghub.elsevier.com%5C%2Fretrieve%5C%2Fpii%5C%2FS0021925822009231%22%2C%22collections%22%3A%5B%22G2HWKXNT%22%2C%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222022-11-18T21%3A53%3A28Z%22%7D%7D%2C%7B%22key%22%3A%22LTP26QIR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Neu%20et%20al.%22%2C%22parsedDate%22%3A%222021-12%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENeu%2C%20A.%20T.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Roy%2C%20K.%20%282021%29.%20Defining%20and%20quantifying%20the%20core%20microbiome%3A%20Challenges%20and%20prospects.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E118%3C%5C%2Fi%3E%2851%29%2C%2010.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2104429118%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2104429118%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Defining%20and%20quantifying%20the%20core%20microbiome%3A%20Challenges%20and%20prospects%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20T.%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Roy%22%7D%5D%2C%22abstractNote%22%3A%22The%20term%20%5C%22core%20microbiome%5C%22%20has%20become%20widely%20used%20in%20microbial%20ecology%20over%20the%20last%20decade.%20Broadly%2C%20the%20core%20microbiome%20refers%20to%20any%20set%20of%20microbial%20taxa%2C%20or%20the%20genomic%20and%20functional%20attributes%20associated%20with%20those%20taxa%2C%20that%20are%20characteristic%20of%20a%20host%20or%20environment%20of%20interest.%20Most%20commonly%2C%20core%20microbiomes%20are%20measured%20as%20the%20microbial%20taxa%20shared%20among%20two%20or%20more%20samples%20from%20a%20particular%20host%20or%20environment.%20Despite%20the%20popularity%20of%20this%20term%20and%20its%20growing%20use%2C%20there%20is%20little%20consensus%20about%20how%20a%20core%20microbiome%20should%20be%20quantified%20in%20practice.%20Here%2C%20we%20present%20a%20brief%20history%20of%20the%20core%20microbiome%20concept%20and%20use%20a%20representative%20sample%20of%20the%20literature%20to%20review%20the%20different%20metrics%20commonly%20used%20for%20quantifying%20the%20core.%20Empirical%20analyses%20have%20used%20a%20wide%20range%20of%20metrics%20for%20quantifying%20the%20core%20microbiome%2C%20including%20arbitrary%20occurrence%20and%20abundance%20cutoff%20values%2C%20with%20the%20focal%20taxonomic%20level%20of%20the%20core%20ranging%20from%20phyla%20to%20amplicon%20sequence%20variants.%20However%2C%20many%20of%20these%20metrics%20are%20susceptible%20to%20sampling%20and%20other%20biases.%20Developing%20a%20standardized%20set%20of%20metrics%20for%20quantifying%20the%20core%20that%20accounts%20for%20such%20biases%20is%20necessary%20for%20testing%20specific%20hypotheses%20about%20the%20functional%20and%20ecological%20roles%20of%20core%20microbiomes.%22%2C%22date%22%3A%222021%5C%2F12%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2104429118%22%2C%22ISSN%22%3A%220027-8424%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22WU6NNTEW%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Neu%20et%20al.%22%2C%22parsedDate%22%3A%222021-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENeu%2C%20A.%20T.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Roy%2C%20K.%20%282021%29.%20Do%20host-associated%20microbes%20show%20a%20contrarian%20latitudinal%20diversity%20gradient%3F%20Insights%20from%20Mytilus%20californianus%2C%20an%20intertidal%20foundation%20host.%20%3Ci%3EJournal%20of%20Biogeography%3C%5C%2Fi%3E%2C%2014.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjbi.14243%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjbi.14243%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Do%20host-associated%20microbes%20show%20a%20contrarian%20latitudinal%20diversity%20gradient%3F%20Insights%20from%20Mytilus%20californianus%2C%20an%20intertidal%20foundation%20host%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20T.%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Roy%22%7D%5D%2C%22abstractNote%22%3A%22Aim%20The%20latitudinal%20diversity%20gradient%20%28LDG%29%20is%20one%20of%20the%20most%20pervasive%20large-scale%20trends%20in%20biodiversity.%20However%2C%20whether%20microbial%20taxa%20also%20conform%20to%20this%20pattern%20remains%20poorly%20known.%20This%20study%20uses%20the%20gill%20and%20shell-surface%20microbiomes%20of%20the%20marine%20bivalve%20Mytilus%20californianus%20to%20test%20whether%20the%20diversity%20of%20host-associated%20microbial%20communities%20declines%20with%20increasing%20latitude%2C%20as%20predicted%20by%20the%20LDG.%20Location%20Northeastern%20Pacific%20coast%2C%20ranging%20from%20Sitka%2C%20AK%20to%20La%20Jolla%2C%20CA%2C%20USA%20%28spanning%2024.12%20degrees%20of%20latitude%29.%20Taxon%20Bacteria%20and%20Archaea%20associated%20with%20M.%20californianus%20gills%20and%20shell-surfaces.%20Methods%20We%20amplified%20and%20sequenced%20the%2016S%20rRNA%20gene%20from%20M.%20californianus%20gill%20and%20shell-surface%20samples.%20We%20used%20linear%20and%20quadratic%20regressions%2C%20accounting%20for%20spatial%20autocorrelation%20when%20needed%2C%20of%20average%20alpha%20and%20gamma%20diversities%20of%20the%20whole%20microbiome%2C%20as%20well%20as%20individual%20microbial%20clades%2C%20to%20determine%20whether%20these%20taxa%20conformed%20to%20the%20traditional%20LDG.%20We%20also%20used%20permutational%20multivariate%20analysis%20of%20variance%20to%20determine%20the%20level%20of%20compositional%20differentiation%20among%20sampling%20sites.%20Results%20Gill%20and%20shell-surface%20microbiota%20show%20differing%20latitudinal%20diversity%20patterns%2C%20with%20both%20micro-environments%20exhibiting%20high%20levels%20of%20compositional%20differentiation%20along%20latitude.%20Individual%20microbial%20clades%20within%20each%20micro-environment%20also%20show%20different%20latitudinal%20trends%2C%20most%20likely%20due%20to%20differing%20ecologies%20and%20life%20histories.%20Main%20conclusions%20Mytilus%20californianus-associated%20microbial%20taxa%20show%20a%20flat%2C%20hump-shaped%20or%20contrarian%20LDG%2C%20suggesting%20that%20a%20steep%20decline%20in%20diversity%20with%20increasing%20latitude%20may%20not%20be%20a%20universal%20pattern%20across%20different%20domains%20of%20life.%22%2C%22date%22%3A%222021%5C%2F09%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1111%5C%2Fjbi.14243%22%2C%22ISSN%22%3A%220305-0270%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22SATZZFZU%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Oliver%20et%20al.%22%2C%22parsedDate%22%3A%222021-08%22%2C%22numChildren%22%3A6%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EOliver%2C%20A.%2C%20Podell%2C%20S.%2C%20Pinowska%2C%20A.%2C%20Traller%2C%20J.%20C.%2C%20Smith%2C%20S.%20R.%2C%20McClure%2C%20R.%2C%20Beliaev%2C%20A.%2C%20Bohutskyi%2C%20P.%2C%20Hill%2C%20E.%20A.%2C%20Rabines%2C%20A.%2C%20Zheng%2C%20H.%2C%20Allen%2C%20L.%20Z.%2C%20Kuo%2C%20A.%2C%20Grigoriev%2C%20I.%20V.%2C%20Allen%2C%20A.%20E.%2C%20Hazlebeck%2C%20D.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282021%29.%20Diploid%20genomic%20architecture%20of%20Nitzschia%20inconspicua%2C%20an%20elite%20biomass%20production%20diatom.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2014.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-95106-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-95106-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Diploid%20genomic%20architecture%20of%20Nitzschia%20inconspicua%2C%20an%20elite%20biomass%20production%20diatom%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Oliver%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Pinowska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20C.%22%2C%22lastName%22%3A%22Traller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20R.%22%2C%22lastName%22%3A%22Smith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22McClure%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Beliaev%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Bohutskyi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20A.%22%2C%22lastName%22%3A%22Hill%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Rabines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H.%22%2C%22lastName%22%3A%22Zheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20Z.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Kuo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20V.%22%2C%22lastName%22%3A%22Grigoriev%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Hazlebeck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22A%20near-complete%20diploid%20nuclear%20genome%20and%20accompanying%20circular%20mitochondrial%20and%20chloroplast%20genomes%20have%20been%20assembled%20from%20the%20elite%20commercial%20diatom%20species%20Nitzschia%20inconspicua.%20The%2050%20Mbp%20haploid%20size%20of%20the%20nuclear%20genome%20is%20nearly%20double%20that%20of%20model%20diatom%20Phaeodactylum%20tricornutum%2C%20but%2030%25%20smaller%20than%20closer%20relative%20Fragilariopsis%20cylindrus.%20Diploid%20assembly%2C%20which%20was%20facilitated%20by%20low%20levels%20of%20allelic%20heterozygosity%20%282.7%25%29%2C%20included%2014%20candidate%20chromosome%20pairs%20composed%20of%20long%2C%20syntenic%20contigs%2C%20covering%2093%25%20of%20the%20total%20assembly.%20Telomeric%20ends%20were%20capped%20with%20an%20unusual%2012-mer%2C%20G-rich%2C%20degenerate%20repeat%20sequence.%20Predicted%20proteins%20were%20highly%20enriched%20in%20strain-specific%20marker%20domains%20associated%20with%20cell-surface%20adhesion%2C%20biofilm%20formation%2C%20and%20raphe%20system%20gliding%20motility.%20Expanded%20species-specific%20families%20of%20carbonic%20anhydrases%20suggest%20potential%20enhancement%20of%20carbon%20concentration%20efficiency%2C%20and%20duplicated%20glycolysis%20and%20fatty%20acid%20synthesis%20pathways%20across%20cytosolic%20and%20organellar%20compartments%20may%20enhance%20peak%20metabolic%20output%2C%20contributing%20to%20competitive%20success%20over%20other%20organisms%20in%20mixed%20cultures.%20The%20N.%20inconspicua%20genome%20delivers%20a%20robust%20new%20reference%20for%20future%20functional%20and%20transcriptomic%20studies%20to%20illuminate%20the%20physiology%20of%20benthic%20pennate%20diatoms%20and%20harness%20their%20unique%20adaptations%20to%20support%20commercial%20algae%20biomass%20and%20bioproduct%20production.%22%2C%22date%22%3A%222021%5C%2F08%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-95106-3%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2286H9SNJB%22%2C%22WZ6VXZEN%22%5D%2C%22dateModified%22%3A%222022-09-19T23%3A02%3A00Z%22%7D%7D%2C%7B%22key%22%3A%22FSUEJHFV%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222021-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Poore%2C%20G.%20D.%2C%20Jantawongsri%2C%20K.%2C%20Johnston%2C%20C.%2C%20Bowie%2C%20K.%2C%20Bowman%2C%20J.%2C%20Knight%2C%20R.%2C%20Nowak%2C%20B.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Liu%2C%20S.-J.%20%282021%29.%20Microbial%20ecology%20of%20Atlantic%20salmon%20%28Salmo%20salar%29%20hatcheries%3A%20Impacts%20of%20the%20built%20environment%20on%20fish%20mucosal%20microbiota.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E86%3C%5C%2Fi%3E%2812%29%2C%20e00411-20.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FAEM.00411-20%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FAEM.00411-20%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microbial%20ecology%20of%20Atlantic%20salmon%20%28Salmo%20salar%29%20hatcheries%3A%20Impacts%20of%20the%20built%20environment%20on%20fish%20mucosal%20microbiota%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Greg%20D.%22%2C%22lastName%22%3A%22Poore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Khattapan%22%2C%22lastName%22%3A%22Jantawongsri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Colin%22%2C%22lastName%22%3A%22Johnston%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kate%22%2C%22lastName%22%3A%22Bowie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%22%2C%22lastName%22%3A%22Bowman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Nowak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shuang-Jiang%22%2C%22lastName%22%3A%22Liu%22%7D%5D%2C%22abstractNote%22%3A%22Successful%20rearing%20of%20fish%20in%20hatcheries%20is%20critical%20for%20conservation%2C%20recreational%20fishing%2C%20commercial%20fishing%20through%20wild%20stock%20enhancements%2C%20and%20aquaculture%20production.%20Flowthrough%20%28FT%29%20hatcheries%20require%20more%20water%20than%20recirculating%20aquaculture%20systems%20%28RAS%29%2C%20which%20enable%20up%20to%2099%25%20of%20their%20water%20to%20be%20recycled%2C%20thus%20significantly%20reducing%20environmental%20impacts.%20Here%2C%20we%20evaluated%20the%20biological%20and%20physical%20microbiome%20interactions%20of%20three%20Atlantic%20salmon%20hatcheries%20%28RAS%20n%5Cu2009%3D%5Cu20092%2C%20FT%20n%5Cu2009%3D%5Cu20091%29.%20Gill%2C%20skin%2C%20and%20digesta%20from%20six%20juvenile%20fish%20along%20with%20tank%20biofilms%20and%20water%20were%20sampled%20from%20tanks%20in%20each%20of%20the%20hatcheries%20%2860%20fish%20across%2010%20tanks%29%20to%20assess%20the%20built%20environment%20and%20mucosal%20microbiota%20using%2016S%20rRNA%20gene%20sequencing.%20The%20water%20and%20tank%20biofilm%20had%20more%20microbial%20richness%20than%20fish%20mucus%2C%20while%20skin%20and%20digesta%20from%20RAS%20fish%20had%202%20times%20the%20richness%20of%20FT%20fish.%20Body%20sites%20each%20had%20unique%20microbiomes%20%28P%5Cu2009%3C%5Cu20090.001%29%20and%20were%20influenced%20by%20hatchery%20system%20type%20%28P%5Cu2009%3C%5Cu20090.001%29%2C%20with%20RAS%20being%20more%20similar.%20A%20strong%20association%20between%20the%20tank%20and%20fish%20microbiome%20was%20observed.%20Water%20and%20tank%20biofilm%20richness%20was%20positively%20correlated%20with%20skin%20and%20digesta%20richness.%20Strikingly%2C%20the%20gill%2C%20skin%2C%20and%20digesta%20communities%20were%20more%20similar%20to%20that%20in%20the%20origin%20tank%20biofilm%20than%20those%20in%20all%20other%20experimental%20tanks%2C%20suggesting%20that%20the%20tank%20biofilm%20has%20a%20direct%20influence%20on%20fish-associated%20microbial%20communities.%20Lastly%2C%20microbial%20diversity%20and%20mucous%20cell%20density%20were%20positively%20associated%20with%20fish%20growth%20and%20length.%20The%20results%20from%20this%20study%20provide%20evidence%20for%20a%20link%20between%20the%20tank%20microbiome%20and%20the%20fish%20microbiome%2C%20with%20the%20skin%20microbiome%20as%20an%20important%20intermediate.%22%2C%22date%22%3A%222021%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FAEM.00411-20%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A20Z%22%7D%7D%2C%7B%22key%22%3A%223UMJ96RW%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Allemann%20et%20al.%22%2C%22parsedDate%22%3A%222021-05%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAllemann%2C%20M.%20N.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Atomi%2C%20H.%20%282021%29.%20Genetic%20suppression%20of%20lethal%20mutations%20in%20fatty%20acid%20biosynthesis%20mediated%20by%20a%20secondary%20lipid%20synthase.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E87%3C%5C%2Fi%3E%2812%29%2C%20e00035-21.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FAEM.00035-21%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FAEM.00035-21%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Genetic%20suppression%20of%20lethal%20mutations%20in%20fatty%20acid%20biosynthesis%20mediated%20by%20a%20secondary%20lipid%20synthase%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marco%20N.%22%2C%22lastName%22%3A%22Allemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Haruyuki%22%2C%22lastName%22%3A%22Atomi%22%7D%5D%2C%22abstractNote%22%3A%22The%20biosynthesis%20and%20incorporation%20of%20polyunsaturated%20fatty%20acids%20into%20phospholipid%20membranes%20are%20unique%20features%20of%20certain%20marine%20Gammaproteobacteria%20inhabiting%20high-pressure%20and%5C%2For%20low-temperature%20environments.%20In%20these%20bacteria%2C%20monounsaturated%20and%20saturated%20fatty%20acids%20are%20produced%20via%20the%20classical%20dissociated%20type%20II%20fatty%20acid%20synthase%20mechanism%2C%20while%20omega-3%20polyunsaturated%20fatty%20acids%20such%20as%20eicosapentaenoic%20acid%20%28EPA%3B%2020%3A5n-3%29%20and%20docosahexaenoic%20acid%20%28DHA%3B%2022%3A6n-3%29%20are%20produced%20by%20a%20hybrid%20polyketide%5C%2Ffatty%20acid%20synthase%5Cu2014encoded%20by%20the%20pfa%20genes%5Cu2014also%20referred%20to%20as%20the%20secondary%20lipid%20synthase%20mechanism.%20In%20this%20work%2C%20phenotypes%20associated%20with%20partial%20or%20complete%20loss%20of%20monounsaturated%20biosynthesis%20are%20shown%20to%20be%20compensated%20for%20by%20severalfold%20increased%20production%20of%20polyunsaturated%20fatty%20acids%20in%20the%20model%20marine%20bacterium%20Photobacterium%20profundum%20SS9.%20One%20route%20to%20suppression%20of%20these%20phenotypes%20could%20be%20achieved%20by%20transposition%20of%20insertion%20sequences%20within%20or%20upstream%20of%20the%20fabD%20coding%20sequence%2C%20which%20encodes%20malonyl%20coenzyme%20A%20%28malonyl-CoA%29%20acyl%20carrier%20protein%20transacylase.%20Genetic%20experiments%20in%20this%20strain%20indicated%20that%20fabD%20is%20not%20an%20essential%20gene%2C%20yet%20mutations%20in%20fabD%20and%20pfaA%20are%20synthetically%20lethal.%20Based%20on%20these%20results%2C%20we%20speculated%20that%20the%20malonyl-CoA%20transacylase%20domain%20within%20PfaA%20compensates%20for%20loss%20of%20FabD%20activity.%20Heterologous%20expression%20of%20either%20pfaABCD%20from%20P.%20profundum%20SS9%20or%20pfaABCDE%20from%20Shewanella%20pealeana%20in%20Escherichia%20coli%20complemented%20the%20loss%20of%20the%20chromosomal%20copy%20of%20fabD%20in%20vivo.%20The%20co-occurrence%20of%20independent%2C%20yet%20compensatory%2C%20fatty%20acid%20biosynthetic%20pathways%20in%20selected%20marine%20bacteria%20may%20provide%20genetic%20redundancy%20to%20optimize%20fitness%20under%20extreme%20conditions.%22%2C%22date%22%3A%222021%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FAEM.00035-21%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-08-05T16%3A10%3A14Z%22%7D%7D%2C%7B%22key%22%3A%227HTJU83G%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Petras%20et%20al.%22%2C%22parsedDate%22%3A%222021-05%22%2C%22numChildren%22%3A6%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPetras%2C%20D.%2C%20Minich%2C%20J.%20J.%2C%20Cancelada%2C%20L.%20B.%2C%20Torres%2C%20R.%20R.%2C%20Kunselman%2C%20E.%2C%20Wang%2C%20M.%20X.%2C%20White%2C%20M.%20E.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Prather%2C%20K.%20A.%2C%20Aluwihare%2C%20L.%20I.%2C%20%26amp%3B%20Dorrestein%2C%20P.%20C.%20%282021%29.%20Non-targeted%20tandem%20mass%20spectrometry%20enables%20the%20visualization%20of%20organic%20matter%20chemotype%20shifts%20in%20coastal%20seawater.%20%3Ci%3EChemosphere%3C%5C%2Fi%3E%2C%20%3Ci%3E271%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.chemosphere.2020.129450%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.chemosphere.2020.129450%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Non-targeted%20tandem%20mass%20spectrometry%20enables%20the%20visualization%20of%20organic%20matter%20chemotype%20shifts%20in%20coastal%20seawater%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Petras%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20B.%22%2C%22lastName%22%3A%22Cancelada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20R.%22%2C%22lastName%22%3A%22Torres%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Kunselman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20X.%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20E.%22%2C%22lastName%22%3A%22White%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Prather%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20I.%22%2C%22lastName%22%3A%22Aluwihare%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20C.%22%2C%22lastName%22%3A%22Dorrestein%22%7D%5D%2C%22abstractNote%22%3A%22Urbanization%20along%20coastlines%20alters%20marine%20ecosystems%20including%20contributing%20molecules%20of%20anthropogenic%20origin%20to%20the%20coastal%20dissolved%20organic%20matter%20%28DOM%29%20pool.%20A%20broad%20assessment%20of%20the%20nature%20and%20extent%20of%20anthropogenic%20impacts%20on%20coastal%20ecosystems%20is%20urgently%20needed%20to%20inform%20regulatory%20guidelines%20and%20ecosystem%20management.%20Recently%2C%20non-targeted%20tandem%20mass%20spectrometry%20approaches%20are%20gaining%20momentum%20for%20the%20analysis%20of%20global%20organic%20matter%20composition%20%28chemotypes%29%20including%20a%20wide%20array%20of%20natural%20and%20anthropogenic%20compounds.%20In%20line%20with%20these%20efforts%2C%20we%20developed%20a%20non-targeted%20liquid%20chromatography%20tandem%20mass%20spectrometry%20%28LC-MS%5C%2FMS%29%20workflow%20that%20utilizes%20advanced%20data%20analysis%20approaches%20such%20as%20feature-based%20molecular%20networking%20and%20repository-scale%20spectrum%20searches.%20This%20workflow%20allows%20the%20scalable%20comparison%20and%20mapping%20of%20seawater%20chemotypes%20from%20large-scale%20spatial%20surveys%20as%20well%20as%20molecular%20family%20level%20annotation%20of%20unknown%20compounds.%20As%20a%20case%20study%2C%20we%20visualized%20organic%20matter%20chemotype%20shifts%20in%20coastal%20environments%20in%20northern%20San%20Diego%2C%20USA%2C%20after%20notable%20rain%20fall%20in%20winter%202017%5C%2F2018%20and%20highlight%20potential%20anthropogenic%20impacts.%20The%20observed%20seawater%20chemotype%2C%20consisting%20of%204384%20LC-MS%5C%2FMS%20features%2C%20shifted%20significantly%20after%20a%20major%20rain%20event.%20Molecular%20drivers%20of%20this%20shift%20could%20be%20attributed%20to%20multiple%20anthropogenic%20compounds%2C%20including%20pesticides%20%28Imazapyr%20and%20Isoxaben%29%2C%20cleaning%20products%20%28Benzyl-tetradecyl-dimethylammonium%29%20and%20chemical%20additives%20%28Hexa%20%28methoxymethyl%29melamine%29%20and%20potential%20degradation%20products.%20By%20expanding%20the%20search%20of%20identified%20xenobiotics%20to%20other%20public%20tandem%20mass%20spectrometry%20datasets%2C%20we%20further%20contextualized%20their%20possible%20origin%20and%20show%20their%20importance%20in%20other%20ecosystems.%20The%20mass%20spectrometry%20and%20data%20analysis%20pipelines%20applied%20here%20offer%20a%20scalable%20framework%20for%20future%20molecular%20mapping%20and%20monitoring%20of%20marine%20ecosystems%2C%20which%20will%20contribute%20to%20a%20deliberate%20assessment%20of%20how%20chemical%20pollution%20impacts%20our%20oceans.%20%28C%29%202020%20Elsevier%20Ltd.%20All%20rights%20reserved.%22%2C%22date%22%3A%222021%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.chemosphere.2020.129450%22%2C%22ISSN%22%3A%220045-6535%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%226GX7VGSR%22%2C%22R4QPIR6K%22%2C%22QIYZ9CQ7%22%5D%2C%22dateModified%22%3A%222022-08-05T16%3A08%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22ILRNQYDP%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222021-05%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Nowak%2C%20B.%2C%20Elizur%2C%20A.%2C%20Knight%2C%20R.%2C%20Fielder%2C%20S.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282021%29.%20Impacts%20of%20the%20marine%20hatchery%20built%20environment%2C%20water%20and%20feed%20on%20across%20ontogeny%20in%20yellowtail%20kingfish%2C%20Seriola%20lalandi.%20%3Ci%3EFrontiers%20in%20Marine%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E8%3C%5C%2Fi%3E%2C%2014.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2021.676731%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2021.676731%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Impacts%20of%20the%20marine%20hatchery%20built%20environment%2C%20water%20and%20feed%20on%20across%20ontogeny%20in%20yellowtail%20kingfish%2C%20Seriola%20lalandi%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Nowak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Elizur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Fielder%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22The%20fish%20gut%20microbiome%20is%20impacted%20by%20a%20number%20of%20biological%20and%20environmental%20factors%20including%20fish%20feed%20formulations.%20Unlike%20mammals%2C%20vertical%20microbiome%20transmission%20is%20largely%20absent%20in%20fish%20and%20thus%20little%20is%20known%20about%20how%20the%20gut%20microbiome%20is%20initially%20colonized%20during%20hatchery%20rearing%20nor%20the%20stability%20throughout%20growout%20stages.%20Here%20we%20investigate%20how%20various%20microbial-rich%20surfaces%20from%20the%20built%20environment%20%26%20ldquo%3BBE%20%26%20rdquo%3B%20and%20feed%20influence%20the%20development%20of%20the%20mucosal%20microbiome%20%28gill%2C%20skin%2C%20and%20digesta%29%20of%20an%20economically%20important%20marine%20fish%2C%20yellowtail%20kingfish%2C%20Seriola%20lalandi%2C%20over%20time.%20For%20the%20first%20experiment%2C%20we%20sampled%20gill%20and%20skin%20microbiomes%20from%2036%20fish%20reared%20in%20three%20tank%20conditions%2C%20and%20demonstrate%20that%20the%20gill%20is%20more%20influenced%20by%20the%20surrounding%20environment%20than%20the%20skin.%20In%20a%20second%20experiment%2C%20fish%20mucous%20%28gill%2C%20skin%2C%20and%20digesta%29%2C%20the%20BE%20%28tank%20side%2C%20water%2C%20inlet%20pipe%2C%20airstones%2C%20and%20air%20diffusers%29%20and%20feed%20were%20sampled%20from%20indoor%20reared%20fish%20at%20three%20ages%20%2843%2C%20137%2C%20and%20430%20dph%3B%20n%20%3D%2012%20per%20age%29.%20At%20430%20dph%2C%2020%20additional%20fish%20were%20sampled%20from%20an%20outdoor%20ocean%20net%20pen.%20A%20total%20of%20304%20samples%20were%20processed%20for%2016S%20rRNA%20gene%20sequencing.%20Gill%20and%20skin%20alpha%20diversity%20increased%20while%20gut%20diversity%20decreased%20with%20age.%20Diversity%20was%20much%20lower%20in%20fish%20from%20the%20ocean%20net%20pen%20compared%20to%20indoor%20fish.%20The%20gill%20and%20skin%20are%20most%20influenced%20by%20the%20BE%20early%20in%20development%2C%20with%20aeration%20equipment%20having%20more%20impact%20in%20later%20ages%2C%20while%20the%20gut%20%26%20ldquo%3Ballochthonous%20%26%20rdquo%3B%20microbiome%20becomes%20increasingly%20differentiated%20from%20the%20environment%20over%20time.%20Feed%20had%20a%20relatively%20low%20impact%20on%20driving%20microbial%20communities.%20Our%20findings%20suggest%20that%20S.%20lalandi%20mucosal%20microbiomes%20are%20differentially%20influenced%20by%20the%20BE%20with%20a%20high%20turnover%20and%20rapid%20%26%20nbsp%3Bsuccession%20occurring%20in%20the%20gill%20and%20skin%20while%20the%20gut%20microbiome%20is%20more%20stable.%20We%20demonstrate%20how%20individual%20components%20of%20a%20hatchery%20system%2C%20especially%20aeration%20equipment%2C%20may%20contribute%20directly%20to%20microbiome%20development%20in%20a%20marine%20fish.%20In%20addition%2C%20results%20demonstrate%20how%20early%20life%20%28larval%29%20exposure%20to%20biofouling%20in%20the%20rearing%20environment%20may%20influence%20fish%20microbiome%20development%20which%20is%20important%20for%20animal%20health%20and%20aquaculture%20production.%22%2C%22date%22%3A%222021%5C%2F05%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmars.2021.676731%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A19Z%22%7D%7D%2C%7B%22key%22%3A%2256H8MRLZ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222021-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%2C%20Nowak%2C%20B.%2C%20Elizur%2C%20A.%2C%20Knight%2C%20R.%2C%20Fielder%2C%20S.%2C%20%26amp%3B%20Allen%2C%20E.%20%282021%29.%20Impacts%20of%20the%20marine%20hatchery%20built%20environment%20on%20mucosal%20microbiome%20colonization%20across%20ontogeny%20in%20yellowtail%20kingfish%2C%20Seriola%20Lalandi.%20%3Ci%3EResearch%20Square%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21203%5C%2Frs.3.rs-75521%5C%2Fv1%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21203%5C%2Frs.3.rs-75521%5C%2Fv1%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Impacts%20of%20the%20marine%20hatchery%20built%20environment%20on%20mucosal%20microbiome%20colonization%20across%20ontogeny%20in%20yellowtail%20kingfish%2C%20Seriola%20Lalandi%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Nowak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Abigail%22%2C%22lastName%22%3A%22Elizur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stewart%22%2C%22lastName%22%3A%22Fielder%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Microbial%20succession%20in%20vertebrates%20has%20primarily%20focused%20on%20vertical%20transmission%20and%20ontogenetic%20development%20in%20the%20mammalian%20gut.%20Teleosts%20comprise%20the%20majority%20of%20vertebrate%20diversity%2C%20yet%20little%20is%20known%20about%20how%20the%20microbiome%20develops%20in%20fish%2C%20particularly%20when%20vertical%20transmission%20is%20limited%20or%20absent%20for%20broadcast%20spawners.%20Biological%20factors%20such%20as%20diet%2C%20age%2C%20phylogeny%2C%20and%20trophic%20level%20along%20with%20environmental%20factors%20such%20as%20water%20salinity%2C%20temperature%2C%20and%20depth%20have%20been%20shown%20to%20influence%20the%20mucosal%20microbiomes%20of%20fish.%20Here%20we%20investigate%20how%20various%20microbial-rich%20surfaces%20from%20the%20built%20environment%20%5Cu2018BE%5Cu2019%20influence%20the%20development%20of%20the%20mucosal%20microbiome%20%28gill%2C%20skin%2C%20and%20digesta%29%20of%20an%20economically%20important%20marine%20fish%2C%20yellowtail%20kingfish%2C%20Seriola%20lalandi%2C%20over%20time.%22%2C%22date%22%3A%222021%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.21203%5C%2Frs.3.rs-75521%5C%2Fv1%22%2C%22ISSN%22%3A%222693-5015%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A19Z%22%7D%7D%2C%7B%22key%22%3A%224LPHYDKB%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nayfach%20et%20al.%22%2C%22parsedDate%22%3A%222021-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENayfach%2C%20S.%2C%20Roux%2C%20S.%2C%20Seshadri%2C%20R.%2C%20Udwary%2C%20D.%2C%20Varghese%2C%20N.%2C%20Schulz%2C%20F.%2C%20Wu%2C%20D.%2C%20Paez-Espino%2C%20D.%2C%20Chen%2C%20I.%20M.%2C%20Huntemann%2C%20M.%2C%20Palaniappan%2C%20K.%2C%20Ladau%2C%20J.%2C%20Mukherjee%2C%20S.%2C%20Reddy%2C%20T.%20B.%20K.%2C%20Nielsen%2C%20T.%2C%20Kirton%2C%20E.%2C%20Faria%2C%20J.%20P.%2C%20Edirisinghe%2C%20J.%20N.%2C%20Henry%2C%20C.%20S.%2C%20%26%23x2026%3B%20Img%20M.%20Data%20Consortium.%20%282021%29.%20A%20genomic%20catalog%20of%20Earth%26%23x2019%3Bs%20microbiomes.%20%3Ci%3ENature%20Biotechnology%3C%5C%2Fi%3E%2C%20%3Ci%3E39%3C%5C%2Fi%3E%284%29%2C%20499%26%23x2013%3B509.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41587-020-0718-6%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41587-020-0718-6%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20genomic%20catalog%20of%20Earth%5Cu2019s%20microbiomes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%22%2C%22lastName%22%3A%22Nayfach%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simon%22%2C%22lastName%22%3A%22Roux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rekha%22%2C%22lastName%22%3A%22Seshadri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Udwary%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neha%22%2C%22lastName%22%3A%22Varghese%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Frederik%22%2C%22lastName%22%3A%22Schulz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dongying%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Paez-Espino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20Min%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marcel%22%2C%22lastName%22%3A%22Huntemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Krishna%22%2C%22lastName%22%3A%22Palaniappan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joshua%22%2C%22lastName%22%3A%22Ladau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Supratim%22%2C%22lastName%22%3A%22Mukherjee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%20B.%20K.%22%2C%22lastName%22%3A%22Reddy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Torben%22%2C%22lastName%22%3A%22Nielsen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edward%22%2C%22lastName%22%3A%22Kirton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jos%5Cu00e9%20P.%22%2C%22lastName%22%3A%22Faria%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Janaka%20N.%22%2C%22lastName%22%3A%22Edirisinghe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20S.%22%2C%22lastName%22%3A%22Henry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sean%20P.%22%2C%22lastName%22%3A%22Jungbluth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dylan%22%2C%22lastName%22%3A%22Chivian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paramvir%22%2C%22lastName%22%3A%22Dehal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisha%20M.%22%2C%22lastName%22%3A%22Wood-Charlson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adam%20P.%22%2C%22lastName%22%3A%22Arkin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Susannah%20G.%22%2C%22lastName%22%3A%22Tringe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Axel%22%2C%22lastName%22%3A%22Visel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helena%22%2C%22lastName%22%3A%22Abreu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Silvia%20G.%22%2C%22lastName%22%3A%22Acinas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michelle%20A.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lauren%20V.%22%2C%22lastName%22%3A%22Alteio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gary%22%2C%22lastName%22%3A%22Andersen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%20M.%22%2C%22lastName%22%3A%22Anesio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Graeme%22%2C%22lastName%22%3A%22Attwood%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viridiana%22%2C%22lastName%22%3A%22Avila-Maga%5Cu00f1a%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yacine%22%2C%22lastName%22%3A%22Badis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jake%22%2C%22lastName%22%3A%22Bailey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brett%22%2C%22lastName%22%3A%22Baker%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Baldrian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hazel%20A.%22%2C%22lastName%22%3A%22Barton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20A.%20C.%22%2C%22lastName%22%3A%22Beck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20D.%22%2C%22lastName%22%3A%22Becraft%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harry%20R.%22%2C%22lastName%22%3A%22Beller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20Michael%22%2C%22lastName%22%3A%22Beman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rizlan%22%2C%22lastName%22%3A%22Bernier-Latmani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Timothy%20D.%22%2C%22lastName%22%3A%22Berry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%22%2C%22lastName%22%3A%22Bertagnolli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefan%22%2C%22lastName%22%3A%22Bertilsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%20M.%22%2C%22lastName%22%3A%22Bhatnagar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jordan%20T.%22%2C%22lastName%22%3A%22Bird%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20L.%22%2C%22lastName%22%3A%22Blanchard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sara%20E.%22%2C%22lastName%22%3A%22Blumer-Schuette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brendan%22%2C%22lastName%22%3A%22Bohannan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mikayla%20A.%22%2C%22lastName%22%3A%22Borton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Allyson%22%2C%22lastName%22%3A%22Brady%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Susan%20H.%22%2C%22lastName%22%3A%22Brawley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juliet%22%2C%22lastName%22%3A%22Brodie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steven%22%2C%22lastName%22%3A%22Brown%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%20R.%22%2C%22lastName%22%3A%22Brum%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%22%2C%22lastName%22%3A%22Brune%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Donald%20A.%22%2C%22lastName%22%3A%22Bryant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alison%22%2C%22lastName%22%3A%22Buchan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%20H.%22%2C%22lastName%22%3A%22Buckley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joy%22%2C%22lastName%22%3A%22Buongiorno%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hinsby%22%2C%22lastName%22%3A%22Cadillo-Quiroz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sean%20M.%22%2C%22lastName%22%3A%22Caffrey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ashley%20N.%22%2C%22lastName%22%3A%22Campbell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Campbell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephanie%22%2C%22lastName%22%3A%22Carr%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22JoLynn%22%2C%22lastName%22%3A%22Carroll%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20Craig%22%2C%22lastName%22%3A%22Cary%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%20M.%22%2C%22lastName%22%3A%22Cates%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rose%20Ann%22%2C%22lastName%22%3A%22Cattolico%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ricardo%22%2C%22lastName%22%3A%22Cavicchioli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ludmila%22%2C%22lastName%22%3A%22Chistoserdova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maureen%20L.%22%2C%22lastName%22%3A%22Coleman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Constant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%20M.%22%2C%22lastName%22%3A%22Conway%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Walter%20P.%22%2C%22lastName%22%3A%22Mac%20Cormack%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sean%22%2C%22lastName%22%3A%22Crowe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Byron%22%2C%22lastName%22%3A%22Crump%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cameron%22%2C%22lastName%22%3A%22Currie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%22%2C%22lastName%22%3A%22Daly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kristen%20M.%22%2C%22lastName%22%3A%22DeAngelis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vincent%22%2C%22lastName%22%3A%22Denef%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stuart%20E.%22%2C%22lastName%22%3A%22Denman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adey%22%2C%22lastName%22%3A%22Desta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hebe%22%2C%22lastName%22%3A%22Dionisi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremy%22%2C%22lastName%22%3A%22Dodsworth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nina%22%2C%22lastName%22%3A%22Dombrowski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Timothy%22%2C%22lastName%22%3A%22Donohue%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mark%22%2C%22lastName%22%3A%22Dopson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Timothy%22%2C%22lastName%22%3A%22Driscoll%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Dunfield%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Dynarski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginia%22%2C%22lastName%22%3A%22Edgcomb%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%20A.%22%2C%22lastName%22%3A%22Edwards%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mostafa%20S.%22%2C%22lastName%22%3A%22Elshahed%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Israel%22%2C%22lastName%22%3A%22Figueroa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beverly%22%2C%22lastName%22%3A%22Flood%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathaniel%22%2C%22lastName%22%3A%22Fortney%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%20S.%22%2C%22lastName%22%3A%22Fortunato%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Francis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%20M.%20M.%22%2C%22lastName%22%3A%22Gachon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarahi%20L.%22%2C%22lastName%22%3A%22Garcia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20C.%22%2C%22lastName%22%3A%22Gazitua%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Terry%22%2C%22lastName%22%3A%22Gentry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lena%22%2C%22lastName%22%3A%22Gerwick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Javad%22%2C%22lastName%22%3A%22Gharechahi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Girguis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%22%2C%22lastName%22%3A%22Gladden%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mary%22%2C%22lastName%22%3A%22Gradoville%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%20E.%22%2C%22lastName%22%3A%22Grasby%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kelly%22%2C%22lastName%22%3A%22Gravuer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christen%20L.%22%2C%22lastName%22%3A%22Grettenberger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20J.%22%2C%22lastName%22%3A%22Gruninger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiarong%22%2C%22lastName%22%3A%22Guo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mussie%20Y.%22%2C%22lastName%22%3A%22Habteselassie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steven%20J.%22%2C%22lastName%22%3A%22Hallam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roland%22%2C%22lastName%22%3A%22Hatzenpichler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bela%22%2C%22lastName%22%3A%22Hausmann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Terry%20C.%22%2C%22lastName%22%3A%22Hazen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brian%22%2C%22lastName%22%3A%22Hedlund%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cynthia%22%2C%22lastName%22%3A%22Henny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lydie%22%2C%22lastName%22%3A%22Herfort%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Hernandez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivia%20S.%22%2C%22lastName%22%3A%22Hershey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthias%22%2C%22lastName%22%3A%22Hess%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%20B.%22%2C%22lastName%22%3A%22Hollister%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20A.%22%2C%22lastName%22%3A%22Hug%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dana%22%2C%22lastName%22%3A%22Hunt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Janet%22%2C%22lastName%22%3A%22Jansson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Jarett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vitaly%20V.%22%2C%22lastName%22%3A%22Kadnikov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlene%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cheryl%20A.%22%2C%22lastName%22%3A%22Kerfeld%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeff%22%2C%22lastName%22%3A%22Kimbrel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%20L.%22%2C%22lastName%22%3A%22Klassen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Konstantinos%20T.%22%2C%22lastName%22%3A%22Konstantinidis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20L.%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wen-Jun%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20J.%22%2C%22lastName%22%3A%22Loder%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexander%22%2C%22lastName%22%3A%22Loy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mariana%22%2C%22lastName%22%3A%22Lozada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22MacGregor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cara%22%2C%22lastName%22%3A%22Magnabosco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aline%22%2C%22lastName%22%3A%22Maria%20da%20Silva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20Michael%22%2C%22lastName%22%3A%22McKay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%22%2C%22lastName%22%3A%22McMahon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chris%20S.%22%2C%22lastName%22%3A%22McSweeney%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00f3nica%22%2C%22lastName%22%3A%22Medina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Meredith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Mizzi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Mock%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lily%22%2C%22lastName%22%3A%22Momper%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mary%20Ann%22%2C%22lastName%22%3A%22Moran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Connor%22%2C%22lastName%22%3A%22Morgan-Lang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Duane%22%2C%22lastName%22%3A%22Moser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gerard%22%2C%22lastName%22%3A%22Muyzer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Myrold%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maisie%22%2C%22lastName%22%3A%22Nash%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camilla%20L.%22%2C%22lastName%22%3A%22Nesb%5Cu00f8%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%20P.%22%2C%22lastName%22%3A%22Neumann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%20B.%22%2C%22lastName%22%3A%22Neumann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Noguera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Trent%22%2C%22lastName%22%3A%22Northen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeanette%22%2C%22lastName%22%3A%22Norton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brent%22%2C%22lastName%22%3A%22Nowinski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Klaus%22%2C%22lastName%22%3A%22N%5Cu00fcsslein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michelle%20A.%22%2C%22lastName%22%3A%22O%5Cu2019Malley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rafael%20S.%22%2C%22lastName%22%3A%22Oliveira%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valeria%22%2C%22lastName%22%3A%22Maia%20de%20Oliveira%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tullis%22%2C%22lastName%22%3A%22Onstott%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jay%22%2C%22lastName%22%3A%22Osvatic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Ouyang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Pachiadaki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacob%22%2C%22lastName%22%3A%22Parnell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laila%20P.%22%2C%22lastName%22%3A%22Partida-Martinez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kabir%20G.%22%2C%22lastName%22%3A%22Peay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dale%22%2C%22lastName%22%3A%22Pelletier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xuefeng%22%2C%22lastName%22%3A%22Peng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Pester%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Pett-Ridge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sari%22%2C%22lastName%22%3A%22Peura%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petra%22%2C%22lastName%22%3A%22Pjevac%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alvaro%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anja%22%2C%22lastName%22%3A%22Poehlein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Phillip%20B.%22%2C%22lastName%22%3A%22Pope%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nikolai%22%2C%22lastName%22%3A%22Ravin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Molly%20C.%22%2C%22lastName%22%3A%22Redmond%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%22%2C%22lastName%22%3A%22Reiss%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginia%22%2C%22lastName%22%3A%22Rich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22Rinke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jorge%20L.%20Mazza%22%2C%22lastName%22%3A%22Rodrigues%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%22%2C%22lastName%22%3A%22Rodriguez-Reillo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karen%22%2C%22lastName%22%3A%22Rossmassler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joshua%22%2C%22lastName%22%3A%22Sackett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ghasem%20Hosseini%22%2C%22lastName%22%3A%22Salekdeh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Scott%22%2C%22lastName%22%3A%22Saleska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%22%2C%22lastName%22%3A%22Scarborough%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Schachtman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20W.%22%2C%22lastName%22%3A%22Schadt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%22%2C%22lastName%22%3A%22Schrenk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexander%22%2C%22lastName%22%3A%22Sczyrba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aditi%22%2C%22lastName%22%3A%22Sengupta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joao%20C.%22%2C%22lastName%22%3A%22Setubal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ashley%22%2C%22lastName%22%3A%22Shade%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%22%2C%22lastName%22%3A%22Sharp%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20H.%22%2C%22lastName%22%3A%22Sherman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%20V.%22%2C%22lastName%22%3A%22Shubenkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabel%20Natalia%22%2C%22lastName%22%3A%22Sierra-Garcia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rachel%22%2C%22lastName%22%3A%22Simister%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Holly%22%2C%22lastName%22%3A%22Simon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sara%22%2C%22lastName%22%3A%22Sj%5Cu00f6ling%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joan%22%2C%22lastName%22%3A%22Slonczewski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rafael%20Soares%22%2C%22lastName%22%3A%22Correa%20de%20Souza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20R.%22%2C%22lastName%22%3A%22Spear%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%20C.%22%2C%22lastName%22%3A%22Stegen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ramunas%22%2C%22lastName%22%3A%22Stepanauskas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Frank%22%2C%22lastName%22%3A%22Stewart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Garret%22%2C%22lastName%22%3A%22Suen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%22%2C%22lastName%22%3A%22Sullivan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dawn%22%2C%22lastName%22%3A%22Sumner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brandon%20K.%22%2C%22lastName%22%3A%22Swan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wesley%22%2C%22lastName%22%3A%22Swingley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%22%2C%22lastName%22%3A%22Tarn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gordon%20T.%22%2C%22lastName%22%3A%22Taylor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hanno%22%2C%22lastName%22%3A%22Teeling%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Memory%22%2C%22lastName%22%3A%22Tekere%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%22%2C%22lastName%22%3A%22Teske%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Torsten%22%2C%22lastName%22%3A%22Thomas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cameron%22%2C%22lastName%22%3A%22Thrash%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%22%2C%22lastName%22%3A%22Tiedje%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%20S.%22%2C%22lastName%22%3A%22Ting%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benjamin%22%2C%22lastName%22%3A%22Tully%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gene%22%2C%22lastName%22%3A%22Tyson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Osvlado%22%2C%22lastName%22%3A%22Ulloa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20L.%22%2C%22lastName%22%3A%22Valentine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc%20W.%22%2C%22lastName%22%3A%22Van%20Goethem%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%22%2C%22lastName%22%3A%22VanderGheynst%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tobin%20J.%22%2C%22lastName%22%3A%22Verbeke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%22%2C%22lastName%22%3A%22Vollmers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aur%5Cu00e8le%22%2C%22lastName%22%3A%22Vuillemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicholas%20B.%22%2C%22lastName%22%3A%22Waldo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20A.%22%2C%22lastName%22%3A%22Walsh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bart%20C.%22%2C%22lastName%22%3A%22Weimer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thea%22%2C%22lastName%22%3A%22Whitman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%22%2C%22lastName%22%3A%22van%20der%20Wielen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Wilkins%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Timothy%20J.%22%2C%22lastName%22%3A%22Williams%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ben%22%2C%22lastName%22%3A%22Woodcroft%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jamie%22%2C%22lastName%22%3A%22Woolet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kelly%22%2C%22lastName%22%3A%22Wrighton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jun%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erica%20B.%22%2C%22lastName%22%3A%22Young%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Noha%20H.%22%2C%22lastName%22%3A%22Youssef%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Feiqiao%20Brian%22%2C%22lastName%22%3A%22Yu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tamara%20I.%22%2C%22lastName%22%3A%22Zemskaya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ryan%22%2C%22lastName%22%3A%22Ziels%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tanja%22%2C%22lastName%22%3A%22Woyke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nigel%20J.%22%2C%22lastName%22%3A%22Mouncey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalia%20N.%22%2C%22lastName%22%3A%22Ivanova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nikos%20C.%22%2C%22lastName%22%3A%22Kyrpides%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emiley%20A.%22%2C%22lastName%22%3A%22Eloe-Fadrosh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22%22%2C%22lastName%22%3A%22Img%20M.%20Data%20Consortium%22%7D%5D%2C%22abstractNote%22%3A%22The%20reconstruction%20of%20bacterial%20and%20archaeal%20genomes%20from%20shotgun%20metagenomes%20has%20enabled%20insights%20into%20the%20ecology%20and%20evolution%20of%20environmental%20and%20host-associated%20microbiomes.%20Here%20we%20applied%20this%20approach%20to%20%3E10%2C000%20metagenomes%20collected%20from%20diverse%20habitats%20covering%20all%20of%20Earth%5Cu2019s%20continents%20and%20oceans%2C%20including%20metagenomes%20from%20human%20and%20animal%20hosts%2C%20engineered%20environments%2C%20and%20natural%20and%20agricultural%20soils%2C%20to%20capture%20extant%20microbial%2C%20metabolic%20and%20functional%20potential.%20This%20comprehensive%20catalog%20includes%2052%2C515%20metagenome-assembled%20genomes%20representing%2012%2C556%20novel%20candidate%20species-level%20operational%20taxonomic%20units%20spanning%20135%20phyla.%20The%20catalog%20expands%20the%20known%20phylogenetic%20diversity%20of%20bacteria%20and%20archaea%20by%2044%25%20and%20is%20broadly%20available%20for%20streamlined%20comparative%20analyses%2C%20interactive%20exploration%2C%20metabolic%20modeling%20and%20bulk%20download.%20We%20demonstrate%20the%20utility%20of%20this%20collection%20for%20understanding%20secondary-metabolite%20biosynthetic%20potential%20and%20for%20resolving%20thousands%20of%20new%20host%20linkages%20to%20uncultivated%20viruses.%20This%20resource%20underscores%20the%20value%20of%20genome-centric%20approaches%20for%20revealing%20genomic%20properties%20of%20uncultivated%20microorganisms%20that%20affect%20ecosystem%20processes.%22%2C%22date%22%3A%222021%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41587-020-0718-6%22%2C%22ISSN%22%3A%221546-1696%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A19Z%22%7D%7D%2C%7B%22key%22%3A%22HTIEJIYD%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222021-01%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Ali%2C%20F.%2C%20Marotz%2C%20C.%2C%20Belda-Ferre%2C%20P.%2C%20Chiang%2C%20L.%2C%20Shaffer%2C%20J.%20P.%2C%20Carpenter%2C%20C.%20S.%2C%20McDonald%2C%20D.%2C%20Gilbert%2C%20J.%2C%20Allard%2C%20S.%20M.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Knight%2C%20R.%2C%20Sweeney%2C%20D.%20A.%2C%20%26amp%3B%20Swafford%2C%20A.%20D.%20%282021%29.%20Feasibility%20of%20using%20alternative%20swabs%20and%20storage%20solutions%20for%20paired%20SARS-CoV-2%20detection%20and%20microbiome%20analysis%20in%20the%20hospital%20environment.%20%3Ci%3EMicrobiome%3C%5C%2Fi%3E%2C%20%3Ci%3E9%3C%5C%2Fi%3E%281%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs40168-020-00960-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs40168-020-00960-4%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Feasibility%20of%20using%20alternative%20swabs%20and%20storage%20solutions%20for%20paired%20SARS-CoV-2%20detection%20and%20microbiome%20analysis%20in%20the%20hospital%20environment%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Ali%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Marotz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Belda-Ferre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Chiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20P.%22%2C%22lastName%22%3A%22Shaffer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20S.%22%2C%22lastName%22%3A%22Carpenter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22McDonald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Gilbert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20M.%22%2C%22lastName%22%3A%22Allard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20A.%22%2C%22lastName%22%3A%22Sweeney%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20D.%22%2C%22lastName%22%3A%22Swafford%22%7D%5D%2C%22abstractNote%22%3A%22Determining%20the%20role%20of%20fomites%20in%20the%20transmission%20of%20SARS-CoV-2%20is%20essential%20in%20the%20hospital%20setting%20and%20will%20likely%20be%20important%20outside%20of%20medical%20facilities%20as%20governments%20around%20the%20world%20make%20plans%20to%20ease%20COVID-19%20public%20health%20restrictions%20and%20attempt%20to%20safely%20reopen%20economies.%20Expanding%20COVID-19%20testing%20to%20include%20environmental%20surfaces%20would%20ideally%20be%20performed%20with%20inexpensive%20swabs%20that%20could%20be%20transported%20safely%20without%20concern%20of%20being%20a%20source%20of%20new%20infections.%20However%2C%20CDC-approved%20clinical-grade%20sampling%20supplies%20and%20techniques%20using%20a%20synthetic%20swab%20are%20expensive%2C%20potentially%20expose%20laboratory%20workers%20to%20viable%20virus%20and%20prohibit%20analysis%20of%20the%20microbiome%20due%20to%20the%20presence%20of%20antibiotics%20in%20viral%20transport%20media%20%28VTM%29.%20To%20this%20end%2C%20we%20performed%20a%20series%20of%20experiments%20comparing%20the%20diagnostic%20yield%20using%20five%20consumer-grade%20swabs%20%28including%20plastic%20and%20wood%20shafts%20and%20various%20head%20materials%20including%20cotton%2C%20synthetic%2C%20and%20foam%29%20and%20one%20clinical-grade%20swab%20for%20inhibition%20to%20RNA.%20For%20three%20of%20these%20swabs%2C%20we%20evaluated%20performance%20to%20detect%20SARS-CoV-2%20in%20twenty%20intensive%20care%20unit%20%28ICU%29%20hospital%20rooms%20of%20patients%20including%20COVID-19%2B%20patients.%20All%20swabs%20were%20placed%20in%2095%25%20ethanol%20and%20further%20evaluated%20in%20terms%20of%20RNase%20activity.%20SARS-CoV-2%20was%20measured%20both%20directly%20from%20the%20swab%20and%20from%20the%20swab%20eluent.ResultsCompared%20to%20samples%20collected%20in%20VTM%2C%2095%25%20ethanol%20demonstrated%20significant%20inhibition%20properties%20against%20RNases.%20When%20extracting%20directly%20from%20the%20swab%20head%20as%20opposed%20to%20the%20eluent%2C%20RNA%20recovery%20was%20approximately%202-4x%20higher%20from%20all%20six%20swab%20types%20tested%20as%20compared%20to%20the%20clinical%20standard%20of%20testing%20the%20eluent%20from%20a%20CDC-approved%20synthetic%20%28SYN%29%20swab.%20The%20limit%20of%20detection%20%28LoD%29%20of%20SARS-CoV-2%20from%20floor%20samples%20collected%20using%20the%20consumer-grade%20plastic%20%28CGp%29%20or%20research-grade%20plastic%20The%20Microsetta%20Initiative%20%28TMI%29%20swabs%20was%20similar%20or%20better%20than%20the%20SYN%20swab%2C%20further%20suggesting%20that%20swab%20type%20does%20not%20impact%20RNA%20recovery%20as%20measured%20by%20the%20abundance%20of%20SARS-CoV-2.%20The%20LoD%20for%20TMI%20was%20between%200%20and%20362.5%20viral%20particles%2C%20while%20SYN%20and%20CGp%20were%20both%20between%20725%20and%201450%20particles.%20Lastly%20microbiome%20analyses%20%2816S%20rRNA%20gene%20sequencing%29%20of%20paired%20samples%20%28nasal%20and%20floor%20from%20same%20patient%20room%29%20collected%20using%20different%20swab%20types%20in%20triplicate%20indicated%20that%20microbial%20communities%20were%20not%20impacted%20by%20swab%20type%2C%20but%20instead%20driven%20by%20the%20patient%20and%20sample%20type.ConclusionsCompared%20to%20using%20a%20clinical-grade%20synthetic%20swab%2C%20detection%20of%20SARS-CoV-2%20from%20environmental%20samples%20collected%20from%20ICU%20rooms%20of%20patients%20with%20COVID%20was%20similar%20using%20consumer-grade%20swabs%2C%20stored%20in%2095%25%20ethanol.%20The%20yield%20was%20best%20from%20the%20swab%20head%20rather%20than%20the%20eluent%20and%20the%20low%20level%20of%20RNase%20activity%20and%20lack%20of%20antibiotics%20in%20these%20samples%20makes%20it%20possible%20to%20perform%20concomitant%20microbiome%20analyses.%22%2C%22date%22%3A%222021%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs40168-020-00960-4%22%2C%22ISSN%22%3A%222049-2618%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2228H8GH9D%22%5D%2C%22dateModified%22%3A%222022-08-15T16%3A14%3A59Z%22%7D%7D%2C%7B%22key%22%3A%22YEY67VRC%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Neu%20et%20al.%22%2C%22parsedDate%22%3A%222021-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENeu%2C%20A.%20T.%2C%20Hughes%2C%20I.%20V.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Roy%2C%20K.%20%282021%29.%20Decade-scale%20stability%20and%20change%20in%20a%20marine%20bivalve%20microbiome.%20%3Ci%3EMolecular%20Ecology%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15796%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15796%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Decade-scale%20stability%20and%20change%20in%20a%20marine%20bivalve%20microbiome%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20T.%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20V.%22%2C%22lastName%22%3A%22Hughes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Roy%22%7D%5D%2C%22abstractNote%22%3A%22Predicting%20how%20populations%20and%20communities%20of%20organisms%20will%20respond%20to%20anthropogenic%20change%20is%20of%20paramount%20concern%20in%20ecology%20today.%20For%20communities%20of%20microorganisms%2C%20however%2C%20these%20predictions%20remain%20challenging%2C%20primarily%20due%20to%20data%20limitations.%20Information%20about%20long-term%20dynamics%20of%20host-associated%20microbial%20communities%2C%20in%20particular%2C%20is%20lacking.%20In%20this%20study%2C%20we%20use%20well-preserved%20and%20freshly%20collected%20samples%20of%20soft%20tissue%20from%20a%20marine%20bivalve%20host%2C%20Donax%20gouldii%2C%20at%20a%20single%20site%20to%20quantify%20the%20diversity%20and%20composition%20of%20its%20microbiome%20over%20a%20decadal%20timescale.%20Site-level%20measurements%20of%20temperature%2C%20salinity%20and%20chlorophyll%20a%20allowed%20us%20to%20test%20how%20the%20microbiome%20of%20this%20species%20responded%20to%20two%20natural%20experiments%3A%20a%20seasonal%20increase%20in%20temperature%20and%20a%20phytoplankton%20bloom.%20Our%20results%20show%20that%20ethanol-preserved%20tissue%20can%20provide%20high-resolution%20information%20about%20temporal%20trends%20in%20compositions%20of%20host-associated%20microbial%20communities.%20Specifically%2C%20we%20found%20that%20the%20richness%20of%20amplicon%20sequence%20variants%20%28ASVs%29%20associated%20with%20D.gouldii%20did%20not%20change%20significantly%20over%20time%20despite%20increases%20in%20water%20temperature%20%28%2B1.6%20degrees%20C%20due%20to%20seasonal%20change%29%20and%20chlorophyll%20a%20concentration%20%28more%20than%20ninefold%29.%20The%20phylogenetic%20composition%20of%20the%20communities%2C%20on%20the%20other%20hand%2C%20varied%20significantly%20between%20all%20collection%20years%2C%20with%20only%20six%20ASVs%20persisting%20over%20our%20sampling%20period.%20Overall%2C%20these%20results%20suggest%20that%20the%20diversity%20of%20microbial%20taxa%20associated%20with%20D.gouldii%20has%20remained%20stable%20over%20time%20and%20in%20response%20to%20seasonal%20environmental%20change%20over%20the%20course%20of%20more%20than%20a%20decade%2C%20but%20such%20stability%20is%20underlain%20by%20substantial%20turnover%20in%20the%20composition%20of%20the%20microbiome.%22%2C%22date%22%3A%222021%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fmec.15796%22%2C%22ISSN%22%3A%220962-1083%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A17Z%22%7D%7D%2C%7B%22key%22%3A%228DPULCG7%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222020-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Power%2C%20C.%2C%20Melanson%2C%20M.%2C%20Knight%2C%20R.%2C%20Webber%2C%20C.%2C%20Rough%2C%20K.%2C%20Bott%2C%20N.%20J.%2C%20Nowak%2C%20B.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282020%29.%20The%20Southern%20Bluefin%20Tuna%20mucosal%20microbiome%20is%20influenced%20by%20husbandry%20method%2C%20net%20pen%20location%2C%20and%20anti-parasite%20treatment.%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2F2020.05.19.105270%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2F2020.05.19.105270%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Southern%20Bluefin%20Tuna%20mucosal%20microbiome%20is%20influenced%20by%20husbandry%20method%2C%20net%20pen%20location%2C%20and%20anti-parasite%20treatment%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cecilia%22%2C%22lastName%22%3A%22Power%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michaela%22%2C%22lastName%22%3A%22Melanson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Webber%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kirsten%22%2C%22lastName%22%3A%22Rough%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathan%20J.%22%2C%22lastName%22%3A%22Bott%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Nowak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Aquaculture%20is%20the%20fastest%20growing%20primary%20industry%20worldwide.%20Marine%20finfish%20culture%20in%20open%20ocean%20net%20pens%2C%20or%20pontoons%2C%20is%20one%20of%20the%20largest%20growth%20areas%20and%20is%20currently%20the%20only%20way%20to%20rear%20high%20value%20fish%20such%20as%20bluefin%20tuna.%20Ranching%20involves%20catching%20wild%20juveniles%2C%20stocking%20in%20floating%20net%20pens%20and%20fattening%20for%20four%20to%20eight%20months.%20Tuna%20experience%20several%20parasite-induced%20disease%20challenges%20in%20culture%20that%20can%20be%20mitigated%20by%20application%20of%20praziquantel%20%28PZQ%29%20as%20a%20therapeutic.%20In%20this%20study%2C%20we%20characterized%20the%20microbiome%20of%20ranched%20southern%20Bluefin%20Tuna%2C%20Thunnus%20maccoyii%2C%20across%20four%20anatomic%20sites%20%28gill%2C%20skin%2C%20digesta%2C%20and%20anterior%20kidney%29%20and%20evaluated%20environmental%20and%20pathological%20factors%20that%20influence%20microbiome%20composition%2C%20including%20the%20impact%20of%20PZQ%20treatment%20on%20microbiome%20stability.%20Southern%20bluefin%20tuna%20gill%2C%20skin%2C%20and%20digesta%20microbiome%20communities%20are%20unique%20and%20potentially%20influenced%20by%20husbandry%20practices%2C%20location%20of%20pontoon%20growout%20pens%2C%20and%20treatment%20with%20the%20antiparasitic%20PZQ.%20There%20was%20no%20significant%20relationship%20between%20the%20fish%20mucosal%20microbiome%20and%20incidence%20or%20abundance%20of%20adult%20blood%20fluke%20in%20the%20heart%20or%20fluke%20egg%20density%20in%20the%20gill.%20An%20enhanced%20understanding%20of%20microbiome%20diversity%20and%20function%20in%20high-value%20farmed%20fish%20species%20such%20as%20bluefin%20tuna%20is%20needed%20to%20optimize%20fish%20health%20and%20improve%20aquaculture%20yield.%20Comparison%20of%20the%20bluefin%20tuna%20microbiome%20to%20other%20fish%20species%2C%20including%20Seriola%20lalandi%20%28yellowtail%20kingfish%29%2C%20a%20common%20farmed%20species%20from%20Australia%2C%20and%20Scomber%20japonicus%20%28Pacific%20mackerel%29%2C%20a%20wild%20caught%20Scombrid%20relative%20of%20tuna%2C%20showed%20the%20two%20Scombrids%20had%20more%20similar%20microbial%20communities%20compared%20to%20other%20families.%20The%20finding%20that%20mucosal%20microbial%20communities%20are%20more%20similar%20in%20phylogenetically%20related%20fish%20species%20exposes%20an%20opportunity%20to%20develop%20mackerel%20as%20a%20model%20for%20tuna%20microbiome%20and%20parasite%20research.%22%2C%22date%22%3A%222020%5C%2F08%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1101%5C%2F2020.05.19.105270%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A21Z%22%7D%7D%2C%7B%22key%22%3A%22DCNXYADL%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Podell%20et%20al.%22%2C%22parsedDate%22%3A%222020-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPodell%2C%20S.%2C%20Blanton%2C%20J.%20M.%2C%20Oliver%2C%20A.%2C%20Schorn%2C%20M.%20A.%2C%20Agarwal%2C%20V.%2C%20Biggs%2C%20J.%20S.%2C%20Moore%2C%20B.%20S.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282020%29.%20A%20genomic%20view%20of%20trophic%20and%20metabolic%20diversity%20in%20clade-specific%20Lamellodysidea%20sponge%20microbiomes.%20%3Ci%3EMicrobiome%3C%5C%2Fi%3E%2C%20%3Ci%3E8%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21203%5C%2Frs.2.17204%5C%2Fv1%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21203%5C%2Frs.2.17204%5C%2Fv1%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20genomic%20view%20of%20trophic%20and%20metabolic%20diversity%20in%20clade-specific%20Lamellodysidea%20sponge%20microbiomes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aaron%22%2C%22lastName%22%3A%22Oliver%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michelle%20A.%22%2C%22lastName%22%3A%22Schorn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vinayak%22%2C%22lastName%22%3A%22Agarwal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20S.%22%2C%22lastName%22%3A%22Biggs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Marine%20sponges%20and%20their%20microbiomes%20contribute%20significantly%20to%20carbon%20and%20nutrient%20cycling%20in%20global%20reefs%2C%20processing%20and%20remineralizing%20dissolved%20and%20particulate%20organic%20matter.%20Lamellodysidea%20herbacea%20sponges%20obtain%20additional%20energy%20from%20abundant%20photosynthetic%20Hormoscilla%20cyanobacterial%20symbionts%2C%20which%20also%20produce%20polybrominated%20diphenyl%20ethers%20%28PBDEs%29%20chemically%20similar%20to%20anthropogenic%20pollutants%20of%20environmental%20concern.%20Potential%20contributions%20of%20non-Hormoscilla%20bacteria%20to%20Lamellodysidea%20microbiome%20metabolism%20and%20the%20synthesis%20and%20degradation%20of%20additional%20secondary%20metabolites%20are%20currently%20unknown.%5Cn%5CnResults%3A%20This%20study%20has%20determined%20relative%20abundance%2C%20taxonomic%20novelty%2C%20metabolic%20capacities%2C%20and%20secondary%20metabolite%20potential%20in%2021%20previously%20uncharacterized%2C%20uncultured%20Lamellodysidea-associated%20microbial%20populations%20by%20reconstructing%20near-complete%20metagenome-assembled%20genomes%20%28MAGs%29%20to%20complement%2016S%20rRNA%20gene%20amplicon%20studies.%20Microbial%20community%20structures%20aligned%20with%20sponge%20host%20subgroup%20phylogeny%20in%2016%20samples%20from%20four%20host%20clades%20collected%20from%20multiple%20sites%20in%20Guam%20over%20a%20three%20year%20period%2C%20including%20representatives%20of%20Alphaproteobacteria%2C%20Gammaproteobacteria%2C%20Oligoflexia%2C%20and%20Bacteroidetes%20as%20well%20as%20Cyanobacteria%20%28Hormoscilla%29.%20Unexpectedly%2C%20microbiomes%20from%20one%20host%20clade%20also%20included%20Cyanobacteria%20from%20the%20chemically%20talented%20genus%20Prochloron%2C%20a%20common%20tunicate%20symbiont.%20Two%20novel%20Alphaprotobacteria%20MAGs%20encoded%20pathways%20diagnostic%20for%20methylotrophic%20metabolism%20as%20well%20as%20Type%20III%20secretion%20systems%2C%20and%20have%20been%20provisionally%20assigned%20to%20a%20new%20order%2C%20designated%20Candidatus%20Methylospongiales.%20MAGs%20from%20other%20taxonomic%20groups%20encoded%20light-driven%20energy%20production%20pathways%20using%20not%20only%20chlorophyll%2C%20but%20also%20bacteriochlorophyll%20and%20proteorhodopsin.%20Diverse%20heterotrophic%20capabilities%20favoring%20aerobic%20versus%20anaerobic%20conditions%20included%20pathways%20for%20degrading%20chitin%2C%20eukaryotic%20extracellular%20matrix%20polymers%2C%20phosphonates%2C%20dimethylsulfoniopropionate%2C%20trimethylamine%2C%20and%20benzoate.%20Genetic%20evidence%20identified%20an%20aerobic%20catabolic%20pathway%20for%20halogenated%20aromatics%20that%20may%20enable%20endogenous%20PBDEs%20to%20be%20used%20as%20a%20carbon%20and%20energy%20source.%20%5Cn%5CnConclusions%3A%20The%20reconstruction%20of%20high%20quality%20MAGs%20from%20all%20microbial%20taxa%20comprising%20greater%20than%200.1%25%20of%20the%20sponge%20microbiome%20enabled%20species-specific%20assignment%20of%20unique%20metabolic%20features%20that%20could%20not%20have%20been%20predicted%20from%20taxonomic%20data%20alone.%20This%20information%20will%20promote%20more%20representative%20models%20of%20marine%20invertebrate%20microbiome%20contributions%20to%20host%20bioenergetics%2C%20the%20identification%20of%20potential%20new%20sponge%20parasites%20and%20pathogens%20based%20on%20conserved%20metabolic%20and%20physiological%20markers%2C%20and%20a%20better%20understanding%20of%20biosynthetic%20and%20degradative%20pathways%20for%20secondary%20metabolites%20and%20halogenated%20compounds%20in%20sponge-associated%20microbiota.%22%2C%22date%22%3A%222020%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.21203%5C%2Frs.2.17204%5C%2Fv1%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222022-07-27T16%3A49%3A30Z%22%7D%7D%2C%7B%22key%22%3A%223JBP3TAM%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Allemann%20and%20Allen%22%2C%22parsedDate%22%3A%222020-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAllemann%2C%20M.%20N.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282020%29.%20Genetic%20regulation%20of%20the%20bacterial%20omega-3%20polyunsaturated%20fatty%20acid%20biosynthesis%20pathway.%20%3Ci%3EJournal%20of%20Bacteriology%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJB.00050-20%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJB.00050-20%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Genetic%20regulation%20of%20the%20bacterial%20omega-3%20polyunsaturated%20fatty%20acid%20biosynthesis%20pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marco%20Nicholas%22%2C%22lastName%22%3A%22Allemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22A%20characteristic%20among%20many%20marine%20Gammaproteobacteria%20is%20the%20biosynthesis%20and%20incorporation%20of%20omega-3%20polyunsaturated%20fatty%20acids%20into%20membrane%20phospholipids.%20The%20biosynthesis%20of%20eicosapentaenoic%20acid%20%28EPA%29%20and%5C%2For%20docosahexaenoic%20acid%20%28DHA%29%20is%20mediated%20by%20a%20polyketide%5C%2Ffatty%20acid%20synthase%20mechanism%20encoded%20by%20a%20set%20of%20five%20genes%2C%20pfaABCDE.%20This%20unique%20fatty%20acid%20synthesis%20pathway%20coexists%20with%20the%20principal%20type%20II%20dissociated%20fatty%20acid%20synthesis%20pathway%2C%20which%20is%20responsible%20for%20the%20biosynthesis%20of%20core%20saturated%2C%20monounsaturated%2C%20and%20hydroxylated%20fatty%20acids%20used%20in%20phospholipid%20and%20lipid%20A%20biosynthesis.%20In%20this%20work%2C%20a%20genetic%20approach%20was%20undertaken%20to%20elucidate%20genetic%20regulation%20of%20the%20pfa%20genes%20in%20the%20model%20marine%20bacterium%20Photobacterium%20profundum%20SS9.%20Using%20a%20reporter%20gene%20fusion%2C%20we%20showed%20that%20expression%20of%20the%20pfa%20operon%20is%20downregulated%20in%20response%20to%20exogenous%20fatty%20acids%2C%20particularly%20long-chain%20monounsaturated%20fatty%20acids.%20This%20regulation%20occurs%20independently%20of%20the%20canonical%20fatty%20acid%20regulators%2C%20FabR%20and%20FadR%2C%20present%20in%20P.%20profundum%20SS9.%20Transposon%20mutagenesis%20and%20screening%20of%20a%20library%20of%20mutants%20identified%20a%20novel%20transcriptional%20regulator%2C%20which%20we%20have%20designated%20pfaF%2C%20to%20be%20responsible%20for%20the%20observed%20regulation%20of%20the%20pfa%20operon%20in%20P.%20profundum%20SS9.%20Gel%20mobility%20shift%20and%20DNase%20I%20footprinting%20assays%20confirmed%20that%20PfaF%20binds%20the%20pfaA%20promoter%20and%20identified%20the%20PfaF%20binding%20site.%22%2C%22date%22%3A%222020%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FJB.00050-20%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22MJRY7WV7%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Schulhof%20et%20al.%22%2C%22parsedDate%22%3A%222020-06%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESchulhof%2C%20M.%20A.%2C%20Allen%2C%20A.%20E.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Mladenov%2C%20N.%2C%20McCrow%2C%20J.%20P.%2C%20Jones%2C%20N.%20T.%2C%20Blanton%2C%20J.%2C%20Cavalheri%2C%20H.%20B.%2C%20Kaul%2C%20D.%2C%20Symons%2C%20C.%20C.%2C%20%26amp%3B%20Shurin%2C%20J.%20B.%20%282020%29.%20Sierra%20Nevada%20mountain%20lake%20microbial%20communities%20are%20structured%20by%20temperature%2C%20resources%20and%20geographic%20location.%20%3Ci%3EMolecular%20Ecology%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15469%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15469%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Sierra%20Nevada%20mountain%20lake%20microbial%20communities%20are%20structured%20by%20temperature%2C%20resources%20and%20geographic%20location%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marika%20A.%22%2C%22lastName%22%3A%22Schulhof%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalie%22%2C%22lastName%22%3A%22Mladenov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20P.%22%2C%22lastName%22%3A%22McCrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalie%20T.%22%2C%22lastName%22%3A%22Jones%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hamanda%20B.%22%2C%22lastName%22%3A%22Cavalheri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Drishti%22%2C%22lastName%22%3A%22Kaul%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Celia%20C.%22%2C%22lastName%22%3A%22Symons%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%20B.%22%2C%22lastName%22%3A%22Shurin%22%7D%5D%2C%22abstractNote%22%3A%22Warming%2C%20eutrophication%20%28nutrient%20fertilization%29%20and%20brownification%20%28increased%20loading%20of%20allochthonous%20organic%20matter%29%20are%20three%20global%20trends%20impacting%20lake%20ecosystems.%20However%2C%20the%20independent%20and%20synergistic%20effects%20of%20resource%20addition%20and%20warming%20on%20autotrophic%20and%20heterotrophic%20microorganisms%20are%20largely%20unknown.%20In%20this%20study%2C%20we%20investigate%20the%20independent%20and%20interactive%20effects%20of%20temperature%2C%20dissolved%20organic%20carbon%20%28DOC%2C%20both%20allochthonous%20and%20autochthonous%29%20and%20nitrogen%20%28N%29%20supply%2C%20in%20addition%20to%20the%20effect%20of%20spatial%20variables%2C%20on%20the%20composition%2C%20richness%2C%20and%20evenness%20of%20prokaryotic%20and%20eukaryotic%20microbial%20communities%20in%20lakes%20across%20elevation%20and%20N%20deposition%20gradients%20in%20the%20Sierra%20Nevada%20mountains%20of%20California%2C%20USA.%20We%20found%20that%20both%20prokaryotic%20and%20eukaryotic%20communities%20are%20structured%20by%20temperature%2C%20terrestrial%20%28allochthonous%29%20DOC%20and%20latitude.%20Prokaryotic%20communities%20are%20also%20influenced%20by%20total%20and%20aquatic%20%28autochthonous%29%20DOC%2C%20while%20eukaryotic%20communities%20are%20also%20structured%20by%20nitrate.%20Additionally%2C%20increasing%20N%20availability%20was%20associated%20with%20reduced%20richness%20of%20prokaryotic%20communities%2C%20and%20both%20lower%20richness%20and%20evenness%20of%20eukaryotes.%20We%20did%20not%20detect%20any%20synergistic%20or%20antagonistic%20effects%20as%20there%20were%20no%20interactions%20among%20temperature%20and%20resource%20variables.%20Together%2C%20our%20results%20suggest%20that%20%28a%29%20organic%20and%20inorganic%20resources%2C%20temperature%2C%20and%20geographic%20location%20%28based%20on%20latitude%20and%20longitude%29%20independently%20influence%20lake%20microbial%20communities%3B%20and%20%28b%29%20increasing%20N%20supply%20due%20to%20atmospheric%20N%20deposition%20may%20reduce%20richness%20of%20both%20prokaryotic%20and%20eukaryotic%20microbes%2C%20probably%20by%20reducing%20niche%20dimensionality.%20Our%20study%20provides%20insight%20into%20abiotic%20processes%20structuring%20microbial%20communities%20across%20environmental%20gradients%20and%20their%20potential%20roles%20in%20material%20and%20energy%20fluxes%20within%20and%20between%20ecosystems.%22%2C%22date%22%3A%222020%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fmec.15469%22%2C%22ISSN%22%3A%220962-1083%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2286H9SNJB%22%5D%2C%22dateModified%22%3A%222022-08-05T16%3A15%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22H4HJXSDY%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Fedarko%20et%20al.%22%2C%22parsedDate%22%3A%222020-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFedarko%2C%20M.%20W.%2C%20Martino%2C%20C.%2C%20Morton%2C%20J.%20T.%2C%20Gonz%26%23xE1%3Blez%2C%20A.%2C%20Rahman%2C%20G.%2C%20Marotz%2C%20C.%20A.%2C%20Minich%2C%20J.%20J.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Knight%2C%20R.%20%282020%29.%20Visualizing%20%26%23x2019%3Bomic%20feature%20rankings%20and%20log-ratios%20using%20Qurro.%20%3Ci%3ENAR%20Genomics%20and%20Bioinformatics%3C%5C%2Fi%3E%2C%20%3Ci%3E2%3C%5C%2Fi%3E%282%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnargab%5C%2Flqaa023%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnargab%5C%2Flqaa023%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Visualizing%20%5Cu2019omic%20feature%20rankings%20and%20log-ratios%20using%20Qurro%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marcus%20W.%22%2C%22lastName%22%3A%22Fedarko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cameron%22%2C%22lastName%22%3A%22Martino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%20T.%22%2C%22lastName%22%3A%22Morton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonio%22%2C%22lastName%22%3A%22Gonz%5Cu00e1lez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gibraan%22%2C%22lastName%22%3A%22Rahman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Clarisse%20A.%22%2C%22lastName%22%3A%22Marotz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%5D%2C%22abstractNote%22%3A%22Many%20tools%20for%20dealing%20with%20compositional%20%5Cu2018%5Cu2009%5Cu2019omics%5Cu2019%20data%20produce%20feature-wise%20values%20that%20can%20be%20ranked%20in%20order%20to%20describe%20features%5Cu2019%20associations%20with%20some%20sort%20of%20variation.%20These%20values%20include%20differentials%20%28which%20describe%20features%5Cu2019%20associations%20with%20specified%20covariates%29%20and%20feature%20loadings%20%28which%20describe%20features%5Cu2019%20associations%20with%20variation%20along%20a%20given%20axis%20in%20a%20biplot%29.%20Although%20prior%20work%20has%20discussed%20the%20use%20of%20these%20%5Cu2018rankings%5Cu2019%20as%20a%20starting%20point%20for%20exploring%20the%20log-ratios%20of%20particularly%20high-%20or%20low-ranked%20features%2C%20such%20exploratory%20analyses%20have%20previously%20been%20done%20using%20custom%20code%20to%20visualize%20feature%20rankings%20and%20the%20log-ratios%20of%20interest.%20This%20approach%20is%20laborious%2C%20prone%20to%20errors%20and%20raises%20questions%20about%20reproducibility.%20To%20address%20these%20problems%20we%20introduce%20Qurro%2C%20a%20tool%20that%20interactively%20visualizes%20a%20plot%20of%20feature%20rankings%20%28a%20%5Cu2018rank%20plot%5Cu2019%29%20alongside%20a%20plot%20of%20selected%20features%5Cu2019%20log-ratios%20within%20samples%20%28a%20%5Cu2018sample%20plot%5Cu2019%29.%20Qurro%5Cu2019s%20interface%20includes%20various%20controls%20that%20allow%20users%20to%20select%20features%20from%20along%20the%20rank%20plot%20to%20compute%20a%20log-ratio%3B%20this%20action%20updates%20both%20the%20rank%20plot%20%28through%20highlighting%20selected%20features%29%20and%20the%20sample%20plot%20%28through%20displaying%20the%20current%20log-ratios%20of%20samples%29.%20Here%2C%20we%20demonstrate%20how%20this%20unique%20interface%20helps%20users%20explore%20feature%20rankings%20and%20log-ratios%20simply%20and%20effectively.%22%2C%22date%22%3A%222020%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fnargab%5C%2Flqaa023%22%2C%22ISSN%22%3A%222631-9268%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A21Z%22%7D%7D%2C%7B%22key%22%3A%22DTPJ7RKK%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222020-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%2C%20Petrus%2C%20S.%2C%20Michael%2C%20J.%20D.%2C%20Michael%2C%20T.%20P.%2C%20Knight%2C%20R.%2C%20%26amp%3B%20Allen%2C%20E.%20%282020%29.%20Temporal%2C%20environmental%2C%20and%20biological%20drivers%20of%20the%20mucosal%20microbiome%20in%20a%20wild%20marine%20fish%2C%20Scomber%20japonicus.%20%3Ci%3EMSphere%3C%5C%2Fi%3E%2C%20%3Ci%3E5%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSphere.00401-20%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSphere.00401-20%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Temporal%2C%20environmental%2C%20and%20biological%20drivers%20of%20the%20mucosal%20microbiome%20in%20a%20wild%20marine%20fish%2C%20Scomber%20japonicus%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Semar%22%2C%22lastName%22%3A%22Petrus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julius%20D.%22%2C%22lastName%22%3A%22Michael%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Todd%20P.%22%2C%22lastName%22%3A%22Michael%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Changing%20ocean%20conditions%20driven%20by%20anthropogenic%20activity%20may%20have%20a%20negative%20impact%20on%20fisheries%20by%20increasing%20stress%20and%20disease%20with%20the%20mucosal%20microbiome%20as%20a%20potentially%20important%20intermediate%20role.%20To%20understand%20how%20environment%20and%20host%20biology%20drives%20mucosal%20microbiomes%20in%20a%20marine%20fish%2C%20we%20surveyed%20five%20body%20sites%20%28gill%2C%20skin%2C%20digesta%2C%20GI%2C%20and%20pyloric%20caeca%29%20from%20229%20Pacific%20chub%20mackerel%2C%20Scomber%20japonicus%2C%20collected%20across%2038%20time%20points%20spanning%20one%20year%20from%20the%20Scripps%20Institution%20of%20Oceanography%20Pier%2C%20making%20this%20the%20largest%20and%20longest%20wild%20marine%20fish%20microbiome%20survey.%20Mucosal%20sites%20had%20unique%20communities%20significantly%20different%20from%20the%20surrounding%20sea%20water%20and%20sediment%20communities%20with%20over%2010%20times%20more%20diversity%20than%20sea%20water%20alone.%20Although%2C%20external%20surfaces%20such%20as%20skin%20and%20gill%20were%20more%20similar%20to%20sea%20water%2C%20digesta%20was%20similar%20to%20sediment.%20Both%20alpha%20and%20beta%20diversity%20of%20the%20skin%20and%20gill%20was%20explained%20by%20environmental%20and%20biological%20factors%2C%20especially%20sea%20surface%20temperature%2C%20chlorophyll%20a%2C%20and%20fish%20age%2C%20consistent%20with%20an%20exposure%20gradient%20relationship.%20We%20verified%20that%20seasonal%20microbial%20changes%20were%20not%20confounded%20by%20migrations%20of%20chub%20mackerel%20sub-populations%20by%20nanopore%20sequencing%20a%2014%20769%20bp%20region%20of%20the%2016%20568%20bp%20mitochondria.%20A%20cosmopolitan%20pathogen%2C%20Photobacterium%20damselae%2C%20was%20prevalent%20across%20multiple%20body%20sites%20all%20year%2C%20but%20highest%20in%20the%20skin%2C%20GI%2C%20and%20digesta%20between%20June%20and%20September.%20Our%20study%20evaluates%20the%20extent%20which%20the%20environment%20and%20host%20biology%20drives%20mucosal%20microbial%20ecology%2C%20establishing%20a%20baseline%20for%20long%20term%20monitoring%20surveys%20for%20linking%20environment%20stressors%20to%20mucosal%20health%20of%20wild%20marine%20fish.%22%2C%22date%22%3A%222020%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmSphere.00401-20%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A21Z%22%7D%7D%2C%7B%22key%22%3A%224ES5EAH2%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mohanty%20et%20al.%22%2C%22parsedDate%22%3A%222020-02%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMohanty%2C%20I.%2C%20Podell%2C%20S.%2C%20Biggs%2C%20J.%20S.%2C%20Garg%2C%20N.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Agarwal%2C%20V.%20%282020%29.%20Multi-omic%20profiling%20of%20melophlus%20sponges%20reveals%20diverse%20metabolomic%20and%20microbiome%20architectures%20that%20are%20non-overlapping%20with%20ecological%20neighbors.%20%3Ci%3EMarine%20Drugs%3C%5C%2Fi%3E%2C%20%3Ci%3E18%3C%5C%2Fi%3E%282%29%2C%20124.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fmd18020124%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fmd18020124%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Multi-omic%20profiling%20of%20melophlus%20sponges%20reveals%20diverse%20metabolomic%20and%20microbiome%20architectures%20that%20are%20non-overlapping%20with%20ecological%20neighbors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ipsita%22%2C%22lastName%22%3A%22Mohanty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20S.%22%2C%22lastName%22%3A%22Biggs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neha%22%2C%22lastName%22%3A%22Garg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vinayak%22%2C%22lastName%22%3A%22Agarwal%22%7D%5D%2C%22abstractNote%22%3A%22Marine%20sponge%20holobionts%2C%20defined%20as%20filter-feeding%20sponge%20hosts%20together%20with%20their%20associated%20microbiomes%2C%20are%20prolific%20sources%20of%20natural%20products.%20The%20inventory%20of%20natural%20products%20that%20have%20been%20isolated%20from%20marine%20sponges%20is%20extensive.%20Here%2C%20using%20untargeted%20mass%20spectrometry%2C%20we%20demonstrate%20that%20sponges%20harbor%20a%20far%20greater%20diversity%20of%20low-abundance%20natural%20products%20that%20have%20evaded%20discovery.%20While%20these%20low-abundance%20natural%20products%20may%20not%20be%20feasible%20to%20isolate%2C%20insights%20into%20their%20chemical%20structures%20can%20be%20gleaned%20by%20careful%20curation%20of%20mass%20fragmentation%20spectra.%20Sponges%20are%20also%20some%20of%20the%20most%20complex%2C%20multi-organismal%20holobiont%20communities%20in%20the%20oceans.%20We%20overlay%20sponge%20metabolomes%20with%20their%20microbiome%20structures%20and%20detailed%20metagenomic%20characterization%20to%20discover%20candidate%20gene%20clusters%20that%20encode%20production%20of%20sponge-derived%20natural%20products.%20The%20multi-omic%20profiling%20strategy%20for%20sponges%20that%20we%20describe%20here%20enables%20quantitative%20comparison%20of%20sponge%20metabolomes%20and%20microbiomes%20to%20address%2C%20among%20other%20questions%2C%20the%20ecological%20relevance%20of%20sponge%20natural%20products%20and%20for%20the%20phylochemical%20assignment%20of%20previously%20undescribed%20sponge%20identities.%22%2C%22date%22%3A%222020%5C%2F02%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fmd18020124%22%2C%22ISSN%22%3A%221660-3397%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22R3FMCBUI%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kolody%20et%20al.%22%2C%22parsedDate%22%3A%222019-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKolody%2C%20B.%20C.%2C%20McCrow%2C%20J.%20P.%2C%20Allen%2C%20L.%20Z.%2C%20Aylward%2C%20F.%20O.%2C%20Fontanez%2C%20K.%20M.%2C%20Moustafa%2C%20A.%2C%20Moniruzzaman%2C%20M.%2C%20Chavez%2C%20F.%20P.%2C%20Scholin%2C%20C.%20A.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Worden%2C%20A.%20Z.%2C%20Delong%2C%20E.%20F.%2C%20%26amp%3B%20Allen%2C%20A.%20E.%20%282019%29.%20Diel%20transcriptional%20response%20of%20a%20California%20Current%20plankton%20microbiome%20to%20light%2C%20low%20iron%2C%20and%20enduring%20viral%20infection.%20%3Ci%3EThe%20ISME%20Journal%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-019-0472-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-019-0472-2%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Diel%20transcriptional%20response%20of%20a%20California%20Current%20plankton%20microbiome%20to%20light%2C%20low%20iron%2C%20and%20enduring%20viral%20infection%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20C.%22%2C%22lastName%22%3A%22Kolody%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20P.%22%2C%22lastName%22%3A%22McCrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20Zeigler%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%20O.%22%2C%22lastName%22%3A%22Aylward%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20M.%22%2C%22lastName%22%3A%22Fontanez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Moustafa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Moniruzzaman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%20P.%22%2C%22lastName%22%3A%22Chavez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20A.%22%2C%22lastName%22%3A%22Scholin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20Z.%22%2C%22lastName%22%3A%22Worden%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20F.%22%2C%22lastName%22%3A%22Delong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Phytoplankton%20and%20associated%20microbial%20communities%20provide%20organic%20carbon%20to%20oceanic%20food%20webs%20and%20drive%20ecosystem%20dynamics.%20However%2C%20capturing%20those%20dynamics%20is%20challenging.%20Here%2C%20an%20in%20situ%2C%20semi-Lagrangian%2C%20robotic%20sampler%20profiled%20pelagic%20microbes%20at%204%5Cu2009h%20intervals%20over%20~2.6%20days%20in%20North%20Pacific%20high-nutrient%2C%20low-chlorophyll%20waters.%20We%20report%20on%20the%20community%20structure%20and%20transcriptional%20dynamics%20of%20microbes%20in%20an%20operationally%20large%20size%20class%20%28%3E5%5Cu2009%5Cu03bcm%29%20predominantly%20populated%20by%20dinoflagellates%2C%20ciliates%2C%20haptophytes%2C%20pelagophytes%2C%20diatoms%2C%20cyanobacteria%20%28chiefly%20Synechococcus%29%2C%20prasinophytes%20%28chiefly%20Ostreococcus%29%2C%20fungi%2C%20archaea%2C%20and%20proteobacteria.%20Apart%20from%20fungi%20and%20archaea%2C%20all%20groups%20exhibited%2024-h%20periodicity%20in%20some%20transcripts%2C%20but%20larger%20portions%20of%20the%20transcriptome%20oscillated%20in%20phototrophs.%20Periodic%20photosynthesis-related%20transcripts%20exhibited%20a%20temporal%20cascade%20across%20the%20morning%20hours%2C%20conserved%20across%20diverse%20phototrophic%20lineages.%20Pronounced%20silica%3Anitrate%20drawdown%2C%20a%20high%20flavodoxin%20to%20ferredoxin%20transcript%20ratio%2C%20and%20elevated%20expression%20of%20other%20Fe-stress%20markers%20indicated%20Fe-limitation.%20Fe-stress%20markers%20peaked%20during%20a%20photoperiodically%20adaptive%20time%20window%20that%20could%20modulate%20phytoplankton%20response%20to%20seasonal%20Fe-limitation.%20Remarkably%2C%20we%20observed%20viruses%20that%20infect%20the%20majority%20of%20abundant%20taxa%2C%20often%20with%20total%20transcriptional%20activity%20synchronized%20with%20putative%20hosts.%20Taken%20together%2C%20these%20data%20reveal%20a%20microbial%20plankton%20community%20that%20is%20shaped%20by%20recycled%20production%20and%20tightly%20controlled%20by%20Fe-limitation%20and%20viral%20activity.%22%2C%22date%22%3A%222019%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41396-019-0472-2%22%2C%22ISSN%22%3A%221751-7370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2286H9SNJB%22%2C%22WZ6VXZEN%22%5D%2C%22dateModified%22%3A%222022-09-22T23%3A34%3A38Z%22%7D%7D%2C%7B%22key%22%3A%223C8TWCGD%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Schorn%20et%20al.%22%2C%22parsedDate%22%3A%222019-06%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESchorn%2C%20M.%20A.%2C%20Jordan%2C%20P.%20A.%2C%20Podell%2C%20S.%2C%20Blanton%2C%20J.%20M.%2C%20Agarwal%2C%20V.%2C%20Biggs%2C%20J.%20S.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Moore%2C%20B.%20S.%20%282019%29.%20Comparative%20genomics%20of%20cyanobacterial%20symbionts%20reveals%20distinct%2C%20specialized%20metabolism%20in%20tropical%20Dysideidae%20sponges.%20%3Ci%3EMbio%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmBio.00821-19%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmBio.00821-19%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Comparative%20genomics%20of%20cyanobacterial%20symbionts%20reveals%20distinct%2C%20specialized%20metabolism%20in%20tropical%20Dysideidae%20sponges%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michelle%20A.%22%2C%22lastName%22%3A%22Schorn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%20A.%22%2C%22lastName%22%3A%22Jordan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vinayak%22%2C%22lastName%22%3A%22Agarwal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20S.%22%2C%22lastName%22%3A%22Biggs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Margaret%20J.%22%2C%22lastName%22%3A%22McFall-Ngai%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Mohamed%22%2C%22lastName%22%3A%22Donia%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Thacker%22%7D%5D%2C%22abstractNote%22%3A%22Marine%20sponges%20are%20recognized%20as%20valuable%20sources%20of%20bioactive%20metabolites%20and%20renowned%20as%20petri%20dishes%20of%20the%20sea%2C%20providing%20specialized%20niches%20for%20many%20symbiotic%20microorganisms.%20Sponges%20of%20the%20family%20Dysideidae%20are%20well%20documented%20to%20be%20chemically%20talented%2C%20often%20containing%20high%20levels%20of%20polyhalogenated%20compounds%2C%20terpenoids%2C%20peptides%2C%20and%20other%20classes%20of%20bioactive%20small%20molecules.%20This%20group%20of%20tropical%20sponges%20hosts%20a%20high%20abundance%20of%20an%20uncultured%20filamentous%20cyanobacterium%2C%20Hormoscilla%20spongeliae.%20Here%2C%20we%20report%20the%20comparative%20genomic%20analyses%20of%20two%20phylogenetically%20distinct%20Hormoscilla%20populations%2C%20which%20reveal%20shared%20deficiencies%20in%20essential%20pathways%2C%20hinting%20at%20possible%20reasons%20for%20their%20uncultivable%20status%2C%20as%20well%20as%20differing%20biosynthetic%20machinery%20for%20the%20production%20of%20specialized%20metabolites.%20One%20symbiont%20population%20contains%20clustered%20genes%20for%20expanded%20polybrominated%20diphenylether%20%28PBDE%29%20biosynthesis%2C%20while%20the%20other%20instead%20harbors%20a%20unique%20gene%20cluster%20for%20the%20biosynthesis%20of%20the%20dysinosin%20nonribosomal%20peptides.%20The%20hybrid%20sequencing%20and%20assembly%20approach%20utilized%20here%20allows%2C%20for%20the%20first%20time%2C%20a%20comprehensive%20look%20into%20the%20genomes%20of%20these%20elusive%20sponge%20symbionts.IMPORTANCE%20Natural%20products%20provide%20the%20inspiration%20for%20most%20clinical%20drugs.%20With%20the%20rise%20in%20antibiotic%20resistance%2C%20it%20is%20imperative%20to%20discover%20new%20sources%20of%20chemical%20diversity.%20Bacteria%20living%20in%20symbiosis%20with%20marine%20invertebrates%20have%20emerged%20as%20an%20untapped%20source%20of%20natural%20chemistry.%20While%20symbiotic%20bacteria%20are%20often%20recalcitrant%20to%20growth%20in%20the%20lab%2C%20advances%20in%20metagenomic%20sequencing%20and%20assembly%20now%20make%20it%20possible%20to%20access%20their%20genetic%20blueprint.%20A%20cell%20enrichment%20procedure%2C%20combined%20with%20a%20hybrid%20sequencing%20and%20assembly%20approach%2C%20enabled%20detailed%20genomic%20analysis%20of%20uncultivated%20cyanobacterial%20symbiont%20populations%20in%20two%20chemically%20rich%20tropical%20marine%20sponges.%20These%20population%20genomes%20reveal%20a%20wealth%20of%20secondary%20metabolism%20potential%20as%20well%20as%20possible%20reasons%20for%20historical%20difficulties%20in%20their%20cultivation.%22%2C%22date%22%3A%222019%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmBio.00821-19%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222022-08-16T20%3A48%3A34Z%22%7D%7D%2C%7B%22key%22%3A%222ILMVGTZ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Neu%20et%20al.%22%2C%22parsedDate%22%3A%222019-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENeu%2C%20A.%20T.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Roy%2C%20K.%20%282019%29.%20Diversity%20and%20composition%20of%20intertidal%20gastropod%20microbiomes%20across%20a%20major%20marine%20biogeographic%20boundary.%20%3Ci%3EEnvironmental%20Microbiology%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%283%29%2C%20434%26%23x2013%3B447.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1758-2229.12743%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1758-2229.12743%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Diversity%20and%20composition%20of%20intertidal%20gastropod%20microbiomes%20across%20a%20major%20marine%20biogeographic%20boundary%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20T.%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Roy%22%7D%5D%2C%22abstractNote%22%3A%22Marine%20biogeographic%20boundaries%20act%20as%20barriers%20to%20dispersal%20for%20many%20animal%20species%2C%20thereby%20creating%20distinctive%20faunas%20on%20either%20side.%20However%2C%20how%20such%20boundaries%20affect%20the%20distributions%20of%20microbial%20taxa%20remains%20poorly%20known.%20To%20test%20whether%20biogeographic%20boundaries%20influence%20the%20diversity%20and%20composition%20of%20host-associated%20microbiota%2C%20we%20analysed%20the%20microbiomes%20of%20three%20species%20of%20common%20intertidal%20gastropods%20at%20two%20sites%20separated%20by%20the%20biogeographic%20boundary%20at%20Point%20Conception%20%28PtC%29%2C%20CA%2C%20using%2016S%20rRNA%20gene%20sequencing.%20Our%20results%20show%20that%20each%20host%20species%20shows%20microbiome%20compositional%20specificity%2C%20even%20across%20PtC%2C%20and%20that%20alpha%20diversity%20does%20not%20change%20significantly%20across%20this%20boundary%20for%20any%20of%20the%20gastropod%20hosts.%20However%2C%20for%20two%20of%20the%20host%20species%2C%20beta%20diversity%20differs%20significantly%20across%20PtC%2C%20indicating%20that%20there%20may%20be%20multiple%20levels%20of%20organization%20of%20the%20marine%20gastropod%20microbiome.%20Overall%2C%20our%20results%20suggest%20that%20while%20biogeographic%20boundaries%20do%20not%20constrain%20the%20distribution%20of%20a%20core%20set%20of%20microbes%20associated%20with%20each%20host%20species%2C%20they%20can%20play%20a%20role%20in%20structuring%20the%20transient%20portion%20of%20the%20microbiome.%22%2C%22date%22%3A%222019%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2F1758-2229.12743%22%2C%22ISSN%22%3A%221758-2229%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22DBJBKWLL%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Allemann%20et%20al.%22%2C%22parsedDate%22%3A%222019-04%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAllemann%2C%20M.%20N.%2C%20Shulse%2C%20C.%20N.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282019%29.%20Linkage%20of%20marine%20bacteria%20polyunsaturated%20fatty%20acid%20and%20long-chain%20hydrocarbon%20biosynthesis.%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2019.00702%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2019.00702%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Linkage%20of%20marine%20bacteria%20polyunsaturated%20fatty%20acid%20and%20long-chain%20hydrocarbon%20biosynthesis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20N.%22%2C%22lastName%22%3A%22Allemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20N.%22%2C%22lastName%22%3A%22Shulse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Vaious%20marine%20gamma-proteobacteria%20produce%20omega-3%20polyunsaturated%20fatty%20acids%2C%20such%20as%20eicosapentaenoic%20acid%20%2820%3A5%2C%20EPA%29%20and%20docosahexaenoic%20acid%20%2822%3A6%2C%20DHA%29%2C%20which%20are%20incorporated%20into%20membrane%20phospholipids.%20Five%20genes%2C%20designated%20pfaABCDE%2C%20encode%20the%20polyketide%5C%2Ffatty%20acid%20synthase%20necessary%20for%20production%20of%20these%20long-chain%20fatty%20acids.%20In%20addition%20to%20de%20novo%20biosynthesis%20of%20EPA%20and%20DHA%2C%20the%20%5C%22Pfa%20synthase%5C%22%20is%20also%20involved%20with%20production%20of%20a%20long-chain%20polyunsaturated%20hydrocarbon%20product%20%2831%3A9%2C%20PUHC%29%20in%20conjunction%20with%20the%20oleABCD%20hydrocarbon%20biosynthesis%20pathway.%20In%20this%20work%2C%20we%20demonstrate%20that%20OleA%20mediates%20the%20linkage%20between%20these%20two%20pathways%20in%20vivo.%20Co-expression%20of%20pfaA-E%20along%20with%20oleA%20from%20Shewanella%20pealeana%20in%20Escherichia%20coli%20yielded%20the%20expected%20product%2C%20a%2031%3A8%20ketone%20along%20with%20a%20dramatic%20similar%20to%2010-fold%20reduction%20in%20EPA%20content.%20The%20decrease%20in%20EPA%20content%20was%20independent%20of%2031%3A8%20ketone%20production%20as%20co-expression%20of%20an%20OleA%20active%20site%20mutant%20also%20led%20to%20identical%20decreases%20in%20EPA%20content.%20We%20also%20demonstrate%20that%20a%20gene%20linked%20with%20either%20pfa%20and%5C%2For%20ole%20operons%20in%20diverse%20bacterial%20lineages%2C%20herein%20designated%20pfaT%2C%20plays%20a%20role%20in%20maintaining%20optimal%20production%20of%20Pfa%20synthase%20derived%20products%20in%20Photobacterium%20and%20Shewanella%20species.%22%2C%22date%22%3A%222019%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2019.00702%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22WWGS2QJR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Peoples%20et%20al.%22%2C%22parsedDate%22%3A%222019-03%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPeoples%2C%20L.%20M.%2C%20Grammatopoulou%2C%20E.%2C%20Pombrol%2C%20M.%2C%20Xu%2C%20X.%20X.%2C%20Osuntokun%2C%20O.%2C%20Blanton%2C%20J.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Nunnally%2C%20C.%20C.%2C%20Drazen%2C%20J.%20C.%2C%20Mayor%2C%20D.%20J.%2C%20%26amp%3B%20Bartlett%2C%20D.%20H.%20%282019%29.%20Microbial%20community%20diversity%20within%20sediments%20from%20two%20geographically%20separated%20hadal%20trenches.%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2019.00347%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2019.00347%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microbial%20community%20diversity%20within%20sediments%20from%20two%20geographically%20separated%20hadal%20trenches%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20M.%22%2C%22lastName%22%3A%22Peoples%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Grammatopoulou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Pombrol%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22X.%20X.%22%2C%22lastName%22%3A%22Xu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Osuntokun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20C.%22%2C%22lastName%22%3A%22Nunnally%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20C.%22%2C%22lastName%22%3A%22Drazen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20J.%22%2C%22lastName%22%3A%22Mayor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20H.%22%2C%22lastName%22%3A%22Bartlett%22%7D%5D%2C%22abstractNote%22%3A%22Hadal%20ocean%20sediments%2C%20found%20at%20sites%20deeper%20than%206%2C000%20m%20water%20depth%2C%20are%20thought%20to%20contain%20microbial%20communities%20distinct%20from%20those%20at%20shallower%20depths%20due%20to%20high%20hydrostatic%20pressures%20and%20higher%20abundances%20of%20organic%20matter.%20These%20communities%20may%20also%20differ%20from%20one%20other%20as%20a%20result%20of%20geographical%20isolation.%20Here%20we%20compare%20microbial%20community%20composition%20in%20surficial%20sediments%20of%20two%20hadal%20environments-the%20Mariana%20and%20Kermadec%20trenches-to%20evaluate%20microbial%20biogeography%20at%20hadal%20depths.%20Sediment%20microbial%20consortia%20were%20distinct%20between%20trenches%2C%20with%20higher%20relative%20sequence%20abundances%20of%20taxa%20previously%20correlated%20with%20organic%20matter%20degradation%20present%20in%20the%20Kermadec%20Trench.%20In%20contrast%2C%20the%20Mariana%20Trench%2C%20and%20deeper%20sediments%20in%20both%20trenches%2C%20were%20enriched%20in%20taxa%20predicted%20to%20break%20down%20recalcitrant%20material%20and%20contained%20other%20uncharacterized%20lineages.%20At%20the%2097%25%20similarity%20level%2C%20sequence-abundant%20taxa%20were%20not%20trench-specific%20and%20were%20related%20to%20those%20found%20in%20other%20hadal%20and%20abyssal%20habitats%2C%20indicating%20potential%20connectivity%20between%20geographically%20isolated%20sediments.%20Despite%20the%20diversity%20of%20microorganisms%20identified%20using%20culture-independent%20techniques%2C%20most%20isolates%20obtained%20under%20in%20situ%20pressures%20were%20related%20to%20previously%20identified%20piezophiles.%20Members%20related%20to%20these%20same%20taxa%20also%20became%20dominant%20community%20members%20when%20native%20sediments%20were%20incubated%20under%20static%2C%20long-term%2C%20unamended%20high-pressure%20conditions.%20Our%20results%20support%20the%20hypothesis%20that%20there%20is%20connectivity%20between%20sediment%20microbial%20populations%20inhabiting%20the%20Mariana%20and%20Kermadec%20trenches%20while%20showing%20that%20both%20whole%20communities%20and%20specific%20microbial%20lineages%20vary%20between%20trench%20of%20collection%20and%20sediment%20horizon%20depth.%20This%20in%20situ%20biodiversity%20is%20largely%20missed%20when%20incubating%20samples%20within%20pressure%20vessels%20and%20highlights%20the%20need%20for%20revised%20protocols%20for%20high-pressure%20incubations.%22%2C%22date%22%3A%222019%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2019.00347%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22XBFFRTV5%22%5D%2C%22dateModified%22%3A%222022-09-09T21%3A28%3A31Z%22%7D%7D%2C%7B%22key%22%3A%22B3RZD6JM%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222018-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Humphrey%2C%20G.%2C%20Benitez%2C%20R.%20A.%20S.%2C%20Sanders%2C%20J.%2C%20Swofford%2C%20A.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Knight%2C%20R.%20%282018%29.%20High-throughput%20miniaturized%2016S%20rRNA%20amplicon%20library%20preparation%20reduces%20costs%20while%20preserving%20microbiome%20integrity.%20%3Ci%3EMSystems%3C%5C%2Fi%3E%2C%20%3Ci%3E3%3C%5C%2Fi%3E%286%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00166-18%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00166-18%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22High-throughput%20miniaturized%2016S%20rRNA%20amplicon%20library%20preparation%20reduces%20costs%20while%20preserving%20microbiome%20integrity%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G.%22%2C%22lastName%22%3A%22Humphrey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20A.%20S.%22%2C%22lastName%22%3A%22Benitez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Sanders%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Swofford%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Knight%22%7D%5D%2C%22abstractNote%22%3A%22Next%20generation%20sequencing%20technologies%20have%20enabled%20many%20advances%20across%20biology%2C%20with%20microbial%20ecology%20benefiting%20primarily%20through%20expanded%20sample%20sizes.%20Although%20the%20cost%20of%20running%20sequencing%20instruments%20has%20decreased%20substantially%20over%20time%2C%20the%20price%20of%20library%20preparation%20methods%20has%20largely%20remained%20unchanged.%20In%20this%20study%2C%20we%20developed%20a%20low-cost%20miniaturized%20%285-mu%20l%20volume%29%20high-throughput%20%28384-sample%29%20amplicon%20library%20preparation%20method%20with%20the%20Echo%20550%20acoustic%20liquid%20handler.%20Our%20method%20reduces%20costs%20of%20library%20preparation%20to%20%241.42%20per%20sample%2C%20a%2058%25%20reduction%20compared%20to%20existing%20automated%20methods%20and%20a%2021-fold%20reduction%20from%20commercial%20kits%2C%20without%20compromising%20sequencing%20success%20or%20distorting%20the%20microbial%20community%20composition%20analysis.%20We%20further%20validated%20the%20optimized%20method%20by%20sampling%20five%20body%20sites%20from%2046%20Pacific%20chub%20mackerel%20fish%20caught%20across%2016%20sampling%20events%20over%20seven%20months%20from%20the%20Scripps%20Institution%20of%20Oceanography%20pier%20in%20La%20Jolla%2C%20CA.%20Fish%20microbiome%20samples%20were%20processed%20with%20the%20miniaturized%205-mu%20l%20reaction%20volume%20with%200.2%20mu%20l%20of%20genomic%20DNA%20%28gDNA%29%20and%20the%20standard%2025-mu%20l%20reaction%20volume%20with%201%20Al%20of%20gDNA.%20Between%20the%20two%20methods%2C%20alpha%20diversity%20was%20highly%20correlated%20%28R-2%20%3E%200.95%29%2C%20while%20distances%20of%20technical%20replicates%20were%20much%20lower%20than%20with%20inbody-site%20variation%20%28P%20%3C%200.0001%29%2C%20further%20validating%20the%20method.%20The%20cost%20savings%20of%20implementing%20the%20miniaturized%20library%20preparation%20%28going%20from%20triplicate%2025-mu%20l%20reactions%20to%20triplicate%205-mu%20l%20reactions%29%20are%20large%20enough%20to%20cover%20a%20MiSeq%20sequencing%20run%20for%20768%20samples%20while%20preserving%20accurate%20microbiome%20measurements.%20IMPORTANCE%20Reduced%20costs%20of%20sequencing%20have%20tremendously%20impacted%20the%20field%20of%20microbial%20ecology%2C%20allowing%20scientists%20to%20design%20more%20studies%20with%20larger%20sample%20sizes%20that%20often%20exceed%2010%2C000%20samples.%20Library%20preparation%20costs%20have%20not%20kept%20pace%20with%20sequencing%20prices%2C%20although%20automated%20liquid%20handling%20robots%20provide%20a%20unique%20opportunity%20to%20bridge%20this%20gap%20while%20also%20decreasing%20human%20error.%20Here%2C%20we%20take%20advantage%20of%20an%20acoustic%20liquid%20handling%20robot%20to%20develop%20a%20high-throughput%20miniaturized%20library%20preparation%20method%20of%20a%20highly%20cited%20and%20broadly%20used%2016S%20rRNA%20gene%20amplicon%20reaction.%20We%20evaluate%20the%20potential%20negative%20effects%20of%20reducing%20the%20PCR%20volume%20along%20with%20varying%20the%20amount%20of%20gDNA%20going%20into%20the%20reaction.%20Our%20optimized%20method%20reduces%20sample-processing%20costs%20while%20continuing%20to%20generate%20a%20high-quality%20microbiome%20readout%20that%20is%20indistinguishable%20from%20the%20original%20method.%22%2C%22date%22%3A%222018%5C%2F11%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmSystems.00166-18%22%2C%22ISSN%22%3A%222379-5077%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A19Z%22%7D%7D%2C%7B%22key%22%3A%22325YRL37%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Podell%20et%20al.%22%2C%22parsedDate%22%3A%222018-10%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPodell%2C%20S.%2C%20Blanton%2C%20J.%20M.%2C%20Neu%2C%20A.%2C%20Agarwal%2C%20V.%2C%20Biggs%2C%20J.%20S.%2C%20Moore%2C%20B.%20S.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282018%29.%20Pangenomic%20comparison%20of%20globally%20distributed%20Poribacteria%20associated%20with%20sponge%20hosts%20and%20marine%20particles.%20%3Ci%3EThe%20ISME%20Journal%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-018-0292-9%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-018-0292-9%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pangenomic%20comparison%20of%20globally%20distributed%20Poribacteria%20associated%20with%20sponge%20hosts%20and%20marine%20particles%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexander%22%2C%22lastName%22%3A%22Neu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vinayak%22%2C%22lastName%22%3A%22Agarwal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20S.%22%2C%22lastName%22%3A%22Biggs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Candidatus%20Poribacteria%20is%20a%20little-known%20bacterial%20phylum%2C%20previously%20characterized%20by%20partial%20genomes%20from%20a%20single%20sponge%20host%2C%20but%20never%20isolated%20in%20culture.%20We%20have%20reconstructed%20multiple%20genome%20sequences%20from%20four%20different%20sponge%20genera%20and%20compared%20them%20to%20recently%20reported%2C%20uncharacterized%20Poribacteria%20genomes%20from%20the%20open%20ocean%2C%20discovering%20shared%20and%20unique%20functional%20characteristics.%20Two%20distinct%2C%20habitat-linked%20taxonomic%20lineages%20were%20identified%2C%20designated%20Entoporibacteria%20%28sponge-associated%29%20and%20Pelagiporibacteria%20%28free-living%29.%20These%20lineages%20differed%20in%20flagellar%20motility%20and%20chemotaxis%20genes%20unique%20to%20Pelagiporibacteria%2C%20and%20highly%20expanded%20families%20of%20restriction%20endonucleases%2C%20DNA%20methylases%2C%20transposases%2C%20CRISPR%20repeats%2C%20and%20toxin%5Cu2013antitoxin%20gene%20pairs%20in%20Entoporibacteria.%20Both%20lineages%20shared%20pathways%20for%20facultative%20anaerobic%20metabolism%2C%20denitrification%2C%20fermentation%2C%20organosulfur%20compound%20utilization%2C%20type%20IV%20pili%2C%20cellulosomes%2C%20and%20bacterial%20proteosomes.%20Unexpectedly%2C%20many%20features%20characteristic%20of%20eukaryotic%20host%20association%20were%20also%20shared%2C%20including%20genes%20encoding%20the%20synthesis%20of%20eukaryotic-like%20cell%20adhesion%20molecules%2C%20extracellular%20matrix%20digestive%20enzymes%2C%20phosphoinositol-linked%20membrane%20glycolipids%2C%20and%20exopolysaccharide%20capsules.%20Complete%20Poribacteria%2016S%20rRNA%20gene%20sequences%20were%20found%20to%20contain%20multiple%20mismatches%20to%20%5Cu201cuniversal%5Cu201d%2016S%20rRNA%20gene%20primer%20sets%2C%20substantiating%20concerns%20about%20potential%20amplification%20failures%20in%20previous%20studies.%20A%20newly%20designed%20primer%20set%20corrects%20these%20mismatches%2C%20enabling%20more%20accurate%20assessment%20of%20Poribacteria%20abundance%20in%20diverse%20marine%20habitats%20where%20it%20may%20have%20previously%20been%20overlooked.%22%2C%22date%22%3A%222018%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41396-018-0292-9%22%2C%22ISSN%22%3A%221751-7370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222022-09-22T23%3A36%3A19Z%22%7D%7D%2C%7B%22key%22%3A%222G7AKDM4%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222018-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Zhu%2C%20Q.%2C%20Xu%2C%20Z.%20Z.%2C%20Amir%2C%20A.%2C%20Ngochera%2C%20M.%2C%20Simwaka%2C%20M.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Zidana%2C%20H.%2C%20%26amp%3B%20Knight%2C%20R.%20%282018%29.%20Microbial%20effects%20of%20livestock%20manure%20fertilization%20on%20freshwater%20aquaculture%20ponds%20rearing%20tilapia%20%28Oreochromis%20shiranus%29%20and%20North%20African%20catfish%20%28Clarias%20gariepinus%29.%20%3Ci%3EMicrobiologyOpen%3C%5C%2Fi%3E%2C%20%3Ci%3E0%3C%5C%2Fi%3E%280%29%2C%20e00716.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fmbo3.716%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fmbo3.716%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microbial%20effects%20of%20livestock%20manure%20fertilization%20on%20freshwater%20aquaculture%20ponds%20rearing%20tilapia%20%28Oreochromis%20shiranus%29%20and%20North%20African%20catfish%20%28Clarias%20gariepinus%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeremiah%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Qiyun%22%2C%22lastName%22%3A%22Zhu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhenjiang%20Zech%22%2C%22lastName%22%3A%22Xu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amnon%22%2C%22lastName%22%3A%22Amir%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maxon%22%2C%22lastName%22%3A%22Ngochera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Moses%22%2C%22lastName%22%3A%22Simwaka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hastings%22%2C%22lastName%22%3A%22Zidana%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rob%22%2C%22lastName%22%3A%22Knight%22%7D%5D%2C%22abstractNote%22%3A%22The%20majority%20of%20seafood%20is%20farmed%2C%20with%20most%20finfish%20coming%20from%20freshwater%20ponds.%20Ponds%20are%20often%20fertilized%20to%20promote%20microbial%20productivity%20as%20a%20natural%20feed%20source%20to%20fish.%20To%20understand%20if%20pond%20fertilization%20with%20livestock%20manure%20induces%20a%20probiotic%20or%20prebiotic%20effect%2C%20we%20communally%20reared%20tilapia%20%28Oreochromis%20shiranus%29%2C%20and%20North%20African%20catfish%20%28Clarias%20gariepinus%29%2C%20for%204%5Cu00a0weeks%20under%20seven%20manure%20treatments%20including%20layer%20chicken%2C%20broiler%20chicken%2C%20guinea%20fowl%2C%20quail%2C%20pig%2C%20cow%2C%20vs.%20commercial%20feed%20to%20evaluate%20microbial%20community%20dynamics%20of%20the%20manure%2C%20pond%20water%2C%20and%20fish%20feces%20using%2016S%20and%2018S%20rRNA%20marker%20genes%20along%20with%20metagenome%20sequencing.%20Catfish%20growth%2C%20but%20not%20tilapia%2C%20was%20positively%20associated%20with%20plankton%20abundance%20%28p%5Cu00a0%3D%5Cu00a00.0006%2C%20R2%5Cu00a0%3D%5Cu00a00.4887%29%20and%20greatest%20in%20ponds%20fertilized%20with%20quail%20manure%20%28ANOVA%2C%20p%5Cu00a0%3C%5Cu00a00.05%29.%20Manure%20was%20unique%20and%20influenced%20the%2016S%20microbiome%20in%20pond%20water%2C%20tilapia%20gut%2C%20and%20catfish%20gut%20and%2018S%20community%20in%20pond%20water%20and%20catfish%20guts%20%28PERMANOVA%2C%20p%5Cu00a0%3D%5Cu00a00.001%29.%20On%20average%2C%2018.5%25%2C%2018.6%25%2C%20and%2045.3%25%20of%20manure%20bacteria%20sOTUs%2C%20%28sub-operational%20taxonomic%20units%29%2C%20were%20present%20in%20the%20water%20column%2C%20catfish%20feces%2C%20and%20tilapia%20feces%20which%20comprised%203.7%25%2C%2012.8%25%2C%20and%2010.9%25%20of%20the%20total%20microbial%20richness%20of%20the%20communities%2C%20respectively.%20Antibiotic%20resistance%20genes%20were%20highest%20in%20the%20manure%20and%20water%20samples%20followed%20by%20tilapia%20feces%20and%20lowest%20in%20catfish%20feces%20%28p%5Cu00a0%3C%5Cu00a00.0001%29.%20In%20this%20study%2C%20we%20demonstrate%20how%20the%20bacterial%20and%20eukaryotic%20microbial%20composition%20of%20fish%20ponds%20are%20influenced%20by%20specific%20livestock%20manure%20inputs%20and%20that%20the%20gut%20microbiome%20of%20tilapia%20is%20more%20sensitive%20and%20responsive%20than%20catfish%20to%20these%20changes.%20We%20conclude%20that%20animal%20manure%20used%20as%20fertilizer%20induces%20a%20primarily%20prebiotic%20effect%20on%20the%20pond%20ecosystem%20rather%20than%20a%20direct%20probiotic%20effect%20on%20fish.%22%2C%22date%22%3A%222018%5C%2F08%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Fmbo3.716%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22GH74TXJV%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Plominsky%20et%20al.%22%2C%22parsedDate%22%3A%222018-08%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPlominsky%2C%20A.%20M.%2C%20Henriquez-Castillo%2C%20C.%2C%20Delherbe%2C%20N.%2C%20Podell%2C%20S.%2C%20Ramirez-Flandes%2C%20S.%2C%20Ugalde%2C%20J.%20A.%2C%20Santibanez%2C%20J.%20F.%2C%20van%20den%20Engh%2C%20G.%2C%20Hanselmann%2C%20K.%2C%20Ulloa%2C%20O.%2C%20De%20la%20Iglesia%2C%20R.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Trefault%2C%20N.%20%282018%29.%20Distinctive%20archaeal%20composition%20of%20an%20artisanal%20crystallizer%20pond%20and%20functional%20insights%20into%20salt-saturated%20hypersaline%20environment%20adaptation.%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E9%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2018.01800%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2018.01800%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Distinctive%20archaeal%20composition%20of%20an%20artisanal%20crystallizer%20pond%20and%20functional%20insights%20into%20salt-saturated%20hypersaline%20environment%20adaptation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Henriquez-Castillo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Delherbe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Ramirez-Flandes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20A.%22%2C%22lastName%22%3A%22Ugalde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20F.%22%2C%22lastName%22%3A%22Santibanez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G.%22%2C%22lastName%22%3A%22van%20den%20Engh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Hanselmann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Ulloa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22De%20la%20Iglesia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Trefault%22%7D%5D%2C%22abstractNote%22%3A%22Hypersaline%20environments%20represent%20some%20of%20the%20most%20challenging%20settings%20for%20life%20on%20Earth.%20Extremely%20halophilic%20microorganisms%20have%20been%20selected%20to%20colonize%20and%20thrive%20in%20these%20extreme%20environments%20by%20virtue%20of%20a%20broad%20spectrum%20of%20adaptations%20to%20counter%20high%20salinity%20and%20osmotic%20stress.%20Although%20there%20is%20substantial%20data%20on%20microbial%20taxonomic%20diversity%20in%20these%20challenging%20ecosystems%20and%20their%20primary%20osmoadaptation%20mechanisms%2C%20less%20is%20known%20about%20how%20hypersaline%20environments%20shape%20the%20genomes%20of%20microbial%20inhabitants%20at%20the%20functional%20level.%20In%20this%20study%2C%20we%20analyzed%20the%20microbial%20communities%20in%20five%20ponds%20along%20the%20discontinuous%20salinity%20gradient%20from%20brackish%20to%20salt-saturated%20environments%20and%20sequenced%20the%20metagenome%20of%20the%20salt%20%28halite%29%20precipitation%20pond%20in%20the%20artisanal%20Cahuil%20Solar%20Saltern%20system.%20We%20combined%20field%20measurements%20with%20spectrophotometric%20pigment%20analysis%20and%20flow%20cytometry%20to%20characterize%20the%20microbial%20ecology%20of%20the%20pond%20ecosystems%2C%20including%20primary%20producers%20and%20applied%20metagenomic%20sequencing%20for%20analysis%20of%20archaeal%20and%20bacterial%20taxonomic%20diversity%20of%20the%20salt%20crystallizer%20harvest%20pond.%20Comparative%20metagenomic%20analysis%20of%20the%20Cahuil%20salt%20crystallizer%20pond%20against%20microbial%20communities%20from%20other%20salt-saturated%20aquatic%20environments%20revealed%20a%20dominance%20of%20the%20archaeal%20genus%20Halorubrum%20and%20showed%20an%20unexpectedly%20low%20abundance%20of%20Haloquadratum%20in%20the%20Cahuil%20system.%20Functional%20comparison%20of%2026%20hypersaline%20microbial%20metagenomes%20revealed%20a%20high%20proportion%20of%20sequences%20associated%20with%20nucleotide%20excision%20repair%2C%20helicases%2C%20replication%20and%20restriction-methylation%20systems%20in%20all%20of%20them.%20Moreover%2C%20we%20found%20distinctive%20functional%20signatures%20between%20the%20microbial%20communities%20from%20salt-saturated%20%28%3E30%25%20%5Bw%5C%2Fv%5D%20total%20salinity%29%20compared%20to%20sub-saturated%20hypersaline%20environments%20mainly%20due%20to%20a%20higher%20representation%20of%20sequences%20related%20to%20replication%2C%20recombination%20and%20DNA%20repair%20in%20the%20former.%20The%20current%20study%20expands%20our%20understanding%20of%20the%20diversity%20and%20distribution%20of%20halophilic%20microbial%20populations%20inhabiting%20salt-saturated%20habitats%20and%20the%20functional%20attributes%20that%20sustain%20them.%22%2C%22date%22%3A%222018%5C%2F08%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2018.01800%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22GR8NRH4X%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Guillemette%20et%20al.%22%2C%22parsedDate%22%3A%222018-06%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGuillemette%2C%20R.%2C%20Kaneko%2C%20R.%2C%20Blanton%2C%20J.%2C%20Tan%2C%20J.%2C%20Witt%2C%20M.%2C%20Hamilton%2C%20S.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Medina%2C%20M.%2C%20Hamasaki%2C%20K.%2C%20Koch%2C%20B.%20P.%2C%20%26amp%3B%20Azam%2C%20F.%20%282018%29.%20Bacterioplankton%20drawdown%20of%20coral%20mass-spawned%20organic%20matter.%20%3Ci%3EThe%20ISME%20Journal%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-018-0197-7%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-018-0197-7%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Bacterioplankton%20drawdown%20of%20coral%20mass-spawned%20organic%20matter%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ryan%22%2C%22lastName%22%3A%22Guillemette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ryo%22%2C%22lastName%22%3A%22Kaneko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jasmine%22%2C%22lastName%22%3A%22Tan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthias%22%2C%22lastName%22%3A%22Witt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samantha%22%2C%22lastName%22%3A%22Hamilton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00f3nica%22%2C%22lastName%22%3A%22Medina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Koji%22%2C%22lastName%22%3A%22Hamasaki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Boris%20P.%22%2C%22lastName%22%3A%22Koch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Farooq%22%2C%22lastName%22%3A%22Azam%22%7D%5D%2C%22abstractNote%22%3A%22Coral%20reef%20ecosystems%20are%20highly%20sensitive%20to%20microbial%20activities%20that%20result%20from%20dissolved%20organic%20matter%20%28DOM%29%20enrichment%20of%20their%20surrounding%20seawater.%20However%2C%20the%20response%20to%20particulate%20organic%20matter%20%28POM%29%20enrichment%20is%20less%20studied.%20In%20a%20microcosm%20experiment%2C%20we%20tested%20the%20response%20of%20bacterioplankton%20to%20a%20pulse%20of%20POM%20from%20the%20mass-spawning%20of%20Orbicella%20franksi%20coral%20off%20the%20Caribbean%20coast%20of%20Panama.%20Particulate%20organic%20carbon%20%28POC%29%2C%20a%20proxy%20measurement%20for%20POM%2C%20increased%20by%2040-fold%20in%20seawater%20samples%20collected%20during%20spawning%3B%2068%25%20degraded%20within%2066%5Cu2009h.%20The%20elevation%20of%20multiple%20hydrolases%20presumably%20solubilized%20the%20spawn-derived%20POM%20into%20DOM.%20A%20carbon%20budget%20constructed%20for%20the%20275%5Cu2009%5Cu00b5M%20of%20degraded%20POC%20showed%20negligible%20change%20to%20the%20concentration%20of%20dissolved%20organic%20carbon%20%28DOC%29%2C%20indicating%20that%20the%20DOM%20was%20readily%20utilized.%20Fourier%20transform%20ion%20cyclotron%20resonance%20mass%20spectrometry%20shows%20that%20the%20DOM%20pool%20became%20enriched%20with%20heteroatom-containing%20molecules%2C%20a%20trend%20that%20suggests%20microbial%20alteration%20of%20organic%20matter.%20Our%20sensitivity%20analysis%20demonstrates%20that%20bacterial%20carbon%20demand%20could%20have%20accounted%20for%20a%20large%20proportion%20of%20the%20POC%20degradation.%20Further%2C%20using%20bromodeoxyuridine%20immunocapture%20in%20combination%20with%20454%20pyrosequencing%20of%20the%2016S%20ribosomal%20RNA%20gene%2C%20we%20surmise%20that%20actively%20growing%20bacterial%20groups%20were%20the%20primary%20degraders.%20We%20conclude%20that%20coral%20gametes%20are%20highly%20labile%20to%20bacteria%20and%20that%20such%20large%20capacity%20for%20bacterial%20degradation%20and%20alteration%20of%20organic%20matter%20has%20implications%20for%20coral%20reef%20health%20and%20coastal%20marine%20biogeochemistry.%22%2C%22date%22%3A%222018%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41396-018-0197-7%22%2C%22ISSN%22%3A%221751-7370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22ATU2JDYP%22%5D%2C%22dateModified%22%3A%222022-11-21T17%3A55%3A13Z%22%7D%7D%2C%7B%22key%22%3A%22KYCIDECJ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Minich%20et%20al.%22%2C%22parsedDate%22%3A%222018-05%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMinich%2C%20J.%20J.%2C%20Zhu%2C%20Q.%20Y.%2C%20Janssen%2C%20S.%2C%20Hendrickson%2C%20R.%2C%20Amir%2C%20A.%2C%20Vetter%2C%20R.%2C%20Hyde%2C%20J.%2C%20Doty%2C%20M.%20M.%2C%20Stillwell%2C%20K.%2C%20Benardini%2C%20J.%2C%20Kim%2C%20J.%20H.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Venkateswaran%2C%20K.%2C%20%26amp%3B%20Knight%2C%20R.%20%282018%29.%20KatharoSeq%20enables%20high-throughput%20microbiome%20analysis%20from%20low-biomass%20samples.%20%3Ci%3EMSystems%3C%5C%2Fi%3E%2C%20%3Ci%3E3%3C%5C%2Fi%3E%283%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00218-17%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00218-17%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22KatharoSeq%20enables%20high-throughput%20microbiome%20analysis%20from%20low-biomass%20samples%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20J.%22%2C%22lastName%22%3A%22Minich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Q.%20Y.%22%2C%22lastName%22%3A%22Zhu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Janssen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Hendrickson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Amir%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Vetter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Hyde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20M.%22%2C%22lastName%22%3A%22Doty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Stillwell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Benardini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20H.%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Venkateswaran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Knight%22%7D%5D%2C%22abstractNote%22%3A%22Microbiome%20analyses%20of%20low-biomass%20samples%20are%20challenging%20because%20of%20contamination%20and%20inefficiencies%2C%20leading%20many%20investigators%20to%20employ%20low-throughput%20methods%20with%20minimal%20controls.%20We%20developed%20a%20new%20automated%20protocol%2C%20KatharoSeq%20%28from%20the%20Greek%20katharos%20%5Bclean%5D%29%2C%20that%20outperforms%20single-tube%20extractions%20while%20processing%20at%20least%20five%20times%20as%20fast.%20KatharoSeq%20incorporates%20positive%20and%20negative%20controls%20to%20reveal%20the%20whole%20bacterial%20community%20from%20inputs%20of%20as%20few%20as%2050%20cells%20and%20correctly%20identifies%2090.6%25%20%28standard%20error%2C%200.013%25%29%20of%20the%20reads%20from%20500%20cells.%20To%20demonstrate%20the%20broad%20utility%20of%20KatharoSeq%2C%20we%20performed%2016S%20rRNA%20amplicon%20and%20shotgun%20metagenome%20analyses%20of%20the%20Jet%20Propulsion%20Laboratory%20spacecraft%20assembly%20facility%20%28SAF%3B%20n%20%3D%20192%2C%2096%29%2C%2052%20rooms%20of%20a%20neonatal%20intensive%20care%20unit%20%28NICU%3B%20n%20%3D%20388%2C%20337%29%2C%20and%20an%20endangered-abalone-rearing%20facility%20%28n%20%3D%20192%2C%20123%29%2C%20obtaining%20spatially%20resolved%2C%20unique%20microbiomes%20reproducible%20across%20hundreds%20of%20samples.%20The%20SAF%2C%20our%20primary%20focus%2C%20contains%2032%20sOTUs%20%28sub-OTUs%2C%20defined%20as%20exact%20sequence%20matches%29%20and%20their%20inferred%20variants%20identified%20by%20the%20deblur%20algorithm%2C%20with%20four%20%28Acinetobacter%20lwoffii%2C%20Paracoccus%20marcusii%2C%20Mycobacterium%20sp.%2C%20and%20Novosphingobium%29%20being%20present%20in%20%3E75%25%20of%20the%20samples.%20According%20to%20microbial%20spatial%20topography%2C%20the%20most%20abundant%20cleanroom%20contaminant%2C%20A.%20lwoffii%2C%20is%20related%20to%20human%20foot%20traffic%20exposure.%20In%20the%20NICU%2C%20we%20have%20been%20able%20to%20discriminate%20environmental%20exposure%20related%20to%20patient%20infectious%20disease%2C%20and%20in%20the%20abalone%20facility%2C%20we%20show%20that%20microbial%20communities%20reflect%20the%20marine%20environment%20rather%20than%20human%20input.%20Consequently%2C%20we%20demonstrate%20the%20feasibility%20and%20utility%20of%20large-scale%2C%20low-biomass%20metagenomic%20analyses%20using%20the%20KatharoSeq%20protocol.%20IMPORTANCE%20Various%20indoor%2C%20outdoor%2C%20and%20host-associated%20environments%20contain%20small%20quantities%20of%20microbial%20biomass%20and%20represent%20a%20niche%20that%20is%20often%20understudied%20because%20of%20technical%20constraints.%20Many%20studies%20that%20attempt%20to%20evaluate%20these%20low-biomass%20microbiome%20samples%20are%20riddled%20with%20erroneous%20results%20that%20are%20typically%20false%20positive%20signals%20obtained%20during%20the%20sampling%20process.%20We%20have%20investigated%20various%20low-biomass%20kits%20and%20methods%20to%20determine%20the%20limit%20of%20detection%20of%20these%20pipelines.%20Here%20we%20present%20KatharoSeq%2C%20a%20high-throughput%20protocol%20combining%20laboratory%20and%20bioinformatic%20methods%20that%20can%20differentiate%20a%20true%20positive%20signal%20in%20samples%20with%20as%20few%20as%2050%20to%20500%20cells.%20We%20demonstrate%20the%20application%20of%20this%20method%20in%20three%20unique%20low-biomass%20environments%2C%20including%20a%20SAF%2C%20a%20hospital%20NICU%2C%20and%20an%20abalone-rearing%20facility.%22%2C%22date%22%3A%222018%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmSystems.00218-17%22%2C%22ISSN%22%3A%222379-5077%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A19Z%22%7D%7D%2C%7B%22key%22%3A%22R4H7JALE%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Peoples%20et%20al.%22%2C%22parsedDate%22%3A%222018-04%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPeoples%2C%20L.%20M.%2C%20Donaldson%2C%20S.%2C%20Osuntokun%2C%20O.%2C%20Xia%2C%20Q.%2C%20Nelson%2C%20A.%2C%20Blanton%2C%20J.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Church%2C%20M.%20J.%2C%20%26amp%3B%20Bartlett%2C%20D.%20H.%20%282018%29.%20Vertically%20distinct%20microbial%20communities%20in%20the%20Mariana%20and%20Kermadec%20trenches.%20%3Ci%3EPLOS%20ONE%3C%5C%2Fi%3E%2C%20%3Ci%3E13%3C%5C%2Fi%3E%284%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0195102%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0195102%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Vertically%20distinct%20microbial%20communities%20in%20the%20Mariana%20and%20Kermadec%20trenches%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20M.%22%2C%22lastName%22%3A%22Peoples%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Donaldson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Osuntokun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Q.%22%2C%22lastName%22%3A%22Xia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Nelson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20J.%22%2C%22lastName%22%3A%22Church%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20H.%22%2C%22lastName%22%3A%22Bartlett%22%7D%5D%2C%22abstractNote%22%3A%22Hadal%20trenches%2C%20oceanic%20locations%20deeper%20than%206%2C000%20m%2C%20are%20thought%20to%20have%20distinct%20microbial%20communities%20compared%20to%20those%20at%20shallower%20depths%20due%20to%20high%20hydrostatic%20pressures%2C%20topographical%20funneling%20of%20organic%20matter%2C%20and%20biogeographical%20isolation.%20Here%20we%20evaluate%20the%20hypothesis%20that%20hadal%20trenches%20contain%20unique%20microbial%20biodiversity%20through%20analyses%20of%20the%20communities%20present%20in%20the%20bottom%20waters%20of%20the%20Kermadec%20and%20Mariana%20trenches.%20Estimates%20of%20microbial%20protein%20production%20indicate%20active%20populations%20under%20in%20situ%20hydrostatic%20pressures%20and%20increasing%20adaptation%20to%20pressure%20with%20depth.%20Depth%2C%20trench%20of%20collection%2C%20and%20size%20fraction%20are%20important%20drivers%20of%20microbial%20community%20structure.%20Many%20putative%20hadal%20bathytypes%2C%20such%20as%20members%20related%20to%20the%20Marinimicrobia%2C%20Rhodobacteraceae%2C%20Rhodospirilliceae%2C%20and%20Aquibacter%2C%20are%20similar%20to%20members%20identified%20in%20other%20trenches.%20Most%20of%20the%20differences%20between%20the%20two%20trench%20microbiomes%20consists%20of%20taxa%20belonging%20to%20the%20Gammaproteobacteria%20whose%20distributions%20extend%20throughout%20the%20water%20column.%20Growth%20and%20survival%20estimates%20of%20representative%20isolates%20of%20these%20taxa%20under%20deep-sea%20conditions%20suggest%20that%20some%20members%20may%20descend%20from%20shallower%20depths%20and%20exist%20as%20a%20potentially%20inactive%20fraction%20of%20the%20hadal%20zone.%20We%20conclude%20that%20the%20distinct%20pelagic%20communities%20residing%20in%20these%20two%20trenches%2C%20and%20perhaps%20by%20extension%20other%20trenches%2C%20reflect%20both%20cosmopolitan%20hadal%20bathytypes%20and%20ubiquitous%20genera%20found%20throughout%20the%20water%20column.%22%2C%22date%22%3A%222018%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0195102%22%2C%22ISSN%22%3A%221932-6203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22XBFFRTV5%22%5D%2C%22dateModified%22%3A%222022-11-21T21%3A35%3A24Z%22%7D%7D%2C%7B%22key%22%3A%222VCVNTAA%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Plominsky%20et%20al.%22%2C%22parsedDate%22%3A%222018-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPlominsky%2C%20A.%20M.%2C%20Trefault%2C%20N.%2C%20Podell%2C%20S.%2C%20Blanton%2C%20J.%20M.%2C%20De%20la%20Iglesia%2C%20R.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20von%20Dassow%2C%20P.%2C%20%26amp%3B%20Ulloa%2C%20O.%20%282018%29.%20Metabolic%20potential%20and%20in%20situ%20transcriptomic%20profiles%20of%20previously%20uncharacterized%20key%20microbial%20groups%20involved%20in%20coupled%20carbon%2C%20nitrogen%20and%20sulfur%20cycling%20in%20anoxic%20marine%20zones.%20%3Ci%3EEnvironmental%20Microbiology%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1462-2920.14109%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1462-2920.14109%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Metabolic%20potential%20and%20in%20situ%20transcriptomic%20profiles%20of%20previously%20uncharacterized%20key%20microbial%20groups%20involved%20in%20coupled%20carbon%2C%20nitrogen%20and%20sulfur%20cycling%20in%20anoxic%20marine%20zones%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alvaro%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicole%22%2C%22lastName%22%3A%22Trefault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodrigo%22%2C%22lastName%22%3A%22De%20la%20Iglesia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22von%20Dassow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Osvaldo%22%2C%22lastName%22%3A%22Ulloa%22%7D%5D%2C%22abstractNote%22%3A%22Summary%20Anoxic%20marine%20zones%20%28AMZs%29%20impact%20biogeochemical%20cycles%20at%20the%20global%20scale%2C%20particularly%20the%20nitrogen%20cycle.%20Key%20microbial%20players%20from%20AMZs%20have%20been%20identified%2C%20but%20the%20majority%20remains%20unrecognized%20or%20uncharacterized.%20Thirty-one%20single-cell%20amplified%20genomes%20%28SAGs%29%20from%20the%20eastern%20tropical%20North%20and%20South%20Pacific%20AMZs%20were%20sequenced%20to%20gain%20insight%20into%20the%20distribution%2C%20metabolic%20potential%20and%20contribution%20to%20the%20community%20transcriptional%20profile%20of%20these%20uncharacterized%20bacterial%20and%20archaeal%20groups.%20Detailed%20analyses%20focused%20on%20SAG-bins%20assigned%20to%20three%20of%20these%20groups%20that%20presented%2079%25%3F100%25%20estimated%20genome%20completeness%3A%20the%20putative%20sulphur-oxidizing%20Gamaproteobacteria%20EOSA%20II%20clade%2C%20a%20Marinimicrobia%20member%20of%20the%20recently%20recognized%20PN262000N21%20clade%20found%20to%20be%20abundant%20in%20AMZ%20anoxic%20cores%2C%20and%20a%20representative%20of%20the%20Marine%20Benthic%20Group%20A%20Thaumarchaeota.%20Community-based%20analyses%20revealed%20that%20these%20three%20groups%20are%20significantly%20more%20abundant%20and%20transcriptionally%20more%20active%20in%20the%20AMZ%20microbial%20communities%20than%20previously%20described%20phylogenetically%20related%20microbial%20groups.%20Collectively%2C%20these%20groups%20have%20the%20potential%20to%20link%20biogeochemically%20relevant%20processes%20by%20coupling%20the%20carbon%2C%20nitrogen%20and%20sulfur%20cycles.%20Together%2C%20these%20results%20increase%20our%20understanding%20of%20key%20microbial%20components%20inhabiting%20AMZs%20and%20other%20oxygen-deficient%20marine%20environments%2C%20enhancing%20our%20capacity%20to%20predict%20the%20impact%20of%20the%20expansion%20of%20these%20ecosystems%20due%20to%20climate%20change.%22%2C%22date%22%3A%222018%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2F1462-2920.14109%22%2C%22ISSN%22%3A%221462-2912%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22YZUQ2LXM%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Allemann%20and%20Allen%22%2C%22parsedDate%22%3A%222018-01%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAllemann%2C%20M.%20N.%2C%20%26amp%3B%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%20%282018%29.%20Characterization%20and%20Application%20of%20Marine%20Microbial%20Omega-3%20Polyunsaturated%20Fatty%20Acid%20Synthesis.%20In%20B.%20S.%20Moore%20%28Ed.%29%2C%20%3Ci%3EMethods%20in%20Enzymology%3C%5C%2Fi%3E%20%28Vol.%20605%2C%20pp.%203%26%23x2013%3B32%29.%20Academic%20Press.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22bookSection%22%2C%22title%22%3A%22Characterization%20and%20Application%20of%20Marine%20Microbial%20Omega-3%20Polyunsaturated%20Fatty%20Acid%20Synthesis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marco%20N.%22%2C%22lastName%22%3A%22Allemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%5D%2C%22abstractNote%22%3A%22The%20long-chain%20omega-3%20polyunsaturated%20fatty%20acids%20%28n-3%20LC-PUFAs%29%20EPA%20%2820%3A5n-3%29%20and%20DHA%20%2822%3A6n-3%29%20are%20widely%20recognized%20as%20beneficial%20to%20human%20health%20and%20development.%20Select%20lineages%20of%20cosmopolitan%20marine%20prokaryotic%20and%20eukaryotic%20microorganisms%20synthesize%20these%20compounds%20via%20a%20unique%20fatty%20acid%20synthase%5C%2Fpolyketide%20synthase%20mechanism%20that%20is%20distinct%20from%20the%20canonical%20desaturase%5C%2Felongase-mediated%20pathway%20employed%20by%20the%20majority%20of%20eukaryotic%20single-cell%20microorganisms%20and%20metazoans.%20This%20%5Cu201cPfa%20synthase%5Cu201d%20mechanism%20is%20highly%20efficient%20and%20has%20been%20co-opted%20for%20the%20large-scale%20industrial%20production%20of%20n-3%20LC-PUFAs%20for%20commercial%20applications.%20Both%20prokaryotic%20and%20eukaryotic%20microbes%20containing%20this%20pathway%20can%20be%20readily%20isolated%20from%20marine%20environments%20and%20maintained%20in%20culture%20under%20laboratory%20conditions.%20Some%20strains%20are%20genetically%20tractable%20and%20have%20established%20methods%20for%20genetic%20modification.%20The%20discussion%20and%20methods%20presented%20here%20should%20be%20useful%20for%20the%20exploitation%20and%20optimization%20of%20n-3%20LC-PUFA%20products%20from%20marine%20microorganisms.%22%2C%22bookTitle%22%3A%22Methods%20in%20Enzymology%22%2C%22date%22%3A%222018%5C%2F01%22%2C%22language%22%3A%22%22%2C%22ISBN%22%3A%220076-6879%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22NSJWUN43%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Leao%20et%20al.%22%2C%22parsedDate%22%3A%222017-03%22%2C%22numChildren%22%3A8%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELeao%2C%20T.%2C%20Castelao%2C%20G.%2C%20Korobeynikov%2C%20A.%2C%20Monroe%2C%20E.%20A.%2C%20Podell%2C%20S.%2C%20Glukhov%2C%20E.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Gerwick%2C%20W.%20H.%2C%20%26amp%3B%20Gerwick%2C%20L.%20%282017%29.%20Comparative%20genomics%20uncovers%20the%20prolific%20and%20distinctive%20metabolic%20potential%20of%20the%20cyanobacterial%20genus%20Moorea.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E114%3C%5C%2Fi%3E%2812%29%2C%203198%26%23x2013%3B3203.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1618556114%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1618556114%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Comparative%20genomics%20uncovers%20the%20prolific%20and%20distinctive%20metabolic%20potential%20of%20the%20cyanobacterial%20genus%20Moorea%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Leao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G.%22%2C%22lastName%22%3A%22Castelao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Korobeynikov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20A.%22%2C%22lastName%22%3A%22Monroe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Glukhov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22W.%20H.%22%2C%22lastName%22%3A%22Gerwick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Gerwick%22%7D%5D%2C%22abstractNote%22%3A%22Cyanobacteria%20are%20major%20sources%20of%20oxygen%2C%20nitrogen%2C%20and%20carbon%20in%20nature.%20In%20addition%20to%20the%20importance%20of%20their%20primary%20metabolism%2C%20some%20cyanobacteria%20are%20prolific%20producers%20of%20unique%20and%20bioactive%20secondary%20metabolites.%20Chemical%20investigations%20of%20the%20cyanobacterial%20genus%20Moorea%20have%20resulted%20in%20the%20isolation%20of%20over%20190%20compounds%20in%20the%20last%20two%20decades.%20However%2C%20preliminary%20genomic%20analysis%20has%20suggested%20that%20genome-guided%20approaches%20can%20enable%20the%20discovery%20of%20novel%20compounds%20from%20even%20well-studied%20Moorea%20strains%2C%20highlighting%20the%20importance%20of%20obtaining%20complete%20genomes.%20We%20report%20a%20complete%20genome%20of%20a%20filamentous%20tropical%20marine%20cyanobacterium%2C%20Moorea%20producens%20PAL%2C%20which%20reveals%20that%20about%20one-fifth%20of%20its%20genome%20is%20devoted%20to%20production%20of%20secondary%20metabolites%2C%20an%20impressive%20four%20times%20the%20cyanobacterial%20average.%20Moreover%2C%20possession%20of%20the%20complete%20PAL%20genome%20has%20allowed%20improvement%20to%20the%20assembly%20of%20three%20other%20Moorea%20draft%20genomes.%20Comparative%20genomics%20revealed%20that%20they%20are%20remarkably%20similar%20to%20one%20another%2C%20despite%20their%20differences%20in%20geography%2C%20morphology%2C%20and%20secondary%20metabolite%20profiles.%20Gene%20cluster%20networking%20highlights%20that%20this%20genus%20is%20distinctive%20among%20cyanobacteria%2C%20not%20only%20in%20the%20number%20of%20secondary%20metabolite%20pathways%20but%20also%20in%20the%20content%20of%20many%20pathways%2C%20which%20are%20potentially%20distinct%20from%20all%20other%20bacterial%20gene%20clusters%20to%20date.%20These%20findings%20portend%20that%20future%20genome-guided%20secondary%20metabolite%20discovery%20and%20isolation%20efforts%20should%20be%20highly%20productive.%22%2C%22date%22%3A%222017%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1618556114%22%2C%22ISSN%22%3A%220027-8424%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%222N8J54Y9%22%2C%229BHAPH7Z%22%2C%22X8P588WG%22%5D%2C%22dateModified%22%3A%222023-02-17T18%3A25%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22SKTV2TRB%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Agarwal%20et%20al.%22%2C%22parsedDate%22%3A%222017-03%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAgarwal%2C%20V.%2C%20Blanton%2C%20J.%20M.%2C%20Podell%2C%20S.%2C%20Taton%2C%20A.%2C%20Schorn%2C%20M.%20A.%2C%20Busch%2C%20J.%2C%20Lin%2C%20Z.%2C%20Schmidt%2C%20E.%20W.%2C%20Jensen%2C%20P.%20R.%2C%20Paul%2C%20V.%20J.%2C%20Biggs%2C%20J.%20S.%2C%20Golden%2C%20J.%20W.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Moore%2C%20B.%20S.%20%282017%29.%20Metagenomic%20discovery%20of%20polybrominated%20diphenyl%20ether%20biosynthesis%20by%20marine%20sponges.%20%3Ci%3ENat%20Chem%20Biol%3C%5C%2Fi%3E%2C%20%3Ci%3Eadvance%20online%20publication%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnchembio.2330%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnchembio.2330%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Metagenomic%20discovery%20of%20polybrominated%20diphenyl%20ether%20biosynthesis%20by%20marine%20sponges%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vinayak%22%2C%22lastName%22%3A%22Agarwal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arnaud%22%2C%22lastName%22%3A%22Taton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michelle%20A.%22%2C%22lastName%22%3A%22Schorn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Busch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhenjian%22%2C%22lastName%22%3A%22Lin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20W.%22%2C%22lastName%22%3A%22Schmidt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%20R.%22%2C%22lastName%22%3A%22Jensen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valerie%20J.%22%2C%22lastName%22%3A%22Paul%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20S.%22%2C%22lastName%22%3A%22Biggs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%20W.%22%2C%22lastName%22%3A%22Golden%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bradley%20S.%22%2C%22lastName%22%3A%22Moore%22%7D%5D%2C%22abstractNote%22%3A%22Naturally%20produced%20polybrominated%20diphenyl%20ethers%20%28PBDEs%29%20pervade%20the%20marine%20environment%20and%20structurally%20resemble%20toxic%20man-made%20brominated%20flame%20retardants.%20PBDEs%20bioaccumulate%20in%20marine%20animals%20and%20are%20likely%20transferred%20to%20the%20human%20food%20chain.%20However%2C%20the%20biogenic%20basis%20for%20PBDE%20production%20in%20one%20of%20their%20most%20prolific%20sources%2C%20marine%20sponges%20of%20the%20order%20Dysideidae%2C%20remains%20unidentified.%20Here%2C%20we%20report%20the%20discovery%20of%20PBDE%20biosynthetic%20gene%20clusters%20within%20sponge-microbiome-associated%20cyanobacterial%20endosymbionts%20through%20the%20use%20of%20an%20unbiased%20metagenome-mining%20approach.%20Using%20expression%20of%20PBDE%20biosynthetic%20genes%20in%20heterologous%20cyanobacterial%20hosts%2C%20we%20correlate%20the%20structural%20diversity%20of%20naturally%20produced%20PBDEs%20to%20modifications%20within%20PBDE%20biosynthetic%20gene%20clusters%20in%20multiple%20sponge%20holobionts.%20Our%20results%20establish%20the%20genetic%20and%20molecular%20foundation%20for%20the%20production%20of%20PBDEs%20in%20one%20of%20the%20most%20abundant%20natural%20sources%20of%20these%20molecules%2C%20further%20setting%20the%20stage%20for%20a%20metagenomic-based%20inventory%20of%20other%20PBDE%20sources%20in%20the%20marine%20environment.%22%2C%22date%22%3A%222017%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fnchembio.2330%22%2C%22ISSN%22%3A%221552-4469%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22G2HWKXNT%22%2C%22R4QPIR6K%22%2C%22IWNPIQDK%22%5D%2C%22dateModified%22%3A%222022-08-15T17%3A04%3A46Z%22%7D%7D%2C%7B%22key%22%3A%22HJ5F37JA%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Demidenko%20et%20al.%22%2C%22parsedDate%22%3A%222017-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDemidenko%2C%20A.%2C%20Akberdinl%2C%20I.%20R.%2C%20Allemann%2C%20M.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Kalyuzhnaya%2C%20M.%20G.%20%282017%29.%20Fatty%20acid%20biosynthesis%20pathways%20in%20Methylomicrobium%20buryatense%205G%28B1%29.%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E7%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2016.02167%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2016.02167%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Fatty%20acid%20biosynthesis%20pathways%20in%20Methylomicrobium%20buryatense%205G%28B1%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Demidenko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20R.%22%2C%22lastName%22%3A%22Akberdinl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Allemann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20G.%22%2C%22lastName%22%3A%22Kalyuzhnaya%22%7D%5D%2C%22abstractNote%22%3A%22Methane%20utilization%20by%20methanotrophic%20bacteria%20is%20an%20attractive%20application%20for%20biotechnological%20conversion%20of%20natural%20or%20biogas%20into%20high-added-value%20products.%20Haloalcaliphilic%20methanotrophic%20bacteria%20belonging%20to%20the%20genus%20Methylomicrobium%20are%20among%20the%20most%20promising%20strains%20for%20methane%20based%20biotechnology%2C%20providing%20easy%20and%20inexpensive%20cultivation%2C%20rapid%20growth%2C%20and%20the%20availability%20of%20established%20genetic%20tools.%20A%20number%20of%20methane%20bioconversions%20using%20these%20microbial%20cultures%20have%20been%20discussed%2C%20including%20the%20derivation%20of%20biodiesel%2C%20alkanes%2C%20and%20OMEGA-3%20supplements.%20These%20compounds%20are%20derived%20from%20bacterial%20fatty%20acid%20pools.%20Here%2C%20we%20investigate%20fatty%20acid%20biosynthesis%20in%20Methylomicrobium%20buryatense%205G%28B1%29.%20Most%20of%20the%20genes%20homologous%20to%20typical%20Type%20II%20fatty%20acid%20biosynthesis%20pathways%20could%20be%20annotated%20by%20bioinformatics%20analyses%2C%20with%20the%20exception%20of%20fatty%20acid%20transport%20and%20regulatory%20elements.%20Different%20approaches%20for%20improving%20fatty%20acid%20accumulation%20were%20investigated.%20These%20studies%20indicated%20that%20both%20fatty%20acid%20degradation%20and%20acetyl-%20and%20malonyl-CoA%20levels%20are%20bottlenecks%20for%20higher%20level%20fatty%20acid%20production.%20The%20best%20strain%20generated%20in%20this%20study%20synthesizes%20111%20%2B%5C%2F-%202%20mg%5C%2FgDCW%20of%20extractable%20fatty%20acids%2C%20which%20is%2020%25%20more%20than%20the%20original%20strain.%20A%20candidate%20gene%20for%20fatty%20acid%20biosynthesis%20regulation%2C%20farE%2C%20was%20identified%20and%20studied.%20Its%20deletion%20resulted%20in%20drastic%20changes%20to%20the%20fatty%20acid%20profile%2C%20leading%20to%20an%20increased%20pool%20of%20C18-fatty%20acid%20methyl%20ester.%20The%20FarE-regulon%20was%20further%20investigated%20by%20RNA-seq%20analysis%20of%20gene%20expression%20in%20farE-knockout%20mutants%20and%20farE-overexpressing%20strains.%20These%20gene%20profiles%20highlighted%20a%20novel%20set%20of%20enzymes%20and%20regulators%20involved%20in%20fatty%20acid%20biosynthesis.%20The%20gene%20expression%20and%20fatty%20acid%20profiles%20of%20the%20different%20farE-strains%20support%20the%20hypothesis%20that%20metabolic%20fluxes%20upstream%20of%20fatty%20acid%20biosynthesis%20restrict%20fatty%20acid%20production%20in%20the%20methanotroph.%22%2C%22date%22%3A%222017%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2016.02167%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22FR54GX58%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Paerl%20et%20al.%22%2C%22parsedDate%22%3A%222016-12%22%2C%22numChildren%22%3A6%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPaerl%2C%20R.%20W.%2C%20Bouget%2C%20F.-Y.%2C%20Lozano%2C%20J.-C.%2C%20Verge%2C%20V.%2C%20Schatt%2C%20P.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Palenik%2C%20B.%2C%20%26amp%3B%20Azam%2C%20F.%20%282016%29.%20Use%20of%20plankton-derived%20vitamin%20B1%20precursors%2C%20especially%20thiazole-related%20precursor%2C%20by%20key%20marine%20picoeukaryotic%20phytoplankton.%20%3Ci%3EIsme%20Journal%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fismej.2016.145%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fismej.2016.145%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Use%20of%20plankton-derived%20vitamin%20B1%20precursors%2C%20especially%20thiazole-related%20precursor%2C%20by%20key%20marine%20picoeukaryotic%20phytoplankton%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ryan%20W.%22%2C%22lastName%22%3A%22Paerl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Yves%22%2C%22lastName%22%3A%22Bouget%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Claude%22%2C%22lastName%22%3A%22Lozano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valerie%22%2C%22lastName%22%3A%22Verge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Schatt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brian%22%2C%22lastName%22%3A%22Palenik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Farooq%22%2C%22lastName%22%3A%22Azam%22%7D%5D%2C%22abstractNote%22%3A%22Several%20cosmopolitan%20marine%20picoeukaryotic%20phytoplankton%20are%20B1%20auxotrophs%20requiring%20exogenous%20vitamin%20B1%20or%20precursor%20to%20survive.%20From%20genomic%20evidence%2C%20representatives%20of%20picoeukaryotic%20phytoplankton%20%28Ostreococcus%20and%20Micromonas%20spp.%29%20were%20predicted%20to%20use%20known%20thiazole%20and%20pyrimidine%20B1%20precursors%20to%20meet%20their%20B1%20demands%2C%20however%2C%20recent%20culture-based%20experiments%20could%20not%20confirm%20this%20assumption.%20We%20hypothesized%20these%20phytoplankton%20strains%20could%20grow%20on%20precursors%20alone%2C%20but%20required%20a%20thiazole-related%20precursor%20other%20the%20well-known%20and%20extensively%20tested%204-methyl-5-thiazoleethanol.%20This%20hypothesis%20was%20tested%20using%20bioassays%20and%20co-cultures%20of%20picoeukaryotic%20phytoplankton%20and%20bacteria.%20We%20found%20that%20specific%20B1-synthesizing%20proteobacteria%20and%20phytoplankton%20are%20sources%20of%20a%20yet-to-be%20chemically%20identified%20thiazole-related%20precursor%28s%29%20that%2C%20along%20with%20pyrimidine%20B1%20precursor%204-amino-5-hydroxymethyl-2-methylpyrimidine%2C%20can%20support%20growth%20of%20Ostreococcus%20spp.%20%28also%20Micromonas%20spp.%29%20without%20B1.%20We%20additionally%20found%20that%20the%20B1-synthesizing%20plankton%20do%20not%20require%20contact%20with%20picoeukaryotic%20phytoplankton%20cells%20to%20produce%20thiazole-related%20precursor%28s%29.%20Experiments%20with%20wild-type%20and%20genetically%20engineered%20Ostreococcus%20lines%20revealed%20that%20the%20thiazole%20kinase%2C%20ThiM%2C%20is%20required%20for%20growth%20on%20precursors%2C%20and%20that%20thiazole-related%20precursor%28s%29%20accumulate%20to%20appreciable%20levels%20in%20the%20euphotic%20ocean.%20Overall%2C%20our%20results%20point%20to%20thiazole-related%20B1%20precursors%20as%20important%20micronutrients%20promoting%20the%20survival%20of%20abundant%20phytoplankton%20influencing%20surface%20ocean%20production%20and%20biogeochemical%20cycling.%22%2C%22date%22%3A%222016%5C%2F12%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fismej.2016.145%22%2C%22ISSN%22%3A%221751-7370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22ATU2JDYP%22%2C%22BSCQ9CW7%22%5D%2C%22dateModified%22%3A%222023-04-10T22%3A53%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22AA88Q3HA%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Traller%20et%20al.%22%2C%22parsedDate%22%3A%222016-11%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETraller%2C%20J.%20C.%2C%20Cokus%2C%20S.%20J.%2C%20Lopez%2C%20D.%20A.%2C%20Gaidarenko%2C%20O.%2C%20Smith%2C%20S.%20R.%2C%20McCrow%2C%20J.%20P.%2C%20Gallaher%2C%20S.%20D.%2C%20Podell%2C%20S.%2C%20Thompson%2C%20M.%2C%20Cook%2C%20O.%2C%20Morselli%2C%20M.%2C%20Jaroszewicz%2C%20A.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Allen%2C%20A.%20E.%2C%20Merchant%2C%20S.%20S.%2C%20Pellegrini%2C%20M.%2C%20%26amp%3B%20Hildebrand%2C%20M.%20%282016%29.%20Genome%20and%20methylome%20of%20the%20oleaginous%20diatom%20Cyclotella%20cryptica%20reveal%20genetic%20flexibility%20toward%20a%20high%20lipid%20phenotype.%20%3Ci%3EBiotechnology%20for%20Biofuels%3C%5C%2Fi%3E%2C%20%3Ci%3E9%3C%5C%2Fi%3E%281%29%2C%20258.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13068-016-0670-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13068-016-0670-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Genome%20and%20methylome%20of%20the%20oleaginous%20diatom%20Cyclotella%20cryptica%20reveal%20genetic%20flexibility%20toward%20a%20high%20lipid%20phenotype%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jesse%20C.%22%2C%22lastName%22%3A%22Traller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shawn%20J.%22%2C%22lastName%22%3A%22Cokus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20A.%22%2C%22lastName%22%3A%22Lopez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%22%2C%22lastName%22%3A%22Gaidarenko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarah%20R.%22%2C%22lastName%22%3A%22Smith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20P.%22%2C%22lastName%22%3A%22McCrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sean%20D.%22%2C%22lastName%22%3A%22Gallaher%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheila%22%2C%22lastName%22%3A%22Podell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Thompson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Orna%22%2C%22lastName%22%3A%22Cook%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marco%22%2C%22lastName%22%3A%22Morselli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Artur%22%2C%22lastName%22%3A%22Jaroszewicz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabeeha%20S.%22%2C%22lastName%22%3A%22Merchant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matteo%22%2C%22lastName%22%3A%22Pellegrini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mark%22%2C%22lastName%22%3A%22Hildebrand%22%7D%5D%2C%22abstractNote%22%3A%22Improvement%20in%20the%20performance%20of%20eukaryotic%20microalgae%20for%20biofuel%20and%20bioproduct%20production%20is%20largely%20dependent%20on%20characterization%20of%20metabolic%20mechanisms%20within%20the%20cell.%20The%20marine%20diatom%20Cyclotella%20cryptica%2C%20which%20was%20originally%20identified%20in%20the%20Aquatic%20Species%20Program%2C%20is%20a%20promising%20strain%20of%20microalgae%20for%20large-scale%20production%20of%20biofuel%20and%20bioproducts%2C%20such%20as%20omega-3%20fatty%20acids.%22%2C%22date%22%3A%222016%5C%2F11%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs13068-016-0670-3%22%2C%22ISSN%22%3A%221754-6834%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%2286H9SNJB%22%2C%22N2G3EE7H%22%5D%2C%22dateModified%22%3A%222023-04-10T22%3A53%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22MVLRICXI%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222016-10%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20Bundy%2C%20R.%20M.%2C%20Blanton%2C%20J.%20M.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20%26amp%3B%20Barbeau%2C%20K.%20A.%20%282016%29.%20Copiotrophic%20marine%20bacteria%20are%20associated%20with%20strong%20iron-binding%20ligand%20production%20during%20phytoplankton%20blooms.%20%3Ci%3ELimnology%20and%20Oceanography%20Letters%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flol2.10026%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flol2.10026%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Copiotrophic%20marine%20bacteria%20are%20associated%20with%20strong%20iron-binding%20ligand%20production%20during%20phytoplankton%20blooms%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shane%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Randelle%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Although%20marine%20bacteria%20were%20identified%20nearly%20two%20decades%20ago%20as%20potential%20sources%20for%20strong%20iron-binding%20organic%20ligands%20detected%20in%20seawater%2C%20specific%20linkages%20between%20ligands%20detected%20in%20natural%20water%20and%20the%20microbial%20community%20remain%20unclear.%20We%20compared%20the%20production%20of%20different%20classes%20of%20iron-binding%20ligands%2C%20dissolved%20iron%20and%20macronutrient%20concentrations%2C%20and%20phytoplankton%20and%20bacterioplankton%20assemblages%20in%20a%20series%20of%20iron%20amended%206-d%20incubations.%20Incubations%20with%20high%20iron%20additions%20had%20near%20complete%20macronutrient%20consumption%20and%20higher%20phytoplankton%20biomass%20compared%20with%20incubations%20with%20low%20iron%20additions%2C%20but%20both%20iron%20treatments%20were%20dominated%20by%20diatoms.%20However%2C%20we%20only%20detected%20the%20strongest%20ligands%20in%20high-iron%20treatments%2C%20and%20strong%20iron-binding%20ligands%20were%20generally%20correlated%20with%20an%20increased%20abundance%20of%20copiotrophic%20bacteria%2C%20particularly%20Alteromonas%20strains.%20Ultimately%2C%20these%20robust%20correlations%20suggest%20a%20potential%20linkage%20between%20copiotrophic%20bacteria%20and%20strong%20iron-binding%20ligand%20production%20after%20iron%20fertilization%20events%20in%20the%20marine%20environment.%22%2C%22date%22%3A%222016%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Flol2.10026%22%2C%22ISSN%22%3A%222378-2242%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222023-05-03T20%3A39%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22CFQV9P6G%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Fuentes-Vald%5Cu00e9s%20et%20al.%22%2C%22parsedDate%22%3A%222016-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFuentes-Vald%26%23xE9%3Bs%2C%20J.%20J.%2C%20Plominsky%2C%20A.%20M.%2C%20%3Cstrong%3EAllen%2C%20E.%20E.%3C%5C%2Fstrong%3E%2C%20Tamames%2C%20J.%2C%20%26amp%3B%20V%26%23xE1%3Bsquez%2C%20M.%20%282016%29.%20Complete%20genome%20sequence%20of%20a%20cylindrospermopsin-producing%20cyanobacterium%2C%20Cylindrospermopsis%20raciborskii%20cs505%2C%20containing%20a%20circular%20chromosome%20and%20a%20single%20extrachromosomal%20element.%20%3Ci%3EGenome%20Announcements%3C%5C%2Fi%3E%2C%20%3Ci%3E4%3C%5C%2Fi%3E%284%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FgenomeA.00823-16%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FgenomeA.00823-16%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Complete%20genome%20sequence%20of%20a%20cylindrospermopsin-producing%20cyanobacterium%2C%20Cylindrospermopsis%20raciborskii%20cs505%2C%20containing%20a%20circular%20chromosome%20and%20a%20single%20extrachromosomal%20element%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juan%20J.%22%2C%22lastName%22%3A%22Fuentes-Vald%5Cu00e9s%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alvaro%20M.%22%2C%22lastName%22%3A%22Plominsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Javier%22%2C%22lastName%22%3A%22Tamames%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00f3nica%22%2C%22lastName%22%3A%22V%5Cu00e1squez%22%7D%5D%2C%22abstractNote%22%3A%22Cylindrospermopsis%20raciborskii%20is%20a%20freshwater%20cyanobacterium%20producing%20bloom%20events%20and%20toxicity%20in%20drinking%20water%20source%20reservoirs.%20We%20present%20the%20first%20genome%20sequence%20for%20C.%5Cu00a0raciborskii%20CS505%20%28Australia%29%2C%20containing%20one%204.1-Mbp%20chromosome%20and%20one%20110-Kbp%20plasmid%20having%20G%2BC%20contents%20of%2040.3%25%20%283933%20genes%29%20and%2039.3%25%20%28111%20genes%29%2C%20respectively.%22%2C%22date%22%3A%222016%5C%2F08%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FgenomeA.00823-16%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%5D%2C%22dateModified%22%3A%222022-05-24T22%3A37%3A17Z%22%7D%7D%5D%7D
Neu, A. T., Torchin, M. E., Allen, E. E., & Roy, K. (2024). Microbiome divergence of marine gastropod species separated by the Isthmus of Panama. Applied and Environmental Microbiology, 90(11), e01003-24. https://doi.org/10.1128/aem.01003-24
Suárez-Moo, P., García-Martínez, N. C., Márquez-Velázquez, N. A., Figueroa, M., Allen, E., & Prieto-Davó, A. (2024). Exploring the microbial community and biotechnological potential of the sponge Xestospongia sp. from an anchialine cave in the Yucatán Peninsula. Ciencias Marinas, 50. https://doi.org/10.7773/cm.y2024.3442
Oliver, A., Podell, S., Wegley Kelly, L., Sparagon, W. J., Plominsky, A. M., Nelson, R. S., Laurens, L. M. L., Augyte, S., Sims, N. A., Nelson, C. E., & Allen, E. E. (2024). Enrichable consortia of microbial symbionts degrade macroalgal polysaccharides in Kyphosus fish. MBio, e00496-24. https://doi.org/10.1128/mbio.00496-24
Podell, S., Oliver, A., Kelly, L. W., Sparagon, W. J., Plominsky, A. M., Nelson, R. S., Laurens, L. M. L., Augyte, S., Sims, N. A., Nelson, C. E., & Allen, E. E. (2023). Herbivorous Fish Microbiome Adaptations to Sulfated Dietary Polysaccharides. Applied and Environmental Microbiology, 89(5), e02154-22. https://doi.org/10.1128/aem.02154-22
Wilson, K., De Rond, T., Burkhardt, I., Steele, T. S., Schäfer, R. J. B., Podell, S., Allen, E. E., & Moore, B. S. (2023). Terpene biosynthesis in marine sponge animals. Proceedings of the National Academy of Sciences, 120(9), e2220934120. https://doi.org/10.1073/pnas.2220934120
Minich, J. J., Härer, A., Vechinski, J., Frable, B. W., Skelton, Z. R., Kunselman, E., Shane, M. A., Perry, D. S., Gonzalez, A., McDonald, D., Knight, R., Michael, T. P., & Allen, E. E. (2022). Host biology, ecology and the environment influence microbial biomass and diversity in 101 marine fish species. Nature Communications, 13(1), 6978. https://doi.org/10.1038/s41467-022-34557-2
Oliver, A., Cavalheri, H. B., Lima, T. G., Jones, N. T., Podell, S., Zarate, D., Allen, E., Burton, R. S., & Shurin, J. B. (2022). Phenotypic and transcriptional response of Daphnia pulicaria to the combined effects of temperature and predation. PLOS ONE, 17(7), e0265103. https://doi.org/10.1371/journal.pone.0265103
Kunselman, E., Minich, J. J., Horwith, M., Gilbert, J. A., & Allen, E. E. (2022). Variation in Survival and Gut Microbiome Composition of Hatchery-Grown Native Oysters at Various Locations within the Puget Sound. Microbiology Spectrum, e01982-21. https://doi.org/10.1128/spectrum.01982-21
Blanton, J. M., Peoples, L. M., Gerringer, M. E., Iacuaniello, C. M., Gallo, N. D., Linley, T. D., Jamieson, A. J., Drazen, J. C., Bartlett, D. H., & Allen, E. E. (2022). Microbiomes of Hadal Fishes across Trench Habitats Contain Similar Taxa and Known Piezophiles. MSphere, 7(2), e00032-22. https://doi.org/10.1128/msphere.00032-22
Sparagon, W. J., Gentry, E. C., Minich, J. J., Vollbrecht, L., Laurens, L. M. L., Allen, E. E., Sims, N. A., Dorrestein, P. C., Kelly, L. W., & Nelson, C. E. (2022). Fine scale transitions of the microbiota and metabolome along the gastrointestinal tract of herbivorous fishes. Animal Microbiome, 4(1), 33. https://doi.org/10.1186/s42523-022-00182-z
Klau, L. J., Podell, S., Creamer, K. E., Demko, A. M., Singh, H. W., Allen, E. E., Moore, B. S., Ziemert, N., Letzel, A. C., & Jensen, P. R. (2022). The Natural Product Domain Seeker version 2 (NaPDoS2) webtool relates ketosynthase phylogeny to biosynthetic function. Journal of Biological Chemistry, 298(10), 102480. https://doi.org/10.1016/j.jbc.2022.102480
Neu, A. T., Allen, E. E., & Roy, K. (2021). Defining and quantifying the core microbiome: Challenges and prospects. Proceedings of the National Academy of Sciences of the United States of America, 118(51), 10. https://doi.org/10.1073/pnas.2104429118
Neu, A. T., Allen, E. E., & Roy, K. (2021). Do host-associated microbes show a contrarian latitudinal diversity gradient? Insights from Mytilus californianus, an intertidal foundation host. Journal of Biogeography, 14. https://doi.org/10.1111/jbi.14243
Oliver, A., Podell, S., Pinowska, A., Traller, J. C., Smith, S. R., McClure, R., Beliaev, A., Bohutskyi, P., Hill, E. A., Rabines, A., Zheng, H., Allen, L. Z., Kuo, A., Grigoriev, I. V., Allen, A. E., Hazlebeck, D., & Allen, E. E. (2021). Diploid genomic architecture of Nitzschia inconspicua, an elite biomass production diatom. Scientific Reports, 11(1), 14. https://doi.org/10.1038/s41598-021-95106-3
Minich, J. J., Poore, G. D., Jantawongsri, K., Johnston, C., Bowie, K., Bowman, J., Knight, R., Nowak, B., Allen, E. E., & Liu, S.-J. (2021). Microbial ecology of Atlantic salmon (Salmo salar) hatcheries: Impacts of the built environment on fish mucosal microbiota. Applied and Environmental Microbiology, 86(12), e00411-20. https://doi.org/10.1128/AEM.00411-20
Allemann, M. N., Allen, E. E., & Atomi, H. (2021). Genetic suppression of lethal mutations in fatty acid biosynthesis mediated by a secondary lipid synthase. Applied and Environmental Microbiology, 87(12), e00035-21. https://doi.org/10.1128/AEM.00035-21
Petras, D., Minich, J. J., Cancelada, L. B., Torres, R. R., Kunselman, E., Wang, M. X., White, M. E., Allen, E. E., Prather, K. A., Aluwihare, L. I., & Dorrestein, P. C. (2021). Non-targeted tandem mass spectrometry enables the visualization of organic matter chemotype shifts in coastal seawater. Chemosphere, 271. https://doi.org/10.1016/j.chemosphere.2020.129450
Minich, J. J., Nowak, B., Elizur, A., Knight, R., Fielder, S., & Allen, E. E. (2021). Impacts of the marine hatchery built environment, water and feed on across ontogeny in yellowtail kingfish, Seriola lalandi. Frontiers in Marine Science, 8, 14. https://doi.org/10.3389/fmars.2021.676731
Minich, J., Nowak, B., Elizur, A., Knight, R., Fielder, S., & Allen, E. (2021). Impacts of the marine hatchery built environment on mucosal microbiome colonization across ontogeny in yellowtail kingfish, Seriola Lalandi. Research Square. https://doi.org/10.21203/rs.3.rs-75521/v1
Nayfach, S., Roux, S., Seshadri, R., Udwary, D., Varghese, N., Schulz, F., Wu, D., Paez-Espino, D., Chen, I. M., Huntemann, M., Palaniappan, K., Ladau, J., Mukherjee, S., Reddy, T. B. K., Nielsen, T., Kirton, E., Faria, J. P., Edirisinghe, J. N., Henry, C. S., … Img M. Data Consortium. (2021). A genomic catalog of Earth’s microbiomes. Nature Biotechnology, 39(4), 499–509. https://doi.org/10.1038/s41587-020-0718-6
Minich, J. J., Ali, F., Marotz, C., Belda-Ferre, P., Chiang, L., Shaffer, J. P., Carpenter, C. S., McDonald, D., Gilbert, J., Allard, S. M., Allen, E. E., Knight, R., Sweeney, D. A., & Swafford, A. D. (2021). Feasibility of using alternative swabs and storage solutions for paired SARS-CoV-2 detection and microbiome analysis in the hospital environment. Microbiome, 9(1). https://doi.org/10.1186/s40168-020-00960-4
Neu, A. T., Hughes, I. V., Allen, E. E., & Roy, K. (2021). Decade-scale stability and change in a marine bivalve microbiome. Molecular Ecology. https://doi.org/10.1111/mec.15796
Minich, J. J., Power, C., Melanson, M., Knight, R., Webber, C., Rough, K., Bott, N. J., Nowak, B., & Allen, E. E. (2020). The Southern Bluefin Tuna mucosal microbiome is influenced by husbandry method, net pen location, and anti-parasite treatment. Frontiers in Microbiology. https://doi.org/10.1101/2020.05.19.105270
Podell, S., Blanton, J. M., Oliver, A., Schorn, M. A., Agarwal, V., Biggs, J. S., Moore, B. S., & Allen, E. E. (2020). A genomic view of trophic and metabolic diversity in clade-specific Lamellodysidea sponge microbiomes. Microbiome, 8. https://doi.org/10.21203/rs.2.17204/v1
Allemann, M. N., & Allen, E. E. (2020). Genetic regulation of the bacterial omega-3 polyunsaturated fatty acid biosynthesis pathway. Journal of Bacteriology. https://doi.org/10.1128/JB.00050-20
Schulhof, M. A., Allen, A. E., Allen, E. E., Mladenov, N., McCrow, J. P., Jones, N. T., Blanton, J., Cavalheri, H. B., Kaul, D., Symons, C. C., & Shurin, J. B. (2020). Sierra Nevada mountain lake microbial communities are structured by temperature, resources and geographic location. Molecular Ecology. https://doi.org/10.1111/mec.15469
Fedarko, M. W., Martino, C., Morton, J. T., González, A., Rahman, G., Marotz, C. A., Minich, J. J., Allen, E. E., & Knight, R. (2020). Visualizing ’omic feature rankings and log-ratios using Qurro. NAR Genomics and Bioinformatics, 2(2). https://doi.org/10.1093/nargab/lqaa023
Minich, J., Petrus, S., Michael, J. D., Michael, T. P., Knight, R., & Allen, E. (2020). Temporal, environmental, and biological drivers of the mucosal microbiome in a wild marine fish, Scomber japonicus. MSphere, 5. https://doi.org/10.1128/mSphere.00401-20
Mohanty, I., Podell, S., Biggs, J. S., Garg, N., Allen, E. E., & Agarwal, V. (2020). Multi-omic profiling of melophlus sponges reveals diverse metabolomic and microbiome architectures that are non-overlapping with ecological neighbors. Marine Drugs, 18(2), 124. https://doi.org/10.3390/md18020124
Kolody, B. C., McCrow, J. P., Allen, L. Z., Aylward, F. O., Fontanez, K. M., Moustafa, A., Moniruzzaman, M., Chavez, F. P., Scholin, C. A., Allen, E. E., Worden, A. Z., Delong, E. F., & Allen, A. E. (2019). Diel transcriptional response of a California Current plankton microbiome to light, low iron, and enduring viral infection. The ISME Journal. https://doi.org/10.1038/s41396-019-0472-2
Schorn, M. A., Jordan, P. A., Podell, S., Blanton, J. M., Agarwal, V., Biggs, J. S., Allen, E. E., & Moore, B. S. (2019). Comparative genomics of cyanobacterial symbionts reveals distinct, specialized metabolism in tropical Dysideidae sponges. Mbio, 10. https://doi.org/10.1128/mBio.00821-19
Neu, A. T., Allen, E. E., & Roy, K. (2019). Diversity and composition of intertidal gastropod microbiomes across a major marine biogeographic boundary. Environmental Microbiology Reports, 11(3), 434–447. https://doi.org/10.1111/1758-2229.12743
Allemann, M. N., Shulse, C. N., & Allen, E. E. (2019). Linkage of marine bacteria polyunsaturated fatty acid and long-chain hydrocarbon biosynthesis. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00702
Peoples, L. M., Grammatopoulou, E., Pombrol, M., Xu, X. X., Osuntokun, O., Blanton, J., Allen, E. E., Nunnally, C. C., Drazen, J. C., Mayor, D. J., & Bartlett, D. H. (2019). Microbial community diversity within sediments from two geographically separated hadal trenches. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00347
Minich, J. J., Humphrey, G., Benitez, R. A. S., Sanders, J., Swofford, A., Allen, E. E., & Knight, R. (2018). High-throughput miniaturized 16S rRNA amplicon library preparation reduces costs while preserving microbiome integrity. MSystems, 3(6). https://doi.org/10.1128/mSystems.00166-18
Podell, S., Blanton, J. M., Neu, A., Agarwal, V., Biggs, J. S., Moore, B. S., & Allen, E. E. (2018). Pangenomic comparison of globally distributed Poribacteria associated with sponge hosts and marine particles. The ISME Journal. https://doi.org/10.1038/s41396-018-0292-9
Minich, J. J., Zhu, Q., Xu, Z. Z., Amir, A., Ngochera, M., Simwaka, M., Allen, E. E., Zidana, H., & Knight, R. (2018). Microbial effects of livestock manure fertilization on freshwater aquaculture ponds rearing tilapia (Oreochromis shiranus) and North African catfish (Clarias gariepinus). MicrobiologyOpen, 0(0), e00716. https://doi.org/10.1002/mbo3.716
Plominsky, A. M., Henriquez-Castillo, C., Delherbe, N., Podell, S., Ramirez-Flandes, S., Ugalde, J. A., Santibanez, J. F., van den Engh, G., Hanselmann, K., Ulloa, O., De la Iglesia, R., Allen, E. E., & Trefault, N. (2018). Distinctive archaeal composition of an artisanal crystallizer pond and functional insights into salt-saturated hypersaline environment adaptation. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.01800
Guillemette, R., Kaneko, R., Blanton, J., Tan, J., Witt, M., Hamilton, S., Allen, E. E., Medina, M., Hamasaki, K., Koch, B. P., & Azam, F. (2018). Bacterioplankton drawdown of coral mass-spawned organic matter. The ISME Journal. https://doi.org/10.1038/s41396-018-0197-7
Minich, J. J., Zhu, Q. Y., Janssen, S., Hendrickson, R., Amir, A., Vetter, R., Hyde, J., Doty, M. M., Stillwell, K., Benardini, J., Kim, J. H., Allen, E. E., Venkateswaran, K., & Knight, R. (2018). KatharoSeq enables high-throughput microbiome analysis from low-biomass samples. MSystems, 3(3). https://doi.org/10.1128/mSystems.00218-17
Peoples, L. M., Donaldson, S., Osuntokun, O., Xia, Q., Nelson, A., Blanton, J., Allen, E. E., Church, M. J., & Bartlett, D. H. (2018). Vertically distinct microbial communities in the Mariana and Kermadec trenches. PLOS ONE, 13(4). https://doi.org/10.1371/journal.pone.0195102
Plominsky, A. M., Trefault, N., Podell, S., Blanton, J. M., De la Iglesia, R., Allen, E. E., von Dassow, P., & Ulloa, O. (2018). Metabolic potential and in situ transcriptomic profiles of previously uncharacterized key microbial groups involved in coupled carbon, nitrogen and sulfur cycling in anoxic marine zones. Environmental Microbiology. https://doi.org/10.1111/1462-2920.14109
Allemann, M. N., & Allen, E. E. (2018). Characterization and Application of Marine Microbial Omega-3 Polyunsaturated Fatty Acid Synthesis. In B. S. Moore (Ed.), Methods in Enzymology (Vol. 605, pp. 3–32). Academic Press.
Leao, T., Castelao, G., Korobeynikov, A., Monroe, E. A., Podell, S., Glukhov, E., Allen, E. E., Gerwick, W. H., & Gerwick, L. (2017). Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genus Moorea. Proceedings of the National Academy of Sciences of the United States of America, 114(12), 3198–3203. https://doi.org/10.1073/pnas.1618556114
Agarwal, V., Blanton, J. M., Podell, S., Taton, A., Schorn, M. A., Busch, J., Lin, Z., Schmidt, E. W., Jensen, P. R., Paul, V. J., Biggs, J. S., Golden, J. W., Allen, E. E., & Moore, B. S. (2017). Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges. Nat Chem Biol, advance online publication. https://doi.org/10.1038/nchembio.2330
Demidenko, A., Akberdinl, I. R., Allemann, M., Allen, E. E., & Kalyuzhnaya, M. G. (2017). Fatty acid biosynthesis pathways in Methylomicrobium buryatense 5G(B1). Frontiers in Microbiology, 7. https://doi.org/10.3389/fmicb.2016.02167
Paerl, R. W., Bouget, F.-Y., Lozano, J.-C., Verge, V., Schatt, P., Allen, E. E., Palenik, B., & Azam, F. (2016). Use of plankton-derived vitamin B1 precursors, especially thiazole-related precursor, by key marine picoeukaryotic phytoplankton. Isme Journal. https://doi.org/10.1038/ismej.2016.145
Traller, J. C., Cokus, S. J., Lopez, D. A., Gaidarenko, O., Smith, S. R., McCrow, J. P., Gallaher, S. D., Podell, S., Thompson, M., Cook, O., Morselli, M., Jaroszewicz, A., Allen, E. E., Allen, A. E., Merchant, S. S., Pellegrini, M., & Hildebrand, M. (2016). Genome and methylome of the oleaginous diatom Cyclotella cryptica reveal genetic flexibility toward a high lipid phenotype. Biotechnology for Biofuels, 9(1), 258. https://doi.org/10.1186/s13068-016-0670-3
Hogle, S. L., Bundy, R. M., Blanton, J. M., Allen, E. E., & Barbeau, K. A. (2016). Copiotrophic marine bacteria are associated with strong iron-binding ligand production during phytoplankton blooms. Limnology and Oceanography Letters. https://doi.org/10.1002/lol2.10026
Fuentes-Valdés, J. J., Plominsky, A. M., Allen, E. E., Tamames, J., & Vásquez, M. (2016). Complete genome sequence of a cylindrospermopsin-producing cyanobacterium, Cylindrospermopsis raciborskii cs505, containing a circular chromosome and a single extrachromosomal element. Genome Announcements, 4(4). https://doi.org/10.1128/genomeA.00823-16